Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-2-amino-N-(6-(dimethylamino)pyridin-3-yl)-4-methylpentanamide + H2O
?
-
-
-
?
4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate + H2O
L-leucine + 4-[(E)-2-(4-aminophenyl)ethenyl-3-cyano-5,5-dimethylfuran-2(5H)-ylidene]propanedinitrile
-
-
-
?
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
18.7% of activity towards Leu-4-nitroanilide
-
?
Ala-Ala-Ala-Ala
?
-
-
-
?
alaninehydrazine
?
-
-
-
?
alanyl-7-amido-4-methylcoumarin + H2O
alanine + 7-amino-4-methylcoumarin
-
-
-
?
amino acid amides + H2O
amino acid + NH3
angiotensin II + H2O
angiotensin IV + ?
-
-
through angiotensin III
?
angiotensin II + H2O
Asp + angiotensin III
-
i.e. Asp-Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe
?
Arg 4-nitroanilide + H2O
Arg + 4-nitroaniline
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
Arg-4-nitroanilide + H2O
L-arginine + 4-nitroaniline
-
52.5% of activity towards Leu-4-nitroanilide
-
?
Arg-Ala-Arg + H2O
Arg + Ala-Arg
-
Ala-p-nitroanilide is used as substrate with very low turnover rate
in the assay hydrolysis of Ala-p-nitroanilide is monitored
?
Arg-Gly-Arg + H2O
Arg + Gly-Arg
Arg-Gly-Asp + H2O
Arg + Gly-Asp
Arg-Gly-Gly + H2O
Arg + Gly-Gly
Arg-Gly-Leu + H2O
Arg + Gly-Leu
Arg-Gly-Phe + H2O
Arg + Gly-Phe
Arg-Gly-Ser + H2O
Arg + Gly-Ser
Arg-Ser-Arg + H2O
Arg + Ser-Arg
-
Ala-p-nitroanilide is used as substrate with very low turnover rate
in the assay hydrolysis of Ala-p-nitroanilide is monitored
?
Asn-Leu + H2O
Asn + Leu
-
-
?
Asp-Gln + H2O
Asp + Gln
-
-
?
Asp-His + H2O
Asp + His
-
-
?
Asp-Ile-Gly-Gly + H2O
Asp + Ile-Gly-Gly
-
-
?
Asp-Leu + H2O
Asp + Leu
-
-
?
Asp-Leu-Gly + H2O
Asp + Leu-Gly
-
-
?
Asp-Leu-Lys + H2O
Asp + Leu-Lys
-
-
?
Asp-Phe + H2O
Asp + Phe
-
-
?
Asp-Tyr + H2O
Asp + Tyr
-
-
?
benzyl-L-cysteinamide + H2O
?
bimane-S-cysteinylglycine + H2O
?
-
-
-
?
Cys-Leu + H2O
Cys + Leu
-
-
?
cysteinyl-bis-glycine + H2O
cysteine + glycine
-
-
-
?
cysteinylglycine + H2O
cysteine + glycine
DL-alpha-amino-acid amide + H2O
D-alpha-amino acid amide + L-alpha-amino acid
-
-
-
?
dynorphin A + H2O
?
-
-
-
?
Gln-Leu + H2O
Gln + Leu
-
-
?
Glu-Leu + H2O
Glu + Leu
-
-
?
Gly-7-amido-4-methylcoumarin + H2O
glycine + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
?
Gly-Val + H2O
Gly + Val
-
low activity
-
?
glycine-4-methylcoumaryl-7-amide + H2O
glycine + 7-amino-4-methylcoumarin
-
3.9% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
?
hArg-7-amido-4-methylcoumarin + H2O
hArg + 7-amino-4-methylcoumarin
-
-
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O
Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
Ile-Leu + H2O
Ile + Leu
-
-
?
kallidin + H2O
Leu + bradykinin
-
i.e. LRPPGFSPFR
i.e. RPPGFSPFR
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
L-Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
L-Ala-7-amido-4-trifluoro-methylcoumarin + H2O
L-Ala + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
L-Ala-L-Leu + H2O
L-Ala + L-Leu
-
-
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
L-alanine-4-methylcoumarin-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarine
-
-
?
L-alanyl-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
L-amino acid-peptide + H2O
amino acid + peptide
L-Arg 7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
moderate activity
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
L-Arg-7-amido-4-trifluoro-methylcoumarin + H2O
L-Arg + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarine
L-arginine-4-nitroanilide + H2O
L-arginine + 4-nitroaniline
-
16.5% activity in comparison to L-leucine-4-nitroanilide
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
L-glutamate 4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
L-His-L-Leu + H2O
L-His + L-Leu
-
-
-
?
L-Ile-4-nitroanilide + H2O
L-Ile + 4-nitroaniline
