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Literature summary for 3.5.1.23 extracted from
- Reverse hydrolysis reaction of a recombinant alkaline ceramidase of Pseudomonas aeruginosa (2000), Biochim. Biophys. Acta, 1485, 111-120.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulates ceramide synthesis | Pseudomonas aeruginosa |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli JM109 | Pseudomonas aeruginosa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide | modification of carboxyl groups completely inhibits forward and reverse reaction | Pseudomonas aeruginosa | |
| 2,3-Butanedione | modification of Arg causes about 50% inhibition of forward and reverse reaction | Pseudomonas aeruginosa | |
| N-bromosuccinimide | modification of Trp completely inhibits forward and reverse reaction | Pseudomonas aeruginosa | |
| sodium cholate | ceramide synthesis | Pseudomonas aeruginosa | |
| taurodeoxycholate | ceramide synthesis | Pseudomonas aeruginosa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Pseudomonas aeruginosa | |
| Mg2+ | can partially replace Ca2+ | Pseudomonas aeruginosa | |
| Mn2+ | can partially replace Ca2+ | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acylsphingosine + H2O | Pseudomonas aeruginosa | involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate | a carboxylate + sphingosine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-erythro-sphingosine + (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexa-4,7,10,13,16,19-enoic acid | 50% of activity | Pseudomonas aeruginosa | N-((4Z,7Z,10Z,13Z,16Z,19Z)-docosahexa-4,7,10,13,16,19-enoyl)-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + arachidonic acid | 61% of activity | Pseudomonas aeruginosa | N-arachidonyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + behenic acid | 24% of activity | Pseudomonas aeruginosa | N-behenoyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + cerotic acid | 4% of activity | Pseudomonas aeruginosa | N-cerotoyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + eicosapentaenoic acid | 52% of activity | Pseudomonas aeruginosa | N-eicosapentaenyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + fatty acid | - |
Pseudomonas aeruginosa | N-acyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + lauric acid | 72% of activity | Pseudomonas aeruginosa | N-lauroyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + lignoceric acid | 6% of activity | Pseudomonas aeruginosa | N-lignoceroyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + linoleic acid | 66% of activity | Pseudomonas aeruginosa | N-linoleoyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + myristic acid | best substrate | Pseudomonas aeruginosa | N-myristoyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + oleic acid | 39% of activity | Pseudomonas aeruginosa | N-oleoyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + palmitic acid | 70% of activity, palmitic acid can not be replaced with palmityl-CoA | Pseudomonas aeruginosa | N-palmitoyl-D-erythro-sphingosine + H2O | - |
r | |
| D-erythro-sphingosine + stearic acid | 49% of activity | Pseudomonas aeruginosa | N-stearoyl-D-erythro-sphingosine + H2O | - |
r | |
| N-acylsphingosine + H2O | - |
Pseudomonas aeruginosa | a carboxylate + sphingosine | - |
r | |
| N-acylsphingosine + H2O | involved in the biosynthesis of ceramide, crucial enzyme for the regulation of the intracellular balance of the contents of ceramide, sphingosine and possibly sphingosine 1-phosphate | Pseudomonas aeruginosa | a carboxylate + sphingosine | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ceramide synthesis | Pseudomonas aeruginosa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | ceramide synthesis | Pseudomonas aeruginosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor required | Pseudomonas aeruginosa |
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