Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3) | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.25 mM: 17% activity, restored by Mn2+ and Ca2+ | Trypanosoma cruzi | |
bestatin | 0.1 mM: 3% activity, activity | Trypanosoma cruzi | |
EDTA | 10 mM: 55% activity, restored by Mn2+ and Ca2+ | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
L-Leu-7-amido-4-methylcoumarin | native enzyme, pH 7.5, 37°C | Trypanosoma cruzi | |
0.1859 | - |
L-Leu-7-amido-4-methylcoumarin | recombinant enzyme, pH 7.5, 37°C | Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
6 * 55000, SDS-PAGE, calculated: 55891 Da | Trypanosoma cruzi |
320000 | - |
gel filtration | Trypanosoma cruzi |
330000 | - |
analytical ultracentrifugation | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q4DZJ3 | - |
- |
Purification (Comment) | Organism |
---|---|
ion exchange chromatography (DEAE), gel filtration; His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), gel filtration | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma cruzi | L-Leu + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 55000, SDS-PAGE, calculated: 55891 Da | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
major leucyl aminopeptidase | metalloaminopeptidase | Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
native enzyme | Trypanosoma cruzi |
60 | - |
recombinant enzyme, 25% activity at 37°C | Trypanosoma cruzi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
recombinant enzyme is more stable than native enzyme: after 15 min at 60°C native form has 35% and recombinant form 87% activity | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.47 | - |
L-Leu-7-amido-4-methylcoumarin | native enzyme, pH 7.5, 37°C | Trypanosoma cruzi | |
34.84 | - |
L-Leu-7-amido-4-methylcoumarin | recombinant enzyme, pH 7.5, 37°C | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
native enzyme | Trypanosoma cruzi |
8 | - |
recombinant enzyme | Trypanosoma cruzi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | native enzyme, 45% activity at pH 6.0 and 8.0, inactive at pH 5.0 and 9.0 | Trypanosoma cruzi |
7.5 | 9 | recombinant enzyme, 40% activity at pH 7.5, 75% activity at pH 9.0 | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
190 | - |
L-Leu-7-amido-4-methylcoumarin | recombinant enzyme, pH 7.5, 37°C | Trypanosoma cruzi | |
1040 | - |
L-Leu-7-amido-4-methylcoumarin | native enzyme, pH 7.5, 37°C | Trypanosoma cruzi |