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
L-Ile-7-amido-4-methylcoumarin + H2O
L-isoleucine + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
?
L-isoleucine 4-nitroanilide + H2O
L-isoleucine + 4-nitroaniline
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
L-Leu beta-naphthylamide + H2O
L-Leu + beta-naphthylamine
-
-
-
?
L-Leu methyl ester
?
-
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
L-Leu-4-(phenylazo)phenylamide + H2O
L-Leu + 4-(phenylazo)phenylamine
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
L-Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-Leu-7-amido-4-methylcoumarin + H2O
7-amino-4-methylcoumarin + L-Leu
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
L-Leu-7-amido-4-trifluoro-methylcoumarin + H2O
L-Leu + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
L-Leu-benzyl ester + H2O
L-Leu + benzylalcohol
-
-
-
?
L-Leu-Gly + H2O
L-Leu + Gly
L-Leu-hydrazide
?
-
-
-
?
L-Leu-L-Arg + H2O
L-Leu + L-Arg
-
-
-
?
L-Leu-L-Asn + H2O
L-Leu + L-Asn
-
-
-
?
L-Leu-L-Asp + H2O
L-Leu + L-Asp
-
low activity
-
?
L-Leu-L-Leu + H2O
L-Leu + L-Leu
-
-
-
?
L-Leu-L-Met + H2O
L-Leu + L-Met
-
-
-
?
L-Leu-L-Phe + H2O
L-Leu + L-Phe
-
-
-
?
L-Leu-L-Ser + H2O
L-Leu + L-Ser
-
-
-
?
L-Leu-L-Tyr + H2O
L-Leu + L-Tyr
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
L-Leu-phenylazophenylamide
?
-
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-leucine 4-nitroanilide + H2O
leucine + 4-nitroaniline
L-leucine amide + H2O
L-leucine + NH3
L-leucine hydrazide + H2O
L-leucine + azide
L-leucine-4-methylcoumarin-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucine-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
-
?
L-leucine-p-nitroanilide + H2O
L-leucine + p-nitroaniline
L-leucinhydrazide + H2O
L-leucine + H2NNH2
-
-
-
?
L-leucyl-2-naphthylamide + H2O
L-leucine + 2-naphthylamine
L-leucyl-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
L-leucylamide + H2O
L-leucine + NH3
-
-
-
?
L-leucylglycine + H2O
L-leucine + glycine
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
-
-
?
L-Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
L-lysyl-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
-
-
?
L-lysyl-7-amido-4-methylcoumarin + H2O
L-lysine + 7-amino-4-methylcoumarin
ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin
-
?
L-Met 7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
moderate activity
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
L-Met-Gly + H2O
L-Met + Gly
-
-
?
L-Met-L-Leu + H2O
L-Met + L-Leu
-
-
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
L-methionyl-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
-
-
?
L-methionyl-7-amido-4-methylcoumarin + H2O
L-methionine + 7-amino-4-methylcoumarin
high activity
-
?
L-Phe 7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
low activity
-
?
L-Phe-4-nitroanilide + H2O
L-Phe + 4-nitroaniline
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
L-Phe-L-Leu + H2O
L-Phe + L-Leu
-
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarine
-
-
?
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
L-Pro-4-nitroanilide + H2O
L-Pro + 4-nitroaniline
-
-
?
L-Pro-4-nitroanilide + H2O
L-proline + 4-nitroaniline
L-Pro-L-Leu + H2O
L-Pro + L-Leu
-
-
-
?
L-proline-4-methylcoumarin-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
L-proline-4-methylcoumaryl-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
1.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
?
L-proline-4-methylcoumaryl-7-amide + H2O
L-proline + 7-amino-4-methylcoumarine
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O
L-proline + 7-amino-4-methylcoumarin
L-Ser-Gly + H2O
L-Ser + Gly
-
-
?
L-Thr 7-amido-4-methylcoumarin + H2O
L-Thr + 7-amino-4-methylcoumarin
low activity
-
?
L-Thr-4-nitroanilide + H2O
L-Thr + 4-nitroaniline
-
-
?
L-Thr-L-Leu + H2O
L-Thr + L-Leu
