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Literature summary for 3.5.1.23 extracted from
- Molecular cloning and characterization of neutral ceramidase homologue from the red flour beetle, Tribolium castaneum (2011), Biochimie, 93, 1124-1131.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Tn5B-1-4 cells (High Five cells) | Tribolium castaneum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 70% residual activity at 5 mM | Tribolium castaneum |  |
| Fe2+ | about 50% residual activity at 5 mM | Tribolium castaneum | |
| Mg2+ | about 72% residual activity at 5 mM | Tribolium castaneum | |
| Mn2+ | about 90% residual activity at 5 mM | Tribolium castaneum | |
| Zn2+ | about 87% residual activity at 5 mM | Tribolium castaneum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Tribolium castaneum | 5886 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tribolium castaneum | - |
red flour beetle | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| head | - |
Tribolium castaneum | - |
| midgut | - |
Tribolium castaneum | - |
| ovary | highest expression in females | Tribolium castaneum | - |
| spermary | highest expression in males | Tribolium castaneum | - |
| thorax | - |
Tribolium castaneum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme prefers medium-chain or long chain ceramides over short-chain ceramides as substrates | Tribolium castaneum | ? | - |
? | |
| N-caproylsphingosine + H2O | about 25% activity compared to N-oleoylsphingosine | Tribolium castaneum | caproate + sphingosine | - |
? | |
| N-lauroylsphingosine + H2O | about 95% activity compared to N-oleoylsphingosine | Tribolium castaneum | laurate + sphingosine | - |
? | |
| N-nervonoylsphingosine + H2O | about 80% activity compared to N-oleoylsphingosine | Tribolium castaneum | nervonate + sphingosine | - |
? | |
| N-oleoylsphingosine + H2O | 100% activity | Tribolium castaneum | oleate + sphingosine | - |
? | |
| N-palmitoylsphingosine + H2O | about 65% activity compared to N-oleoylsphingosine | Tribolium castaneum | palmitate + sphingosine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| neutral ceramidase | - |
Tribolium castaneum |
| Tncer | - |
Tribolium castaneum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
around 37°C | Tribolium castaneum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 12 | - |
Tribolium castaneum |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 5 | - |
pH and temperature not specified in the publication | Tribolium castaneum | Fe2+ | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Tribolium castaneum | mRNA levels are higher in the adult stage than in pre-adult stages | up |
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