Cloned (Comment) | Organism |
---|---|
constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein | Leishmania donovani |
constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein | Leishmania major |
constitutive single copy lap gene, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein | Leishmania amazonensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | strong inhibition | Leishmania amazonensis | |
1,10-phenanthroline | strong inhibition | Leishmania donovani | |
1,10-phenanthroline | strong inhibition | Leishmania major | |
1,7-phenanthroline | slight inhibition | Leishmania amazonensis | |
1,7-phenanthroline | slight inhibition | Leishmania donovani | |
1,7-phenanthroline | slight inhibition | Leishmania major | |
actinonin | tight-binding, competitive inhibitor | Leishmania amazonensis | |
actinonin | tight-binding, competitive inhibitor | Leishmania donovani | |
actinonin | tight-binding, competitive inhibitor | Leishmania major | |
apstatin | strong inhibition | Leishmania amazonensis | |
apstatin | strong inhibition | Leishmania donovani | |
apstatin | strong inhibition | Leishmania major | |
arphamenine A | non-tight-binding, competitive inhibitor | Leishmania amazonensis | |
arphamenine A | non-tight-binding, competitive inhibitor | Leishmania donovani | |
arphamenine A | non-tight-binding, competitive inhibitor | Leishmania major | |
bestatin | strong inhibition | Leishmania amazonensis | |
bestatin | strong inhibition | Leishmania donovani | |
bestatin | strong inhibition | Leishmania major | |
Ca2+ | complete inhibition at 0.1-10 mM | Leishmania amazonensis | |
Cu2+ | 93% inhibition at 1 mM | Leishmania amazonensis | |
DL-thiorphan | slight inhibition at 0.1 mM | Leishmania amazonensis | |
DL-thiorphan | slight inhibition at 0.1 mM | Leishmania donovani | |
DL-thiorphan | slight inhibition at 0.1 mM | Leishmania major | |
EDTA | strong inhibition | Leishmania amazonensis | |
EDTA | strong inhibition | Leishmania donovani | |
EDTA | strong inhibition | Leishmania major | |
EGTA | - |
Leishmania amazonensis | |
EGTA | - |
Leishmania donovani | |
EGTA | - |
Leishmania major | |
Fe2+ | complete inhibition at 1-10 mM | Leishmania amazonensis | |
GSH | slight inhibition | Leishmania donovani | |
GSH | slight inhibition | Leishmania major | |
L-Leucinol | non-tight-binding, competitive inhibitor | Leishmania amazonensis | |
L-Leucinol | non-tight-binding, competitive inhibitor | Leishmania donovani | |
L-Leucinol | non-tight-binding, competitive inhibitor | Leishmania major | |
additional information | no inhibition by DTT and GSH, inhibition mechanisms | Leishmania amazonensis | |
additional information | no inhibition by DTT, inhibition mechanisms | Leishmania donovani | |
additional information | no inhibition by DTT, inhibition mechanisms | Leishmania major | |
phosphoramidon | slight inhibition at 0.1 mM | Leishmania amazonensis | |
phosphoramidon | slight inhibition at 0.1 mM | Leishmania donovani | |
phosphoramidon | slight inhibition at 0.1 mM | Leishmania major | |
puromycin | slight inhibition | Leishmania amazonensis | |
puromycin | slight inhibition | Leishmania donovani | |
puromycin | slight inhibition | Leishmania major | |
Zn2+ | inhibitory at 10 mM, slightly activating at 1 mM | Leishmania amazonensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.044 | - |
L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.054 | - |
L-Ile-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.06 | - |
L-Ile-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.062 | - |
L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.072 | - |
L-Trp-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.077 | - |
L-Ile-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.104 | - |
L-Trp-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.105 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.118 | - |
L-Trp-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.137 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.154 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.167 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.189 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.229 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.392 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.408 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.442 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Leishmania amazonensis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates best at 1 mM | Leishmania amazonensis | |
Mg2+ | activates at 1-10 mM | Leishmania amazonensis | |
Mn2+ | activates at 0.1-1 mM | Leishmania amazonensis | |
Ni2+ | activates at 1-10 mM | Leishmania amazonensis | |
Zn2+ | bound to the enzyme as native cofactor | Leishmania amazonensis | |
Zn2+ | inhibitory at 10 mM, slightly activating at 1 mM | Leishmania amazonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62337 | - |
6 * 62337, recombinant His-tagged enzyme, amino acid sequence calculation | Leishmania donovani |
62424 | - |
6 * 62424, recombinant His-tagged enzyme, amino acid sequence calculation | Leishmania amazonensis |
62569 | - |
6 * 62569, recombinant His-tagged enzyme, amino acid sequence calculation | Leishmania major |
360000 | - |
gel filtration | Leishmania amazonensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania amazonensis | Q95V76 | - |
- |
Leishmania donovani | Q95V75 | - |
- |
Leishmania major | Q7KF27 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Leishmania donovani |
additional information | - |
- |
Leishmania major |
additional information | - |
- |
