Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pseudomonas aeruginosa | |
Triton X-100 | - |
Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Pseudomonas aeruginosa | |
EDTA | - |
Pseudomonas aeruginosa | |
EGTA | - |
Pseudomonas aeruginosa | |
Hg2+ | - |
Pseudomonas aeruginosa | |
N-oleoylethanolamine | - |
Pseudomonas aeruginosa | |
phytosphingosine | - |
Pseudomonas aeruginosa | |
sphinganine | - |
Pseudomonas aeruginosa | |
sphingosine | - |
Pseudomonas aeruginosa | |
Zn2+ | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.139 | - |
N-palmitoylsphingosine | - |
Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pseudomonas aeruginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | after EDTA treatment, Ca2+ reestablishes activity completely | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
83400fold | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture filtrate | - |
Pseudomonas aeruginosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
186 | - |
- |
Pseudomonas aeruginosa |
Storage Stability | Organism |
---|---|
-85°C, 2 months, with 1 mg/ml bovine serum albumin, no loss of activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-nitrobenz-2-oxa-1,3-diazole-N-lauroylsphingosine + H2O | - |
Pseudomonas aeruginosa | ? | - |
r | |
N-palmitoylphytosphingosine + H2O | - |
Pseudomonas aeruginosa | ? | - |
r | |
N-palmitoylsphinganine + H2O | - |
Pseudomonas aeruginosa | palmitic acid + sphinganine | - |
r | |
N-palmitoylsphingosine + H2O | - |
Pseudomonas aeruginosa | palmitic acid + sphingosine | - |
r | |
N-stearoylphytosphingosine + H2O | - |
Pseudomonas aeruginosa | ? | - |
r | |
N-stearoylsphinganine + H2O | - |
Pseudomonas aeruginosa | stearic acid + sphinganine | - |
r | |
N-stearoylsphingosine + H2O | - |
Pseudomonas aeruginosa | stearic acid + sphingosine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
24 | - |
stable at 24°C for 12 h | Pseudomonas aeruginosa |
37 | - |
total loss of activity after 12 h at 37°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas aeruginosa |