Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.1 extracted from

  • Scranton, M.A.; Yee, A.; Park, S.Y.; Walling, L.L.
    Plant leucine aminopeptidases moonlight as molecular chaperones to alleviate stress-induced damage (2012), J. Biol. Chem., 287, 18408-18417.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
D347N mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum
D347R inactive mutant with disrupted hexameric structure has increased chaperone activity Solanum lycopersicum
E429R inactive mutant with disrupted hexameric structure has increased chaperone activity Solanum lycopersicum
E429V mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum
K354E inactive mutant with disrupted hexameric structure has increased chaperone activity Solanum lycopersicum
K354R mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum
additional information LAP-A exhibits chaperone activity which is independant of its peptidase activity Solanum lycopersicum
R431A mutation leads to inactivation of peptidase activity but does not have an impact on chaperone activity Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Solanum lycopersicum 9507
-

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q10712
-
-
Solanum lycopersicum Q8GZD8
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu-4-nitroanilide + H2O
-
 Solanum lycopersicum L-Leu + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
hexamer disruption of hexameric structure increases in vitro chaperone activity Solanum lycopersicum

Synonyms

Synonyms Comment Organism
LAP-A stress-induced acidic LAPs, LAP-A exhibits chaperone activity Solanum lycopersicum
LAP-N neutral LAP, LAP-N exhibtis chaperone activity Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Solanum lycopersicum

General Information

General Information Comment Organism
malfunction disruption of the LAP-A hexameric structure increases chaperone activity Solanum lycopersicum
physiological function LAP-A exhibits chaperone activity which is independant of its peptidase activity Solanum lycopersicum