Leishmania amazonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-7-amido-4-methylcoumarin + H2O | low activity | Leishmania donovani | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | low activity | Leishmania major | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | low activity | Leishmania amazonensis | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Cys-7-amido-4-methylcoumarin + H2O | - |
Leishmania donovani | L-Cys + 7-amino-4-methylcoumarin | - |
? | |
L-Cys-7-amido-4-methylcoumarin + H2O | - |
Leishmania major | L-Cys + 7-amino-4-methylcoumarin | - |
? | |
L-Cys-7-amido-4-methylcoumarin + H2O | - |
Leishmania amazonensis | L-Cys + 7-amino-4-methylcoumarin | - |
? | |
L-Ile-7-amido-4-methylcoumarin + H2O | low activity | Leishmania donovani | L-Ile + 7-amino-4-methylcoumarin | - |
? | |
L-Ile-7-amido-4-methylcoumarin + H2O | low activity | Leishmania major | L-Ile + 7-amino-4-methylcoumarin | - |
? | |
L-Ile-7-amido-4-methylcoumarin + H2O | low activity | Leishmania amazonensis | L-Ile + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | best synthetic substrate | Leishmania donovani | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | best synthetic substrate | Leishmania major | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | best synthetic substrate | Leishmania amazonensis | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
L-Met-7-amido-4-methylcoumarin + H2O | - |
Leishmania donovani | L-Met + 7-amino-4-methylcoumarin | - |
? | |
L-Met-7-amido-4-methylcoumarin + H2O | - |
Leishmania major | L-Met + 7-amino-4-methylcoumarin | - |
? | |
L-Met-7-amido-4-methylcoumarin + H2O | - |
Leishmania amazonensis | L-Met + 7-amino-4-methylcoumarin | - |
? | |
L-Trp-7-amido-4-methylcoumarin + H2O | low activity | Leishmania donovani | L-Trp + 7-amino-4-methylcoumarin | - |
? | |
L-Trp-7-amido-4-methylcoumarin + H2O | low activity | Leishmania major | L-Trp + 7-amino-4-methylcoumarin | - |
? | |
L-Trp-7-amido-4-methylcoumarin + H2O | low activity | Leishmania amazonensis | L-Trp + 7-amino-4-methylcoumarin | - |
? | |
additional information | no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide | Leishmania donovani | ? | - |
? | |
additional information | no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide | Leishmania major | ? | - |
? | |
additional information | no activity with any other L-amino acid amide, N-carboxybenzyloxy-L-Leu-beta-naphthylamide, and N-carboxybenzyloxy-Gly-Gly-Leu-4-nitroanilide | Leishmania amazonensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 62337, recombinant His-tagged enzyme, amino acid sequence calculation | Leishmania donovani |
hexamer | 6 * 62424, recombinant His-tagged enzyme, amino acid sequence calculation | Leishmania amazonensis |
hexamer | 6 * 62569, recombinant His-tagged enzyme, amino acid sequence calculation | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
LAP | - |
Leishmania donovani |
LAP | - |
Leishmania major |
LAP | - |
Leishmania amazonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania donovani |
37 | - |
assay at | Leishmania major |
37 | - |
assay at | Leishmania amazonensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.057 | 0.65 | L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.07 | - |
L-Trp-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.09 | - |
L-Ile-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.19 | - |
L-Ile-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.19 | - |
L-Ile-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.21 | - |
L-Trp-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
0.22 | - |
L-Trp-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
0.41 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
0.52 | - |
L-Leu-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
1.61 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
1.89 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
2.01 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
2.19 | - |
L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
2.72 | - |
L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
2.94 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
2.94 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
2.94 | - |
L-Ala-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
2.94 | - |
L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major | |
3 | 6 | L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
3 | 6 | L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
3.3 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
3.3 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
3.34 | - |
L-Cys-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
4.99 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania amazonensis | |
5.08 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania donovani | |
5.52 | - |
L-Met-7-amido-4-methylcoumarin | pH 8.0, 37°C, recombinant His-tagged enzyme | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Leishmania donovani |
8 | - |
assay at | Leishmania major |
8.5 | - |
- |
Leishmania amazonensis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000008 | - |
actinonin | - |
Leishmania donovani | |
0.000001 | - |
actinonin | - |
Leishmania amazonensis | |
0.0000029 | - |
actinonin | - |
Leishmania major | |
0.000003 | - |
bestatin | - |
Leishmania amazonensis | |
0.000044 | - |
apstatin | - |
Leishmania amazonensis | |
0.059 | - |
arphamenine A | - |
Leishmania donovani | |
0.069 | - |
arphamenine A | - |
Leishmania major | |
0.07 | - |
arphamenine A | - |
Leishmania amazonensis | |
0.102 | - |
L-Leucinol | - |
Leishmania major | |
0.109 | - |
L-Leucinol | - |
Leishmania amazonensis | |
0.118 | - |
L-Leucinol | - |
Leishmania donovani |