-
-
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
L-Trp-L-Leu + H2O
L-Trp + L-Leu
-
-
-
?
L-Tyr 7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
lower activity
-
?
L-Tyr-7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
6.4% activity compared to L-Leu-7-amido-4-methylcoumarin
-
?
L-Tyr-L-Leu + H2O
L-Tyr + L-Leu
-
-
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
8.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O
L-tyrosine + 7-amino-4-methylcoumarine
20% activity compared to L-leucyl-4-methylcoumaryl-7-amide
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
L-Val-7-amido-4-methylcoumarin + H2O
L-Val + 7-amino-4-methylcoumarin
enzyme shows only weak activity with this substrate
-
?
L-Val-L-Leu + H2O
L-Val + L-Leu
-
-
-
?
L-valine-4-methylcoumaryl-7-amide + H2O
L-valine + 7-amino-4-methylcoumarin
-
2.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
Leu-2-naphthylamide + H2O
Leu + 2-naphthylamine
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methyl-coumarin
-
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
Leu-4-trifluoroomethylcoumarin-7-amide + H2O
Leu + 7-amino-4-trifluoromethylcoumarin
-
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
Leu + 7-amino-4-methylcoumarin
-
-
-
?
Leu-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
Leu-Arg-Pro-Gly + H2O
Leu + Arg-Pro-Gly
-
-
-
?
Leu-Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu + H2O
Leu + Gly-Arg-Ser-Gly-Gly-Asp-Ile-Ile-Lys-Lys-Met-Gln-Thr-Leu
-
-
-
?
Leu-Gly-Gly + H2O
Leu + Gly-Gly
Leu-Gly-Tyr-Leu + H2O
Leu + Gly + Tyr + Leu
-
synthetic peptide substrate, successive release of terminal amino acids with descending activity
-
?
Leu-Leu-Leu + H2O
Leu + Leu-Leu
Leu-p-nitroanilide + H2O
Leu + p-nitroaniline
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
? + Ile-Asn-Phe-Glu-Lys-Leu
-
-
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
? + Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
-
-
?
Leu-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu + H2O
L-leucine + Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu
-
trimming of further model epitope precursors to antigenic peptides are also tested
-
?
Leu-systemin + H2O
Leu + systemin
-
LAP-A1, only in vitro
-
?
leucyl-7-amido-4-methylcoumarin + H2O
leucine + 7-amino-4-methylcoumarin
-
-
?
leukotriene D4 + H2O
?
-
low activity
-
?
Lys 4-nitroanilide + H2O
Lys + 4-nitroaniline
Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
41.9% of activity towards Leu-4-nitroanilide
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
Met 4-nitroanilide + H2O
Met + 4-nitroaniline
Met-4-methylcoumaryl-7-amide + H2O
Met + 7-amino-4-methyl-coumarin
-
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
Met-Glu-His-Phe-Pro-Gly-Pro + H2O
?
N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
?
N-(6-methoxy-2-methylpyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
?
N-(6-methoxypyridin-3-yl)-(S)-2-amino-4-methylpentanamide + H2O
?
-
-
-
?
N-(tert-butoxycarbonyl)-L-leucine + H2O
?
-
-
-
?
N-succinyl-L-Leu-4-nitroanilide + H2O
N-succinyl-L-Leu + 4-nitroaniline
Neurokinin A + H2O
?
-
-
-
?
Neuromedin B + H2O
?
-
-
-
?
Phe 4-nitroanilide + H2O
Phe + 4-nitroaniline
-
-
?
Phe-4-methylcoumaryl-7-amide + H2O
Phe + 7-amino-4-methyl-coumarin
-
-
-
?
Phe-4-methylcoumaryl-7-amide + H2O
Phe + 7-amino-4-methylcoumarin
Phg-7-amido-4-methylcoumarin + H2O
Phg + 7-amino-4-methylcoumarin
-
-
-
?
phosphatidylinositol-dependent kinase-1 + H2O
?
Pro-4-methylcoumaryl-7-amide + H2O
Pro + 7-amino-4-methylcoumarin
Pro-4-nitroanilide + H2O
Pro + 4-nitroaniline
-
30% of the activity with Leu-4-nitroanilide
-
?
S-nitrosocysteinylglycine + H2O
S-nitrosocysteine + glycine
-
-
-
?
Ser-Gly + H2O
Ser + Gly
-
-
?
Ser-Leu + H2O
Ser + Leu
-
-
?
additional information
?
-
Ala-4-methylcoumaryl-7-amide + H2O

