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Literature summary for 3.4.11.1 extracted from

  • Carroll, R.K.; Veillard, F.; Gagne, D.T.; Lindenmuth, J.M.; Poreba, M.; Drag, M.; Potempa, J.; Shaw, L.N.
    The Staphylococcus aureus leucine aminopeptidase is localized to the bacterial cytosol and demonstrates a broad substrate range that extends beyond leucine (2013), Biol. Chem., 394, 791-803.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.79
-
L-Arg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
0.82
-
hArg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
1.33
-
Phg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
1.38
-
L-Leu-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Staphylococcus aureus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enzyme not functional in the presence of Staphylococcus aureus
Cd2+ functional in the presence of, highest activity at pH 9 Staphylococcus aureus
Co2+ enzyme not functional in the presence of Staphylococcus aureus
Mg2+ enzyme not functional in the presence of Staphylococcus aureus
Mn2+ 0.2 mM, increases activity by 7fold Staphylococcus aureus
Ni2+ functional in the presence of, highest activity at pH 7 Staphylococcus aureus
Zn2+ enzyme not functional in the presence of Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hArg-7-amido-4-methylcoumarin + H2O
-
Staphylococcus aureus hArg + 7-amino-4-methylcoumarin
-
?
L-Ala-7-amido-4-methylcoumarin + H2O
-
Staphylococcus aureus L-Ala + 7-amino-4-methylcoumarin
-
?
L-Arg-7-amido-4-methylcoumarin + H2O
-
Staphylococcus aureus L-Arg + 7-amino-4-methylcoumarin
-
?
L-Leu-7-amido-4-methylcoumarin + H2O
-
Staphylococcus aureus L-Leu + 7-amino-4-methylcoumarin
-
?
additional information the substrate range of LAP is quite complex. L-Leu is not the preferred substrate of the enzyme Staphylococcus aureus ?
-
?
Phg-7-amido-4-methylcoumarin + H2O
-
Staphylococcus aureus Phg + 7-amino-4-methylcoumarin
-
?

Synonyms

Synonyms Comment Organism
LAP enzyme also possesses cysteinylglycinase activity Staphylococcus aureus
leucine aminopeptidase
-
Staphylococcus aureus
pepZ
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1204
-
L-Leu-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
0.1395
-
L-Arg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
0.1584
-
hArg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
0.3952
-
Phg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
highest level of activity observed in TAPS or CAPS buffer. All enzymatic analyses done in TAPS buffer Staphylococcus aureus

General Information

General Information Comment Organism
malfunction inhibition of LAP with bestatin does not adversely affect growth of Staphylococcus aureus or biofilm formation Staphylococcus aureus
malfunction lysates from pepZ mutant cells show leucine aminopeptidase activity comparable to that of the wild-type strain suggesting that the majority of leucine aminopeptidase activity observed in Staphylococcus aureus cells comes from PepS, and not LAP Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.08725
-
L-Leu-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
0.176
-
L-Arg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
0.193
-
hArg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus
0.297
-
Phg-7-amido-4-methylcoumarin pH 9, 37°C Staphylococcus aureus