Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 3.3% activity at 0.2 mM | Eisenia fetida | |
bestatin | 16.4% activity at 0.05 mM | Eisenia fetida | |
Ca2+ | partially inactivated at 1 mM | Eisenia fetida | |
Cd2+ | strongly inactivated at 1 mM | Eisenia fetida | |
Cu2+ | strongly inactivated at 1 mM | Eisenia fetida | |
EDTA | 40.7% activity at 5 mM, 6.7% activity after extensive dialysis against 5 mM, restored to 60.1% with 0.05 mM Zn2+ | Eisenia fetida | |
EGTA | 43.7% activity at 5 mM | Eisenia fetida | |
Fe2+ | strongly inactivated at 1 mM | Eisenia fetida | |
Leu | 41.5% activity at 1 mM | Eisenia fetida | |
Mn2+ | partially inactivated at 1 mM | Eisenia fetida | |
Phe | 33.5% activity at 1 mM | Eisenia fetida | |
Trp | 40.7% activity at 1 mM | Eisenia fetida | |
Zn2+ | partially inactivated at 1 mM | Eisenia fetida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0084 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
0.015 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
0.019 | - |
L-valine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
0.025 | - |
L-proline-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
0.054 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
0.055 | - |
L-tyrosine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
0.39 | - |
glycine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | slightly stimulated at 1 mM (111.6% activity) | Eisenia fetida | |
Mg2+ | slightly stimulated at 1 mM (122.7% activity) | Eisenia fetida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
1 * 98000, SDS-PAGE, gel filtration | Eisenia fetida |
100000 | - |
gel filtration | Eisenia fetida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eisenia fetida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydrophobic interaction chromatography, gel filtration, ion exchange chromatography (DEAE), hydroxyapatite chromatography, filtration through Heparin Sepharose | Eisenia fetida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.958 | - |
substrate L-leucine-4-methylcoumaryl-7-amide, pH 7.0, 37°C | Eisenia fetida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine-4-methylcoumaryl-7-amide + H2O | 3.9% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | glycine + 7-amino-4-methylcoumarin | - |
? | |
L-alanine-4-methylcoumaryl-7-amide + H2O | 32.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-alanine + 7-amino-4-methylcoumarin | - |
? | |
L-arginine-4-methylcoumaryl-7-amide + H2O | 62.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-arginine + 7-amino-4-methylcoumarin | - |
? | |
L-leucine-4-methylcoumaryl-7-amide + H2O | - |
Eisenia fetida | L-leucine + 7-amino-4-methylcoumarin | - |
? | |
L-proline-4-methylcoumaryl-7-amide + H2O | 1.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-proline + 7-amino-4-methylcoumarin | - |
? | |
L-tyrosine-4-methylcoumaryl-7-amide + H2O | 8.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-tyrosine + 7-amino-4-methylcoumarin | - |
? | |
L-valine-4-methylcoumaryl-7-amide + H2O | 2.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide | Eisenia fetida | L-valine + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 98000, SDS-PAGE, gel filtration | Eisenia fetida |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase | - |
Eisenia fetida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Eisenia fetida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 35 | 98% activity after 1.5h, sharp decrease above 45°C, less than 4% activity after 1.5h at 50°C | Eisenia fetida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-proline-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
1.5 | - |
L-valine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
12.1 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
13.8 | - |
L-tyrosine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
34.6 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
38.7 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
45.6 | - |
glycine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Eisenia fetida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Eisenia fetida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | lose activity rapidly below pH 6.0 or above pH 9.0 during incubation at 4°C for 24h | Eisenia fetida |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
L-proline-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
80 | - |
L-valine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
120 | - |
glycine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
250 | - |
L-tyrosine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
720 | - |
L-alanine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
1400 | - |
L-arginine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida | |
2300 | - |
L-leucine-4-methylcoumaryl-7-amide | pH 7.0, 37°C | Eisenia fetida |