Ala + 7-amino-4-methylcoumarin
-
-
?
Ala-4-methylcoumaryl-7-amide + H2O
Ala + 7-amino-4-methylcoumarin
-
-
?
Ala-4-nitroanilide + H2O

Ala + 4-nitroaniline
-
42% of the activity with Leu-4-nitroanilide
-
?
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
-
-
-
?
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
-
-
?
Ala-Gly + H2O

Ala + Gly
-
low activity
-
?
Ala-Gly + H2O
Ala + Gly
-
-
-
?
Ala-Gly + H2O
Ala + Gly
-
low activity
-
?
Ala-Leu + H2O

Ala + Leu
-
high activity
-
?
Ala-Leu + H2O
Ala + Leu
-
-
?
Ala-Leu + H2O
Ala + Leu
-
high activity
-
?
Ala-Leu + H2O
Ala + Leu
-
high activity
-
?
amino acid amides + H2O

amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
-
-
?
amino acid amides + H2O
amino acid + NH3
-
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
-
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
slow hydrolysis: Tyr, Phe
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
D,L-norleucine
-
?
amino acid amides + H2O
amino acid + NH3
trout
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
amino acid amides + H2O
amino acid + NH3
-
fast hydrolysis: L-Leu
-
?
Arg 4-nitroanilide + H2O

Arg + 4-nitroaniline
-
-
?
Arg 4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
?
Arg-4-nitroanilide + H2O

Arg + 4-nitroaniline
74.5% activity compared to Leu-4-nitroanilide
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
-
-
?
Arg-Ala + H2O

Arg + Ala
-
-
-
?
Arg-Ala + H2O
Arg + Ala
-
-
-
?
Arg-Ala + H2O
Arg + Ala
-
-
-
?
Arg-Glu + H2O

Arg + Glu
-
low activity
-
?
Arg-Glu + H2O
Arg + Glu
-
low activity
-
?
Arg-Glu + H2O
Arg + Glu
-
low activity
-
?
Arg-Gly + H2O

Arg + Gly
-
-
-
?
Arg-Gly + H2O
Arg + Gly
-
low activity
-
?
Arg-Gly + H2O
Arg + Gly
-
-
-
?
Arg-Gly + H2O
Arg + Gly
-
low activity
-
?
Arg-Gly + H2O
Arg + Gly
-
-
-
?
Arg-Gly + H2O
Arg + Gly
-
low activity
-
?
Arg-Gly-Arg + H2O

Arg + Gly-Arg
-
low activity
-
?
Arg-Gly-Arg + H2O
Arg + Gly-Arg
-
low activity
-
?
Arg-Gly-Arg + H2O
Arg + Gly-Arg
-
low activity
-
?
Arg-Gly-Asp + H2O

Arg + Gly-Asp
-
-
-
?
Arg-Gly-Asp + H2O
Arg + Gly-Asp
-
-
-
?
Arg-Gly-Asp + H2O
Arg + Gly-Asp
-
-
-
?
Arg-Gly-Gly + H2O

Arg + Gly-Gly
-
-
-
?
Arg-Gly-Gly + H2O
Arg + Gly-Gly
-
-
-
?
Arg-Gly-Gly + H2O
Arg + Gly-Gly
-
-
-
?
Arg-Gly-Leu + H2O

Arg + Gly-Leu
-
-
-
?
Arg-Gly-Leu + H2O
Arg + Gly-Leu
-
-
-
?
Arg-Gly-Leu + H2O
Arg + Gly-Leu
-
-
-
?
Arg-Gly-Phe + H2O

