KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1418 | - |
N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide | pH 7.0, 37°C | Homo sapiens | |
0.1818 | - |
N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide | pH 4.5, 37°C | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate | ? | - |
? | |
additional information | Mus musculus | the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NR71 | - |
- |
Mus musculus | Q9WV54 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
3T3 cell | - |
Mus musculus | - |
commercial preparation | recombinant enzyme | Homo sapiens | - |
additional information | the nCDase subtype is a ubiquitously expressed enzyme | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-N-[(2S,3R,4E)-1,3-dihydroxy-7-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hept-4-en-2-yl]undecanamide + H2O | - |
Homo sapiens | ? | - |
ir | |
11-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl]oxy]-N-[(2S,3R,4E)-1,3-dihydroxy-7-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hept-4-en-2-yl]undecanamide + H2O | - |
Mus musculus | ? | - |
ir | |
additional information | the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate | Homo sapiens | ? | - |
? | |
additional information | the N-acyl moiety of ceramide is cleaved by ceramidases to form the lysolipid sphingosine that undergoes further phosphorylation to sphingosine-1-phosphate | Mus musculus | ? | - |
? | |
N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide + H2O | fluorescently labelled ceramide analogue, 4.0% hydrolýsis | Homo sapiens | ? | - |
ir | |
N-[(2S,3R,4E)-7-[[9-(diethylamino)-5-oxo-5H-benzo[a]phenoxazin-3-yl]oxy]-1,3-dihydroxyhept-4-en-2-yl]-11-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]undecanamide + H2O | fluorescently labelled ceramide analogue, 5.8% hydrolýsis | Mus musculus | ? | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
aCDase | - |
Mus musculus |
acid ceramidase | - |
Mus musculus |
nCDase | - |
Homo sapiens |
neutral ceramidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Mus musculus |
7 | - |
assay at | Homo sapiens |