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3.5.1.23: ceramidase

This is an abbreviated version!
For detailed information about ceramidase, go to the full flat file.

Word Map on EC 3.5.1.23

Reaction

a ceramide
+
H2O
=
a carboxylate
 +
sphingosine

Synonyms

(dihydro)ceramidase, AC, aCDase, ACER, ACER type 3, ACER-2, ACER1, ACER2, ACER3, acid ceramidase, acylsphingosine deacylase, adiponectin receptor, ADIPOR2, alkaline ceramidase, alkaline ceramidase 1, alkaline ceramidase 2, alkaline ceramidase 3, alkCDase, alkCDase-1, alkCDase-2, ASAH-1, ASAH1, asah1b, ASAH2, ASAH2B, ASAH3, AsahA, AtACER, AtNCER1, bwa, CBCDase, CDase, CDase II, CER2, ceramidase, Dacer, Drosophila alkaline ceramidase, glycosphingolipid ceramide deacylase, Golgi alkaline ceramidase, haCER1, intramembrane ceramidase, LsnCer, maCER1, mt-CDase, N-acylsphingosine amidohydrolase, N-acylsphingosine amidohydrolase 2, N-acylsphingosine amidohydrolase-1, N-acylsphingosine aminohydrolase, N-acylsphingosine deacylase, N-CDase, nCDase, ncer1, neutral CDase, neutral ceramidase, neutral ceramidase 2, NlnCDase, OsCDase, PaCD, PaCDase, pan-ceramidase, PHP32, phytoalkaline ceramidase, phytoCDase, Putative 32 KDA heart protein, SCDase, sphingolipid ceramide N-deacylase, Tncer, TOD1, turgor regulation defect 1, Ydc1p, yeast dihydroceramidase 1, yeast phyto-ceramidase 1, Ypc1p, znCD