Arg + Gly-Phe
-
-
-
?
Arg-Gly-Phe + H2O
Arg + Gly-Phe
-
-
-
?
Arg-Gly-Phe + H2O
Arg + Gly-Phe
-
-
-
?
Arg-Gly-Ser + H2O

Arg + Gly-Ser
-
-
-
?
Arg-Gly-Ser + H2O
Arg + Gly-Ser
-
-
-
?
Arg-Gly-Ser + H2O
Arg + Gly-Ser
-
-
-
?
Arg-Ile + H2O

Arg + Ile
-
-
-
?
Arg-Ile + H2O
Arg + Ile
-
-
-
?
Arg-Ile + H2O
Arg + Ile
-
-
-
?
Arg-Leu + H2O

Arg + Leu
-
high activity
-
?
Arg-Leu + H2O
Arg + Leu
-
high activity
-
?
Arg-Leu + H2O
Arg + Leu
-
high activity
-
?
Arg-Phe + H2O

Arg + Phe
-
-
-
?
Arg-Phe + H2O
Arg + Phe
-
-
-
?
Arg-Phe + H2O
Arg + Phe
-
-
-
?
Arg-Val + H2O

Arg + Val
-
-
-
?
Arg-Val + H2O
Arg + Val
-
-
-
?
Arg-Val + H2O
Arg + Val
-
-
-
?
benzyl-L-cysteinamide + H2O

?
-
-
-
?
benzyl-L-cysteinamide + H2O
?
-
-
-
?
Cys-Gly + H2O

Cys + Gly
-
-
?
Cys-Gly + H2O
Cys + Gly
-
-
-
?
Cys-Gly + H2O
Cys + Gly
the enzyme plays a role in glutathione turnover
-
?
Cys-Gly + H2O
Cys + Gly
only hydrolyzed by the Mn2+-Zn2+-enzyme, not by the Zn2+-Zn2+-enzyme
-
?
Cys-Gly + H2O
Cys + Gly
-
low activity
-
?
Cys-Gly + H2O
Cys + Gly
-
low activity
-
?
cysteinylglycine + H2O

cysteine + glycine
involved in turnover of glutathione
-
?
cysteinylglycine + H2O
cysteine + glycine
-
-
-
?
cysteinylglycine + H2O
cysteine + glycine
-
involved in the metabolism of glutathione and glutathione S-conjugates, major cysteinylglycine-hydrolysing activity
-
?
Gly-Leu + H2O

Gly + Leu
-
low activity
-
?
Gly-Leu + H2O
Gly + Leu
-
low activity
-
?
Gly-Leu + H2O
Gly + Leu
-
low activity
-
?
Gly-Phe + H2O

Gly + Phe
-
low activity
-
?
Gly-Phe + H2O
Gly + Phe
-
-
-
?
Gly-Phe + H2O
Gly + Phe
-
-
-
?
Gly-Phe + H2O
Gly + Phe
-
-
-
?
Gly-Trp + H2O

Gly + Trp
-
low activity
-
?
Gly-Trp + H2O
Gly + Trp
-
low activity
-
?
Gly-Tyr + H2O

Gly + Tyr
-
low activity
-
?
Gly-Tyr + H2O
Gly + Tyr
-
low activity
-
?
Gly-Tyr + H2O
Gly + Tyr
-
low activity
-
?
His-Gly + H2O

His + Gly
-
-
-
?
His-Gly + H2O
His + Gly
-
-
-
?
His-Gly + H2O
His + Gly
-
-
-
?
His-Leu + H2O

His + Leu
-
low activity
-
?
His-Leu + H2O
His + Leu
-
-
?
His-Leu + H2O
His + Leu
-
-
-
?
His-Leu + H2O
His + Leu
-
-
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O

Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
-
ACTH-(6-9)-PGP peptide, proteolysis of the ACTH-(6-9)-PGP is mainly defined by aminopeptidases, the basic metabolite is Trp-Pro-Gly-Pro. After 180 min, Arg-Trp-Pro-Gly-Pro and Trp-Pro-Gly-Pro are generated from Phe-Arg-Trp-Pro-Gly-Pro, and phenylalalnine cleavage proceeds much less efficiently than further cleavage of arginine
-
?
His-Phe-Arg-Trp-Pro-Gly-Pro + H2O
Trp-Pro-Gly-Pro + Arg-Trp-Pro-Gly-Pro
-
ACTH-(6-9)-PGP peptide, proteolysis of the ACTH-(6-9)-PGP is mainly defined by aminopeptidases, the basic metabolite is Trp-Pro-Gly-Pro. After 180 min, Arg-Trp-Pro-Gly-Pro and Trp-Pro-Gly-Pro are generated from Phe-Arg-Trp-Pro-Gly-Pro, and phenylalalnine cleavage proceeds much less efficiently than further cleavage of arginine
-
?
L-Ala-4-nitroanilide + H2O

L-Ala + 4-nitroaniline
-
-
?
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
-
-
-
?
L-Ala-4-nitroanilide + H2O

L-alanine + 4-nitroaniline
-
-
?
L-Ala-4-nitroanilide + H2O
L-alanine + 4-nitroaniline
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O

L-Ala + 7-amino-4-methylcoumarin
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
low activity
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
-
-
-
?
L-alanine 4-nitroanilide + H2O

L-alanine + 4-nitroaniline
10.8% of the activity compared to Leu-4-nitroanilide
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
?
L-alanine 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
10.8% of the activity compared to Leu-4-nitroanilide
-
?
L-alanine-4-methylcoumarin-7-amide + H2O

L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanine-4-methylcoumarin-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O

L-alanine + 7-amino-4-methylcoumarin
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
32.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O

L-alanine + 7-amino-4-methylcoumarin
-
20.5% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
?
L-alanyl-7-amido-4-methylcoumarin + H2O
L-alanine + 7-amino-4-methylcoumarin
-
-
-
?
L-amino acid-peptide + H2O

amino acid + peptide
-
best substrate with L-Leu at N-terminal end
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
e.g. Leu-Leu
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
His-Lys
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
no hydrolysis of D-Leu
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Phe-Leu
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Ser-Leu
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
e.g. Leu-Leu
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Arg
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Met
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Ala-Ala-Ala-Ala
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
best substrate with L-Leu at N-terminal end
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
influence of amino acid in penultimate position
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Gly-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
trout
-
Leu-Gly
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
leucyl-dipeptides
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Leu-Met
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
Pro-Phe-Lys
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
free N-terminal alpha-amino group required
-
?
L-amino acid-peptide + H2O
amino acid + peptide
-
fast hydrolysis of hydrophobic amino acids in N-terminal position
-
?
L-Arg-4-nitroanilide + H2O

L-Arg + 4-nitroaniline
-
-
-
?
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
-
pre-LAP
-
?
L-Arg-7-amido-4-methylcoumarin + H2O

L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
34.7% activity compared to L-Leu-7-amido-4-methylcoumarin
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O

L-arginine + 7-amino-4-methylcoumarin
-
-
-
?
L-arginine-4-methylcoumarin-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O

L-arginine + 7-amino-4-methylcoumarin
-
62.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarin
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O

L-arginine + 7-amino-4-methylcoumarine
-
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O
L-arginine + 7-amino-4-methylcoumarine
about 2% activity compared to L-leucyl-4-methylcoumaryl-7-amide
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O

L-arginine + 7-amino-4-methylcoumarin
low activity
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin
-
?
L-arginyl-7-amido-4-methylcoumarin + H2O
L-arginine + 7-amino-4-methylcoumarin
-
70% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
?
L-Cys-7-amido-4-methylcoumarin + H2O

L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
-
-
?
L-glutamate 4-nitroanilide + H2O

L-glutamate + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
?
L-glutamate 4-nitroanilide + H2O
L-glutamate + 4-nitroaniline
about 20% of the activity compared to Leu-4-nitroanilide
-
?
L-Ile-4-nitroanilide + H2O

L-Ile + 4-nitroaniline
-
-
?
L-Ile-4-nitroanilide + H2O
L-Ile + 4-nitroaniline
-
-
-
?
L-Ile-7-amido-4-methylcoumarin + H2O

L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-Ile-7-amido-4-methylcoumarin + H2O
L-Ile + 7-amino-4-methylcoumarin
low activity
-
?
L-isoleucine 4-nitroanilide + H2O

