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Information on EC 3.4.11.1 - leucyl aminopeptidase
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3.4.11.1 leucyl aminopeptidaseSpecify your search results
Word Map
- 3.4.11.1
- aminopeptidases
- border
-
brush
- bestatin
- transpeptidase
-
gamma-glutamyl
- maltase
- proteases
- sucrase
- carboxypeptidase
- urinary
- pregnancy
- urine
- esterase
- bile
- transaminase
-
trim
- chymotrypsin
- mucosa
- cathepsins
-
dipeptidyl
- beta-glucuronidase
-
exopeptidase
- 5'-nucleotidase
-
brush-border
-
histocompatibility
- bilirubin
-
nephrotoxicity
- gamma-glutamyltransferase
-
ankylosing
- trehalase
- n-acetyl-beta-glucosaminidase
- amastatin
-
canalicular
- gamma-gtp
-
3.4.11.2
- disaccharidase
- gamma-glutamyltranspeptidase
- oxytocinase
-
jaundice
- dipeptidase
- p-nitroanilide
-
alanyl
- cholinesterase
- spondylitis
- hepatobiliary
- arylamidase
- fasciola
-
enzymuria
- hepatica
- drug development
- pharmacology
- medicine
- food industry
- diagnostics
- synthesis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
Synonyms
A-LAP, acidic M17 leucine aminopeptidase, AcLAP, adipocyte-derived leucine aminopeptidase, Aminopeptidase, aminopeptidase A, Aminopeptidase A/I, aminopeptidase I, aminopeptidase II, Aminopeptidase III, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
A-LAP
acidic M17 leucine aminopeptidase
adipocyte-derived leucine aminopeptidase
aminopeptidase A
aminopeptidase I
aminopeptidase II
Aminopeptidase III
APDkam589
cathepsin III
cytosol aminopeptidase
FrvX
L-leucine aminopeptidase
LAP
LAP-A
LAP1
lap3
leucinamide aminopeptidase
leucinaminopeptidase
leucine aminopeptidase
leucine aminopeptidase 1
leucine aminopeptidase 3
leucine aminopeptidase N
leucine aminopeptidase,
Leucyl aminopeptidase
leucyl aminopeptidase (animal)
leucyl aminopeptidase (plant)
leucyl peptidase
leucylaminopeptidase
-
leucylpeptidase
M17 family leucyl aminopeptidase metalloprotease
M17 LAP
M17 leucine amino peptidase
M17 leucine aminopeptidase
M42 aminopeptidase
PA2939
PaAP
PepA
peptidase S
PfLAP
PH1527
PhTET2
PtLAP
PVX_118180
sperm-leucylaminopeptidase
-
TbLAP1
TM0042
additional information
LAP-A
stress-induced acidic LAPs, LAP-A exhibits chaperone activity
M17 family leucyl aminopeptidase metalloprotease
-
additional information
the enzyme belongs to the M17 family leucine aminopeptidases
additional information
-
tick midgut leucine aminopeptidase belongs to the clan MF, M17 cytosolic aminopeptidase family
additional information
-
tick midgut leucine aminopeptidase belongs to the clan MF, M17 cytosolic aminopeptidase family
additional information
-
enzyme belongs to the peptidase family M17, enzyme belongs to the metallopeptidase family M1
additional information
-
the multifunctional enzyme belongs to the M1 aminopeptidase family
additional information
-
the enzyme belongs to the peptidase family M17
additional information
the enzyme belongs to the M17 family leucine aminopeptidases
additional information
the enzyme belongs to the M17 leucine peptidase family
additional information
-
the enzyme belongs to the M17 leucine peptidase family
additional information
-
the enzyme belongs to the M17 family leucine aminopeptidases
additional information
-
the enzyme belongs to the M17 family leucine aminopeptidases
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
-
-
-
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
mechanism
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
mechanism
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
catalytic residues are Arg431 and Lys354
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
exopeptidase
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly or Trp, no activity when Xaa is Asp
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly or Tyr, no activity when Xaa is Asp
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly, no activity when Xaa is Asp
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
molecular modelling of enzyme-substrate binding
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
prefers leucine and methionine as N-terminal amino acids
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
reaction mechanism, active site structure, conformation models using crystal structure
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
Lys528 is located near the entrance of the substrate pocket and is important for maximal activity by maintaining the appropriate structure of the substrate binding pocket
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
endopeptidase
-
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
endopeptidase
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
solid-state structure and structure of hydrated intermediates of the active site, catalytic mechanism, overview
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
substrate-enzyme complex structure, molecular modeling
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of peptide bond
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PATHWAY SOURCE
PATHWAYS
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CAS REGISTRY NUMBER
COMMENTARY
9001-61-0
-
90119-07-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(S)-2-amino-N-(6-(dimethylamino)pyridin-3-yl)-4-methylpentanamide + H2O
?
