Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
amastatin | - |
Homo sapiens | |
bestatin | - |
Homo sapiens | |
EDTA | reactivation by divalent cations | Homo sapiens | |
iodoacetic acid | - |
Homo sapiens | |
N-ethylmaleimide | - |
Homo sapiens | |
p-chloromercuribenzoate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.02 | - |
leucinamide | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Homo sapiens | |
Mn2+ | activation | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
326000 | 360000 | non-denaturing PAGE, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58 | - |
substrate L-Leu-amide | Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, 100 mM Tris-HCl buffer, pH 8.0, 6 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amino acid amides + H2O | - |
Homo sapiens | amino acid + NH3 | - |
? | |
L-Leu-p-nitroanilide + H2O | no hydrolysis | Homo sapiens | L-leucine + p-nitroaniline | - |
? | |
L-leucine amide + H2O | - |
Homo sapiens | L-leucine + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 3 * ab, a: 53000, b: 65000, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
2 weeks | Homo sapiens |
37 | - |
1 day | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10.4 | 10.7 | - |
Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Homo sapiens |