L-isoleucine + 4-nitroaniline
14.3% of the activity compared to Leu-4-nitroanilide
-
?
L-isoleucine 4-nitroanilide + H2O
L-isoleucine + 4-nitroaniline
14.3% of the activity compared to Leu-4-nitroanilide
-
?
L-Leu 7-amido-4-methylcoumarin + H2O

L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best substrate
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
fluorogenic substrate assay
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu-2-naphthylamide + H2O

L-Leu + 2-naphthylamine
-
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
-
-
-
?
L-Leu-4-nitroanilide + H2O

L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
pre-LAP
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
-
-
-
?
L-Leu-4-nitroanilide + H2O

L-leucine + 4-nitroaniline
-
-
?
L-Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O

L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
best substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
best substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
best synthetic substrate
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
100% activity, LAP protein demonstrates preferential substrate specificity for Leu-7-amido-4-methylcoumarin
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
-
-
?
L-Leu-Gly + H2O

L-Leu + Gly
-
-
-
?
L-Leu-Gly + H2O
L-Leu + Gly
-
-
-
?
L-Leu-Gly + H2O
L-Leu + Gly
-
-
-
?
L-Leu-p-nitroanilide + H2O

L-Leu + p-nitroaniline
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-Leu + p-nitroaniline
-
-
?
L-Leu-p-nitroanilide + H2O

L-leucine + p-nitroaniline
-
very slow hydrolysis
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
substituted anilides, e.g. o-, m- or p-aminobenzenesulfonic acid, o- or m-anisidine, m- or p-aminobenzenesulfonylfluoride
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
Leu substituted by Ala, Gly, Lys
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
no hydrolysis
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
very slow hydrolysis
-
?
L-Leu-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
?
L-leucine 4-nitroanilide + H2O

L-leucine + 4-nitroaniline
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
?
L-leucine 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
?
L-leucine 4-nitroanilide + H2O

leucine + 4-nitroaniline
-
-
?
L-leucine 4-nitroanilide + H2O
leucine + 4-nitroaniline
-
-
?
L-leucine amide + H2O

L-leucine + NH3
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
?
L-leucine amide + H2O
L-leucine + NH3
-
-
-
?
L-leucine hydrazide + H2O

L-leucine + azide
-
-
-
?
L-leucine hydrazide + H2O
L-leucine + azide
-
-
-
?
L-leucine hydrazide + H2O
L-leucine + azide
-
-
-
?
L-leucine-4-methylcoumarin-7-amide + H2O

L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumarin-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O

L-leucine + 7-amino-4-methylcoumarin
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O

L-leucine + 7-amino-4-methylcoumarine
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
best synthetic substrate
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
L-leucine + 7-amino-4-methylcoumarine
-
-
-
?
L-leucine-4-nitroanilide + H2O

L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O

L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucine-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
?
L-leucine-p-nitroanilide + H2O

L-leucine + p-nitroaniline
-
-
-
?
L-leucine-p-nitroanilide + H2O
L-leucine + p-nitroaniline
-
-
-
?
L-leucyl-2-naphthylamide + H2O

L-leucine + 2-naphthylamine
-
-
-
?
L-leucyl-2-naphthylamide + H2O
L-leucine + 2-naphthylamine
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O

L-leucine + 7-amino-4-methylcoumarin
moderate activity
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
ERAP1 preferentially hydrolyzes Leu-aminoacyl-aminomethylcoumarin
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
preferred substrate
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
-
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
best substrate
-
?
L-leucyl-7-amido-4-methylcoumarin + H2O
L-leucine + 7-amino-4-methylcoumarin
-
best substrate
-
?
L-leucylglycine + H2O

L-leucine + glycine
-
-
-
?
L-leucylglycine + H2O
L-leucine + glycine
-
-
-
?
L-Lys-4-nitroanilide + H2O

L-lysine + 4-nitroaniline
hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide
-
?
L-Lys-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
hydrolyzes L-Lys-4-nitroanilide with efficiency comparable to that of L-Leu-4-nitroanilide
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O

L-lysine + 7-amino-4-methylcoumarin
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
?
L-lysine-4-methylcoumaryl-7-amide + H2O
L-lysine + 7-amino-4-methylcoumarin
-
-
?
L-lysine-4-nitroanilide + H2O