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.23 ceramidase

Cloned

Cloned on EC 3.5.1.23 - ceramidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
112 kDa-form from kidney, expression in Escherichia coli BL21(DE3), in CHOP cells and overexpression in HEK-239 cells
acid ceramidase is expressed in Sf21 insect cells
acid ceramidase maps to 8p22, the region of chromosome 8 most frequently deleted in prostate cancer. Regulation of gene expression by acid ceramidase in prostate cancer cells, overview
-
cloned from a brain cDNA library, stable overexpression of V5/His-tagged enzyme in HEK-293 cells
cloning of human nCDase gene in pLenti6.3/TO/V5 and recombinant expression in HCT-116 cells and in cells transfected with the Golgi-targeted bacterial ceramidase and sphingomyelinase
DNA and amino acid determination, phylogenetic tree
DNA and amino acid determination, phylogenetic tree, genetic structure
DNA and amino acid determination, phylogenetic tree, genetic structure, the promoter region of mouse brain neutral CDase contains transcriptional response elements for GATA-2, C/EBP, and HNF3beta
DNA and amino acid determination, phylogenetic tree, recombinant expression of myc-tagged or GFP-tagged neutral CDase in HEK293 cells. When expressed in HEK293 or CHOP cells, both wild-type and mutant rat neutral CDase with a deleted mucin box are released into the medium
DNA and amino acid sequence determination and analysis, overexpression of C-terminally myc-tagged enzyme in CHOP cells and zebrafish BRF41 cells in endoplasmic reticulum and Golgi apparatus, as well as in plasma membranes
-
DNA and amino acid sequence determination and analysis, recombinant overexpression in Trichopulsia ni High Five cell membranes via baculoviral transfection, expression of GFP-tagged enzyme
expressed in CHO cells
-
expressed in Escherichia coli JM109
-
expressed in Tn5B-1-4 cells (High Five cells)
-
expression analysis of isozymes during keratinocyte differentiation, expression levels in undifferentiated, early-stage and advanced-stage cells, and in whole epidermis by RT-PCR
expression in Escherichia coli
-
expression in Escherichia coli BL21(DE3)
-
expression in Escherichia coli DH5alpha and BL21, expression at low levels in Pseudomonas putida KT2440 and Pichia pastoris GS115, sequencing
expression in Saccharomyces cerevisiae enzyme-deficient doubleknockout strain.ypc1.ydc1 microsomes under control of the Gal1 promoter
expression of C-terminally myc-tagged enzyme in CHOP cells in endoplasmic reticulum and Golgi apparatus, as well as in plasma membranes
-
expression of FLAG-tagged wild-type ACER2 and FLAG-tagged ACER2 mutants in Saccharomyces cerevisiae mutant cell microsomes, DELTAypc1 and DELTAydc1, that lack endogenous ceramidase activity
-
expression of His6-tagged CDase in Escherichia coli strain BL21(DE3)
-
expression of the N-terminally GFP-tagged enzyme lacking the 19 amino acid N-terminal extension in HEK-293 and MCF7 cells exclusively in mitohcondria, expression of the longer enzyme version cloned from kidney resulted in localization of the recombinant enzyme in the plasma membrane of HEK-293 cells
for expression in human HEK-293 cells
functional overexpression of N-terminally C-myc-tagged enzyme, residues 1-1218, in 293T cells using a retroviral vector, in cell culture supernatant, recombinant retrovirus from 293T cell supernatant is used to infect C2C12 myoblasts
-
gene ASAH1, DNA and amino acid sequence determination and analysis, genotyping, semi-quantitative enzyme expression analysis
gene ASAH1, expression analysis
gene ASAH1, in vitro translation of FLAG-tagged enzyme from the pCMV6-XL5-ASAH1 plasmid and transient expression in H295R cells
gene ASAH1, quantitative RT-PCR enzyme expression analysis
gene ASAH1, recombinant overexpression in HEK-293 cells from pLenti6.3/TO/V5-DEST vector
gene ASAH1, recombinant overexpression in HEK-293T/17 cells, quantitative real-time RT-PCR enzyme expression analysis
gene asah1b, DNA and amino acid sequence determination and analysis, recombinant overexpression of C-terminally His-tagged enzyme in Pichia pastoris strain GS115, method optimization, overview
gene Asah2, DNA and amino acid sequence determination and analysis, expression in ES cells
-
gene asahA, DNA and amino acid sequence determination and analysis, overexpression of HA- and His6-tagged neutral CDase in Aspergillus oryzae
gene At4G22330 or acer-1, phylogenetic analysis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
gene bwa, recombinant expression of His-tagged wild-type and mutant enzymes, the endogenous signal peptide comprising the first 21 residues is replaced by the melittin signal peptide (MKFLVNVALVFMVVYISYIYA) followed by a His6-tag. Constructs encompassed residues 22 to 395 of homologues from human (UniProt Q13510 variant Ile93Val), nmr (Heterocephalus glaber, UniProt A0A0P6JG37 variant Asn348Ser), or cmw (Balaenoptera acutorostrata scammoni, RefSeq XP_007174053). The latter two homologues are codon-optimized
gene bwa, recombinant expression of wild-type and mutant enzymes in Spodoptera frugiperda Sf9 cells via baculovirus transfection system, the enzyme is cloned with C-terminal BRIL soluble module (thermostabilized apocytochrome b562RIL) and an N-terminal Flag epitope followed by a tobacco etch virus protease site
gene cdase, the gene encoding the enzyme is adjacent to that encoding hemolytic phospholipase C, plcH, in the genome of Pseudomonas aeruginosa
-
gene ncer1, transient expression of GFP-tagged enzyme in Arabidosis thaliana protoplasts in the endoplasmic reticulum, quantitative real-time PCR enzyme expression analysis
gene NlnCDase, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the enzyme in insect High Five cells in microsomes, quantitative enzyme expression analysis
-
gene tod1, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic tree
gene YPC, recombinant expression of HA7-tagged enzyme
into the vector pET23a for expression in Escherichia coli BL21DE3 pLysS cells, in B834DE3 pLysS cells for preparation of selenomethionine-substituted enzyme
into the yeast expression vector pYES2/CT
-
maCER1 gene, overexpression in HeLa cells, expression in yeast cells and in COS-1 cells, sequencing
overexpression in CHO cells
-
overexpression in CHO cells and in normal human skin fibroblasts
-
radiation-induced acid ceramidase gene transactivation by activator protein 1 binding on the proximal promoter is sensitive to inhibition of de novo ceramide biosynthesis, as demonstrated by promoter reporter and ChIP-qPCR analyses
recombinant expression in insect cells
recombinant expression in insect cells of active truncated enzyme, consisting of the extracellular region (residues 99-780) and lacking the short intracellular region (residues 1-12), the transmembrane domain (residues 12-34), and the flexible O-glycosylated mucin box (residues 34-99)
recombinant expression in Spodoptera frugiperda Sf9 cells
recombinant expression of adenoviral vector expressing the human acid ceramidase (Ad-ASAH1) gene in human OBA-9 cells. ASAH1-over-expressing OBA-9 cells show significantly lower mRNA expressions of caspase-3 as well as the percentage of Annexin V-positive cells, when compared to controls. In response to stimulation with Porphyromonas gingivalis, ASAH1-overexpressing OBA-9 cells produce less TNF-alpha, IL-6, and IL1beta pro-inflammatory cytokines than observed in OBA-9 cells transduced with Ad-control vector, quantitative real-time PCR expression analysis
recombinant expression of V5/His-tagged enzyme using vector pEF6-V5/His in HEK-293 cells
recombinant overexpression of C-terminally His6-tagged extracellular region of human nCDase (residues 99-780) lacking the flexible O-glycosylated mucin box in Spodoptera frugiperda Sf9 cells, the enzyme is secreted
the Dacer/brainwashing or BWA gene CG13969 localizes to the 38B2-3 region of the left arm of the second chromosome, DNA and amino acid sequence determination and analysis, functional overexpression in High Five insect cells
the gene encoding CER2 is located on chromosome 9, cloning from a liver cDNA library, functional expression of CER2 in HeLa cells showing 20fold increased enzyme activity in microsomes
the human ASAH1 promoter is cloned
-
transient expression of FLAG-tagged ACER3 in HSC-1 cells. ACER2 overexpression in HeLa cells, knockdown by RNA interference with ACER2-specific siRNA
-
transient overexpression of FLAG-tagged ACER3 in HSC-1 cells
-
wild-type and C-terminal truncated CDase, expression in HEK-293 cells
-