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3.5.1.23: ceramidase

This is an abbreviated version!
For detailed information about ceramidase, go to the full flat file.

Word Map on EC 3.5.1.23

Reaction

a ceramide
+
H2O
=
a carboxylate
+
sphingosine

Synonyms

(dihydro)ceramidase, AC, aCDase, ACER, ACER type 3, ACER-2, ACER1, ACER2, ACER3, acid ceramidase, acylsphingosine deacylase, adiponectin receptor, ADIPOR2, alkaline ceramidase, alkaline ceramidase 1, alkaline ceramidase 2, alkaline ceramidase 3, alkCDase, alkCDase-1, alkCDase-2, ASAH-1, ASAH1, asah1b, ASAH2, ASAH2B, ASAH3, AsahA, AtACER, AtNCER1, bwa, CBCDase, CDase, CDase II, CER2, ceramidase, Dacer, Drosophila alkaline ceramidase, glycosphingolipid ceramide deacylase, Golgi alkaline ceramidase, haCER1, intramembrane ceramidase, LsnCer, maCER1, mt-CDase, N-acylsphingosine amidohydrolase, N-acylsphingosine amidohydrolase 2, N-acylsphingosine amidohydrolase-1, N-acylsphingosine aminohydrolase, N-acylsphingosine deacylase, N-CDase, nCDase, ncer1, neutral CDase, neutral ceramidase, neutral ceramidase 2, NlnCDase, OsCDase, PaCD, PaCDase, pan-ceramidase, PHP32, phytoalkaline ceramidase, phytoCDase, Putative 32 KDA heart protein, SCDase, sphingolipid ceramide N-deacylase, Tncer, TOD1, turgor regulation defect 1, Ydc1p, yeast dihydroceramidase 1, yeast phyto-ceramidase 1, Ypc1p, znCD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.23 ceramidase

Purification

Purification on EC 3.5.1.23 - ceramidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
112 kDa-form from kidney: 3604fold, completely insoluble by freeze-thawing in contrast to liver CDase
1133.54fold, isoenzyme I, 404.32fold, isoenzyme II
-
151182fold
-
4454fold
-
501fold
-
concanavalin A column chromatography, Blue Sepharose column chromatography, and Superose 12 gel filtration
-
native enzyme 20000fold from brain by anion exchange chromatography, affinity and hydrophobic interaction chromatography, and cation exchange chromatography, to homogeneity
native enzyme 2147.1fold from brain to homogeneity by anion exchange and hydrophobic interaction chromtography, gel filtration, and another step of anion exchange chromatography
-
native enzyme from brain
native intestinal enzyme over 307fold by acetone precipitation and ion-exchange, hydrophobic-interaction chromatography, and gel filtration, to homogeneity
-
Q-Sepharose column chromatography, Superdex 200 gel filtration, heparin-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Mono Q column chromatography
-
recombinant AC expressed in CHO cells from the culture media, 58fold
-
recombinant BRIL-fusion FLAG-tagged wild-type and mutant enzymes from Spodoptera frugiperda Sf9 cells by immunoaffinity and FLAG-tag affinity chromatography, the FLAG-tag is cleaved off by TEV protease, followed by gel filtration. The purified E33G ACER3 mutant is highly unstable during purification
recombinant C-terminally His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography, ultrafiltration, and gel filtration
recombinant enzyme from High Five insect cells
recombinant enzyme from insect cells
recombinant enzyme from Trichopulsia ni High Five cell membranes
recombinant enzyme partially from SF9 cells
recombinant enzyme, large-scale purification
-
recombinant HA- and His6-tagged AsahA 90fold from Aspergillus oryzae
recombinant His-tagged CDase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged wild-type and mutant enzymes by nickel affinity chromatography and gel filtration
recombinant protein
-
recombinant secreted His6-tagged nCDase (residues 99-780) from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography and gel filtration
using a Ni-Sepharose 6 Fast Flow and a Superdex 200 HR column
wild-type CDase from 3-L culture: 217fold, recombinant CDase expressed in Escherichia coli