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Literature summary for 3.4.11.1 extracted from

  • Chen, S.; Cao, M.; Su, W.; Wu, G.
    Purification and characterization of a novel leucine aminopeptidase from the earthworm Eisenia foetida (2011), Process Biochem., 46, 1641-1648.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 3.3% activity at 0.2 mM Eisenia fetida
bestatin 16.4% activity at 0.05 mM Eisenia fetida
Ca2+ partially inactivated at 1 mM Eisenia fetida
Cd2+ strongly inactivated at 1 mM Eisenia fetida
Cu2+ strongly inactivated at 1 mM Eisenia fetida
EDTA 40.7% activity at 5 mM, 6.7% activity after extensive dialysis against 5 mM, restored to 60.1% with 0.05 mM Zn2+ Eisenia fetida
EGTA 43.7% activity at 5 mM Eisenia fetida
Fe2+ strongly inactivated at 1 mM Eisenia fetida
Leu 41.5% activity at 1 mM Eisenia fetida
Mn2+ partially inactivated at 1 mM Eisenia fetida
Phe 33.5% activity at 1 mM Eisenia fetida
Trp 40.7% activity at 1 mM Eisenia fetida
Zn2+ partially inactivated at 1 mM Eisenia fetida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0084
-
L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
0.015
-
L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
0.019
-
L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
0.025
-
L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
0.054
-
L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
0.055
-
L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
0.39
-
glycine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ slightly stimulated at 1 mM (111.6% activity) Eisenia fetida
Mg2+ slightly stimulated at 1 mM (122.7% activity) Eisenia fetida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
1 * 98000, SDS-PAGE, gel filtration Eisenia fetida
100000
-
gel filtration Eisenia fetida

Organism

Organism UniProt Comment Textmining
Eisenia fetida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydrophobic interaction chromatography, gel filtration, ion exchange chromatography (DEAE), hydroxyapatite chromatography, filtration through Heparin Sepharose Eisenia fetida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.958
-
substrate L-leucine-4-methylcoumaryl-7-amide, pH 7.0, 37°C Eisenia fetida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine-4-methylcoumaryl-7-amide + H2O 3.9% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide Eisenia fetida glycine + 7-amino-4-methylcoumarin
-
?
L-alanine-4-methylcoumaryl-7-amide + H2O 32.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide Eisenia fetida L-alanine + 7-amino-4-methylcoumarin
-
?
L-arginine-4-methylcoumaryl-7-amide + H2O 62.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide Eisenia fetida L-arginine + 7-amino-4-methylcoumarin
-
?
L-leucine-4-methylcoumaryl-7-amide + H2O
-
Eisenia fetida L-leucine + 7-amino-4-methylcoumarin
-
?
L-proline-4-methylcoumaryl-7-amide + H2O 1.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide Eisenia fetida L-proline + 7-amino-4-methylcoumarin
-
?
L-tyrosine-4-methylcoumaryl-7-amide + H2O 8.5% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide Eisenia fetida L-tyrosine + 7-amino-4-methylcoumarin
-
?
L-valine-4-methylcoumaryl-7-amide + H2O 2.3% activity in comparison to L-leucine-4-methyl-coumaryl-7-amide Eisenia fetida L-valine + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 98000, SDS-PAGE, gel filtration Eisenia fetida

Synonyms

Synonyms Comment Organism
leucine aminopeptidase
-
Eisenia fetida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Eisenia fetida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 35 98% activity after 1.5h, sharp decrease above 45°C, less than 4% activity after 1.5h at 50°C Eisenia fetida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
1.5
-
L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
12.1
-
L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
13.8
-
L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
34.6
-
L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
38.7
-
L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
45.6
-
glycine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Eisenia fetida

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9
-
Eisenia fetida

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 lose activity rapidly below pH 6.0 or above pH 9.0 during incubation at 4°C for 24h Eisenia fetida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
50
-
L-proline-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
80
-
L-valine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
120
-
glycine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
250
-
L-tyrosine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
720
-
L-alanine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
1400
-
L-arginine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida
2300
-
L-leucine-4-methylcoumaryl-7-amide pH 7.0, 37°C Eisenia fetida