L-lysine + 4-nitroaniline
-
24.5% activity in comparison to L-leucine-4-nitroanilide
-
?
L-lysine-4-nitroanilide + H2O
L-lysine + 4-nitroaniline
-
24.5% activity in comparison to L-leucine-4-nitroanilide
-
?
L-Met-4-nitroanilide + H2O

L-Met + 4-nitroaniline
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
-
?
L-Met-4-nitroanilide + H2O
L-Met + 4-nitroaniline
-
pre-LAP
-
?
L-Met-7-amido-4-methylcoumarin + H2O

L-Met + 7-amino-4-methylcoumarin
-
no activity with Z-Met-7-amido-4-methylcoumarin
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
no activity with Z-Met-7-amido-4-methylcoumarin
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
-
-
?
L-methionine 4-nitroanilide + H2O

L-methionine + 4-nitroaniline
26.3% of the activity compared to Leu-4-nitroanilide
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
about 25% of the activity compared to Leu-4-nitroanilide
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
about 25% of the activity compared to Leu-4-nitroanilide
-
?
L-methionine 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
26.3% of the activity compared to Leu-4-nitroanilide
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O

L-methionine + 7-amino-4-methylcoumarin
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
?
L-methionine-4-methylcoumaryl-7-amide + H2O
L-methionine + 7-amino-4-methylcoumarin
-
-
?
L-methionine-4-nitroanilide + H2O

L-methionine + 4-nitroaniline
-
29.6% activity in comparison to L-leucine-4-nitroanilide
-
?
L-methionine-4-nitroanilide + H2O
L-methionine + 4-nitroaniline
-
29.6% activity in comparison to L-leucine-4-nitroanilide
-
?
L-Phe-7-amido-4-methylcoumarin + H2O

L-Phe + 7-amino-4-methylcoumarin
-
low activity
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
low activity
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O

L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumarin-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O

L-phenylalanine + 7-amino-4-methylcoumarin
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
L-phenylalanine + 7-amino-4-methylcoumarin
-
-
?
L-phenylalanine-4-nitroanilide + H2O

L-phenylalanine + 4-nitroaniline
-
38.8% activity in comparison to L-leucine-4-nitroanilide
-
?
L-phenylalanine-4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
-
38.8% activity in comparison to L-leucine-4-nitroanilide
-
?
L-Pro-4-nitroanilide + H2O

L-proline + 4-nitroaniline
very low activity
-
?
L-Pro-4-nitroanilide + H2O
L-proline + 4-nitroaniline
very low activity
-
?
L-proline-4-methylcoumarin-7-amide + H2O

L-proline + 7-amino-4-methylcoumarin
-
-
-
?
L-proline-4-methylcoumarin-7-amide + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O

L-proline + 7-amino-4-methylcoumarin
-
-
?
L-prolyl-7-amido-4-methylcoumarin + H2O
L-proline + 7-amino-4-methylcoumarin
-
-
-
?
L-Trp-7-amido-4-methylcoumarin + H2O

L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-Trp-7-amido-4-methylcoumarin + H2O
L-Trp + 7-amino-4-methylcoumarin
low activity
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O

L-tyrosine + 7-amino-4-methylcoumarin
-
7.4% activity compared to L-leucyl-7-amido-4-methylcoumarin
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
-
?
L-tyrosyl-7-amido-4-methylcoumarin + H2O
L-tyrosine + 7-amino-4-methylcoumarin
-
-
-
?
L-Val-4-nitroanilide + H2O

L-Val + 4-nitroaniline
-
-
?
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
-
-
-
?
Leu 4-nitroanilide + H2O

Leu + 4-nitroaniline
-
-
?
Leu 4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
?
Leu-2-naphthylamide + H2O

leucine + 2-naphthylamine
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
-
-
?
Leu-2-naphthylamide + H2O
leucine + 2-naphthylamine
-
Leu replaced by Met, Phe
-
?
Leu-4-methylcoumaryl-7-amide + H2O

Leu + 7-amino-4-methylcoumarin
-
-
?
Leu-4-methylcoumaryl-7-amide + H2O
Leu + 7-amino-4-methylcoumarin
-
-
?
Leu-4-nitroanilide + H2O

L-leucine + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
L-leucine + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O

Leu + 4-nitroaniline
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
best substrate
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline