Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.79 | - |
L-Arg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
0.82 | - |
hArg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
1.33 | - |
Phg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
1.38 | - |
L-Leu-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Staphylococcus aureus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | enzyme not functional in the presence of | Staphylococcus aureus | |
Cd2+ | functional in the presence of, highest activity at pH 9 | Staphylococcus aureus | |
Co2+ | enzyme not functional in the presence of | Staphylococcus aureus | |
Mg2+ | enzyme not functional in the presence of | Staphylococcus aureus | |
Mn2+ | 0.2 mM, increases activity by 7fold | Staphylococcus aureus | |
Ni2+ | functional in the presence of, highest activity at pH 7 | Staphylococcus aureus | |
Zn2+ | enzyme not functional in the presence of | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hArg-7-amido-4-methylcoumarin + H2O | - |
Staphylococcus aureus | hArg + 7-amino-4-methylcoumarin | - |
? | |
L-Ala-7-amido-4-methylcoumarin + H2O | - |
Staphylococcus aureus | L-Ala + 7-amino-4-methylcoumarin | - |
? | |
L-Arg-7-amido-4-methylcoumarin + H2O | - |
Staphylococcus aureus | L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Leu-7-amido-4-methylcoumarin + H2O | - |
Staphylococcus aureus | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
additional information | the substrate range of LAP is quite complex. L-Leu is not the preferred substrate of the enzyme | Staphylococcus aureus | ? | - |
? | |
Phg-7-amido-4-methylcoumarin + H2O | - |
Staphylococcus aureus | Phg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LAP | enzyme also possesses cysteinylglycinase activity | Staphylococcus aureus |
leucine aminopeptidase | - |
Staphylococcus aureus |
pepZ | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1204 | - |
L-Leu-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
0.1395 | - |
L-Arg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
0.1584 | - |
hArg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
0.3952 | - |
Phg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
highest level of activity observed in TAPS or CAPS buffer. All enzymatic analyses done in TAPS buffer | Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
malfunction | inhibition of LAP with bestatin does not adversely affect growth of Staphylococcus aureus or biofilm formation | Staphylococcus aureus |
malfunction | lysates from pepZ mutant cells show leucine aminopeptidase activity comparable to that of the wild-type strain suggesting that the majority of leucine aminopeptidase activity observed in Staphylococcus aureus cells comes from PepS, and not LAP | Staphylococcus aureus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08725 | - |
L-Leu-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
0.176 | - |
L-Arg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
0.193 | - |
hArg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus | |
0.297 | - |
Phg-7-amido-4-methylcoumarin | pH 9, 37°C | Staphylococcus aureus |