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3.5.1.23: ceramidase

This is an abbreviated version!
For detailed information about ceramidase, go to the full flat file.

Word Map on EC 3.5.1.23

Reaction

a ceramide
+
H2O
=
a carboxylate
 +
sphingosine

Synonyms

(dihydro)ceramidase, AC, aCDase, ACER, ACER type 3, ACER-2, ACER1, ACER2, ACER3, acid ceramidase, acylsphingosine deacylase, adiponectin receptor, ADIPOR2, alkaline ceramidase, alkaline ceramidase 1, alkaline ceramidase 2, alkaline ceramidase 3, alkCDase, alkCDase-1, alkCDase-2, ASAH-1, ASAH1, asah1b, ASAH2, ASAH2B, ASAH3, AsahA, AtACER, AtNCER1, bwa, CBCDase, CDase, CDase II, CER2, ceramidase, Dacer, Drosophila alkaline ceramidase, glycosphingolipid ceramide deacylase, Golgi alkaline ceramidase, haCER1, intramembrane ceramidase, LsnCer, maCER1, mt-CDase, N-acylsphingosine amidohydrolase, N-acylsphingosine amidohydrolase 2, N-acylsphingosine amidohydrolase-1, N-acylsphingosine aminohydrolase, N-acylsphingosine deacylase, N-CDase, nCDase, ncer1, neutral CDase, neutral ceramidase, neutral ceramidase 2, NlnCDase, OsCDase, PaCD, PaCDase, pan-ceramidase, PHP32, phytoalkaline ceramidase, phytoCDase, Putative 32 KDA heart protein, SCDase, sphingolipid ceramide N-deacylase, Tncer, TOD1, turgor regulation defect 1, Ydc1p, yeast dihydroceramidase 1, yeast phyto-ceramidase 1, Ypc1p, znCD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.23 ceramidase

Molecular Weight

Molecular Weight on EC 3.5.1.23 - ceramidase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
110000
112000
x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE
113000
x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE
116000
13000
133000
x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE
135000
-
determined by SDS-PAGE and Western blot analysis, the multiple band pattern between about 95 and 135 kDa may be due to the complexity of modifications, such as O-glycosylation and N-glycosylation
14000
1 * 14000 + 1 * 40000, SDS-PAGE
150000
28000
apparent molecular weight, deglycosylated beta subunit
31000
31300
theoretical value, Western blot analysis shows a molecular mass close to the predicted one
31600
theoretical value, Western blot analysis shows a molecular mass close to the predicted one
34000
x * 34000, recombinant enzyme, SDS-PAGE
40000
50000
53000
54000
synthesized as a polypeptide of 395 amino acids, 53 - 55 kDa
62000
-
isoenzyme II, gel filtration
70000
71000
-
x * 71000, recombinant enzyme, SDS-PAGE
79300
x * 79300, about, sequence calculation
80000
-
x * 100000, about, glycosylated enzyme, SDS-PAGE, x * 80000, about, deglycosylated enzyme, SDS-PAGE
82500
-
x * 110000, glycosylated recombinant enzyme, SDS-PAGE, x * 82500, deglycosylated recombinant enzyme, SDS-PAGE
83483
x * 112000, membrane-bound CDase from kidney, SDS-PAGE, x * 83483, CDase from kidney, sequence calculation, x * 113000, endoplasmic reticulum-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE, x * 133000, Golgi-form of Myc-tagged CDase expressed in HEK-239, SDS-PAGE
87000
-
determined by SDS-PAGE and Western blot analysis, signal between 75 and 100 kDa
90000
-
SDS-PAGE
94000
-
x * 94000, SDS-PAGE
95000
97000