Cloned (Comment) | Organism |
---|---|
gene LAP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Echinococcus granulosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | no transmembrane regions or signal peptide sequences are predicted in the deduced amino acid sequence | Echinococcus granulosus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Echinococcus granulosus | |
Mn2+ | activates | Echinococcus granulosus | |
Ni2+ | activates | Echinococcus granulosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Echinococcus granulosus | A0A336UEJ6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Echinococcus granulosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | localization of the enzyme protein in the parasite in sheep using fluorescence immunohistochemistry, development of an ELISA method, efficient and effective for early stage diagnosis of infection and the detection of infected animals, overview | Echinococcus granulosus | - |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 65800, about, sequence calculation, 6 * 66000, native enzyme, SDS-PAGE, 6 * 72000, recombinant His-tagged enzyme, SDS-PAGE | Echinococcus granulosus |
Synonyms | Comment | Organism |
---|---|---|
Eg-LAP | - |
Echinococcus granulosus |
LAP | - |
Echinococcus granulosus |
leucine aminopeptidase | - |
Echinococcus granulosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Echinococcus granulosus | sequence calculation | - |
6.6 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the M17 peptidase family | Echinococcus granulosus |
physiological function | leucine aminopeptidases (LAPs) of the M17 peptidase family have important functions in regulating the balance of catabolism and anabolism, cell maintenance, growth and defense | Echinococcus granulosus |