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Literature summary for 3.5.1.23 extracted from

  • Lucki, N.C.; Bandyopadhyay, S.; Wang, E.; Merrill, A.H.; Sewer, M.B.
    Acid ceramidase (ASAH1) is a global regulator of steroidogenic capacity and adrenocortical gene expression (2012), Mol. Endocrinol., 26, 228-243.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.23

Activating Compound

Activating Compound Comment Organism Structure
additional information ACTH/cAMP signaling stimulates the enzyme transcription and activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene ASAH1, expression analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of a tetracycline-inducible ASAH1 short hairpin RNA H295R human adrenocortical stable cell line Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ceramide + H2O Homo sapiens
-
 sphingosine + fatty acid
-
 ?
ceramide + H2O Homo sapiens
-
 carboxylate + sphingosine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q13510 gene ASAH1
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal cortex
-
 Homo sapiens
-
adrenocortical carcinoma cell line
-
 Homo sapiens
-
NCI-H295R cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ceramide + H2O
-
 Homo sapiens sphingosine + fatty acid
-
 ?
ceramide + H2O
-
 Homo sapiens carboxylate + sphingosine
-
 ?

Synonyms

Synonyms Comment Organism
acid ceramidase
-
 Homo sapiens
ASAH1
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens ACTH/cAMP signaling stimulates acid ceramidase ASAH1 transcription and activity up
Homo sapiens ACTH/cAMP signaling stimulates the enzyme transcription and activity up

General Information

General Information Comment Organism
malfunction acid ceramidase ASAH1 knockdown leads to a decrease in cell proliferation with a concomitant reduction in the protein levels of BETA-catenin, proliferating cell nuclear antigen, and cyclin B2. ASAH1 silencing increases basal and cAMP-dependent cortisol and dehydroepiandrosterone secretion Homo sapiens
malfunction a tetracycline-inducible ASAH1 short hairpin RNA H295R human adrenocortical stable cell line shows increased transcription of multiple steroidogenic genes, including Cytochrome P450 monooxygenase (CYP)17A1, CYP11B1/2, CYP21A2, steroidogenic acute regulatory protein, hormone-sensitive lipase, 18-kDa translocator protein, and the melanocortin-2 receptor. Induced gene expression positively correlates with enhanced histone H3 acetylation at target promoters. Repression of ASAH1 expression also induces the expression of members of the nuclear receptor nuclear receptor subfamily 4 family while concomitantly suppressing the expression of dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1. ASAH1 knockdown alters the expression of genes involved in sphingolipid metabolism and changes the cellular amounts of distinct sphingolipid species. Enzyme silencing increases basal and cAMP-dependent cortisol and dehydroepiandrosterone secretion, establishing ASAH1 as a pivotal regulator of steroidogenic capacity in the human adrenal cortex Homo sapiens
physiological function acid ceramidase ASAH1 is a global regulator of steroidogenic capacity and adrenocortical gene expression Homo sapiens
physiological function acid ceramidase directly regulates the intracellular balance of ceramide, sphingosine, and sphingosine-1-phosphate by catalyzing the hydrolysis of ceramide into sphingosine. The enzyme plays a role in glucocorticoid production and regulating steroidogenic capacity Homo sapiens