-
-
-
-
?
4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate + H2O
L-leucine + 4-[(E)-2-(4-aminophenyl)ethenyl-3-cyano-5,5-dimethylfuran-2(5H)-ylidene]propanedinitrile
-
-
-
-
?
Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
18.7% of activity towards Leu-4-nitroanilide
-
-
?
alanyl-7-amido-4-methylcoumarin + H2O
alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
angiotensin II + H2O
Asp + angiotensin III
-
i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
-
?
Arg-4-nitroanilide + H2O
L-arginine + 4-nitroaniline
-
52.5% of activity towards Leu-4-nitroanilide
-
-
?
Arg-Ala-Arg + H2O
Arg + Ala-Arg
-
Ala-p-nitroanilide is used as substrate with very low turnover rate
in the assay hydrolysis of Ala-p-nitroanilide is monitored
-
?
Arg-Ser-Arg + H2O
Arg + Ser-Arg
-
Ala-p-nitroanilide is used as substrate with very low turnover rate
in the assay hydrolysis of Ala-p-nitroanilide is monitored
-
?
DL-alpha-amino-acid amide + H2O
D-alpha-amino acid amide + L-alpha-amino acid
-
-
-
-
?
Gly-7-amido-4-methylcoumarin + H2O
glycine + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
-
?
glycine-4-methylcoumaryl-7-amide + H2O
glycine + 7-amino-4-methylcoumarin
-
3.9% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
hArg-7-amido-4-methylcoumarin + H2O
hArg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Ala-7-amido-4-trifluoro-methylcoumarin + H2O
L-Ala + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarine
-
-
-
?
L-Arg 7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
G4LVV4, G4VFK1
moderate activity
-
-
?
L-Arg-7-amido-4-trifluoro-methylcoumarin + H2O
L-Arg + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
L-arginine-4-nitroanilide + H2O
L-arginine + 4-nitroaniline
-
16.5% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-isoleucine + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
-
?
L-Leu-4-(phenylazo)phenylamide + H2O
L-Leu + 4-(phenylazo)phenylamine
-
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Leu
-
-
-
-
?
L-Leu-7-amido-4-trifluoro-methylcoumarin + H2O
L-Leu + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
L-lysyl-7-amido-4-methylcoumarin + H2O
L-lysine + 7-amino-4-methylcoumarin
ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin
-
-
?
L-Met 7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
G4LVV4, G4VFK1
moderate activity
-
-
?
L-methionyl-7-amido-4-methylcoumarin + H2O
L-methionine + 7-amino-4-methylcoumarin
high activity
-
-
?
L-Phe 7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
G4LVV4, G4VFK1
low activity
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarine
-
-
-
?
L-proline-4-methylcoumaryl-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
1.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-proline-4-methylcoumaryl-7-amide + H2O
L-proline + 7-amino-4-methylcoumarine
-
-
-
?
L-Thr 7-amido-4-methylcoumarin + H2O
L-Thr + 7-amino-4-methylcoumarin
G4LVV4, G4VFK1
low activity
-
-
?
L-Tyr 7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
G4LVV4, G4VFK1
lower activity
-
-
?
L-Tyr-7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
6.4% activity compared to L-Leu-7-amido-4-methylcoumarin
-
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
8.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
L-tyrosine + 7-amino-4-methylcoumarine
20% activity compared to L-leucyl-4-methylcoumaryl-7-amide
-
-
?
L-Val-7-amido-4-methylcoumarin + H2O
L-Val + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
-
?
L-valine-4-methylcoumaryl-7-amide + H2O
L-valine + 7-amino-4-methylcoumarin
-
2.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methyl-coumarin
-
-
-
-
?
Leu-4-trifluoroomethylcoumarin-7-amide + H2O
Leu + 7-amino-4-trifluoromethylcoumarin
-
-
-
-
?
Leu-Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu + H2O
Leu + Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu
-
-
-
-
?
Leu-Gly-Tyr-Leu + H2O
Leu + Gly + Tyr + Leu
-
synthetic peptide substrate, successive release of terminal amino acids with descending activity
-
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
? + Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
-
-
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
L-leucine + Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
trimming of further model epitope precursors to antigenic peptides are also tested
-
-
?
leucyl-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
-
-
-
?
Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
41.9% of activity towards Leu-4-nitroanilide
-
-
?
Met-4-methylcoumaryl-7-amide + H2O
Met + 7-amino-4-methyl-coumarin
-
-
-
-
?
N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
-
?
N-(6-methoxy-2-methylpyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
-
?
Phe-4-methylcoumaryl-7-amide + H2O
Phe + 7-amino-4-methyl-coumarin
-
-
-
-
?
Phg-7-amido-4-methylcoumarin + H2O
Phg + 7-amino-4-methylcoumarin
-
-
-
-
?
Pro-4-nitroanilide + H2O
Pro + 4-nitroaniline
-
30% of the activity with Leu-4-nitroanilide
-
-
?
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
-
-
-
?
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
-
-
-
?
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
-
42% of the activity with Leu-4-nitroanilide
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
74.5% activity compared to Leu-4-nitroanilide
-
-
?
Cys-Gly + H2O
Cys + Gly
the enzyme plays a role in glutathione turnover
-
-
?
Cys-Gly + H2O
Cys + Gly
only hydrolyzed by the Mn2+-Zn2+-enzyme, not by the Zn2+-Zn2+-enzyme
-
-
?
cysteinylglycine + H2O
cysteine + glycine
involved in turnover of glutathione
-
?
cysteinylglycine + H2O
cysteine + glycine
-
involved in the metabolism of glutathione and glutathione S-conjugates, major cysteinylglycine-hydrolysing activity
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O
Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
-
ACTH-(6-9)-PGP peptide, proteolysis of the ACTH-(6-9)-PGP is mainly defined by aminopeptidases, the basic metabolite is Trp-Pro-Gly-Pro. After 180 min, Arg-Trp-Pro-Gly-Pro and Trp-Pro-Gly-Pro are generated from Phe-Arg-Trp-Pro-Gly-Pro, and phenylalalnine cleavage proceeds much less efficiently than further cleavage of arginine
-
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O
Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
-
ACTH-(6-9)-PGP peptide, proteolysis of the ACTH-(6-9)-PGP is mainly defined by aminopeptidases, the basic metabolite is Trp-Pro-Gly-Pro. After 180 min, Arg-Trp-Pro-Gly-Pro and Trp-Pro-Gly-Pro are generated from Phe-Arg-Trp-Pro-Gly-Pro, and phenylalalnine cleavage proceeds much less efficiently than further cleavage of arginine
-
-
?
L-Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
10.8% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
10.8% of the activity compared to Leu-4-nitroanilide
-
-
?
L-alanine-4-methylcoumarin-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-alanine-4-methylcoumarin-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
32.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
20.5% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
best substrate with L-Leu at N-terminal end
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
no hydrolysis of D-Leu
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
best substrate with L-Leu at N-terminal end
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
influence of amino acid in penultimate position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
leucyl-dipeptides
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
34.7% activity compared to L-Leu-7-amido-4-methylcoumarin
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
62.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarine
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarine
about 2% activity compared to L-leucyl-4-methylcoumaryl-7-amide
-
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
low activity
-
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin
-
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
-
70% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-glutamate 4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-glutamate 4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-isoleucine 4-nitroanilide + H2O
L-isoleucine + 4-nitroaniline
14.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-isoleucine 4-nitroanilide + H2O
L-isoleucine + 4-nitroaniline
14.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
G4LVV4, G4VFK1
best substrate
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
G4LVV4, G4VFK1
fluorogenic substrate assay
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
100% activity, LAP protein demonstrates preferential substrate specificity for Leu-7-amido-4-methylcoumarin
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
very slow hydrolysis
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
substituted anilides, e.g. o-, m- or p-aminobenzenesulfonic acid, o- or m-anisidine, m- or p-aminobenzenesulfonylfluoride
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
Leu substituted by Ala, Gly, Lys
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
very slow hydrolysis
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine 4-nitroanilide + H2O
leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-methylcoumarin-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-4-methylcoumarin-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
best synthetic substrate
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
moderate activity
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
ERAP1 preferentially hydrolyzes Leu-aminoacyl-aminomethylcoumarin
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
preferred substrate
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
best substrate
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
best substrate
-
-
?
L-Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide
-
-
?
L-Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
-
?
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
24.5% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
24.5% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
no activity with Z-Met-7-amido-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
no activity with Z-Met-7-amido-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
26.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
about 25% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
about 25% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
26.3% of the activity compared to Leu-4-nitroanilide
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
-
?
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
29.6% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
29.6% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
low activity
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
low activity
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
-
38.8% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
-
38.8% activity in comparison to L-leucine-4-nitroanilide
-
-
?
L-Pro-4-nitroanilide + H2O
L-proline + 4-nitroaniline
very low activity
-
-
?
L-Pro-4-nitroanilide + H2O
L-proline + 4-nitroaniline
very low activity
-
-
?
L-proline-4-methylcoumarin-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
-
?
L-proline-4-methylcoumarin-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
7.4% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
-
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
Leu replaced by Met, Phe
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
-
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
-
-
-
?
Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
-
Leu replaced by Arg
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
-
Leu replaced by Met
-
-
?
Leu-Gly + H2O
Leu + Gly
substrate of the Mn2+-Zn2+-enzyme and the Zn2+-Zn2+-enzyme
-
-
?