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Literature summary for 3.4.11.1 extracted from

  • Karadzic, I.; Izrael, L.; Gojgic-Cvijovic, G.; Vujcic, Z.
    Leucine aminopeptidase from Streptomyces hygroscopicus is controlled by a low molecular weight inhibitor (2002), J. Biosci. Bioeng., 94, 309-314.
    View publication on PubMed

Application

Application Comment Organism
medicine production of polyketide antibiotics Streptomyces hygroscopicus
synthesis production of polyketide antibiotics Streptomyces hygroscopicus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline strong inhibition Streptomyces hygroscopicus
amastatin strong inhibition Streptomyces hygroscopicus
autogenous low molecular weight inhibitor regulatory function, modulation during periods of intensive polyketide antibiotics formation Streptomyces hygroscopicus
DTT strong inhibition Streptomyces hygroscopicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 2 mol of Zn2+ per mol of enzyme Streptomyces hygroscopicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
gel filtration Streptomyces hygroscopicus
67000
-
1 * 67000, SDS-PAGE Streptomyces hygroscopicus

Organism

Organism UniProt Comment Textmining
Streptomyces hygroscopicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
4573fold Streptomyces hygroscopicus

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low prefers leucine and methionine as N-terminal amino acids Streptomyces hygroscopicus

Source Tissue

Source Tissue Comment Organism Textmining

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE Streptomyces hygroscopicus

Synonyms

Synonyms Comment Organism
leucine aminopeptidase
-
Streptomyces hygroscopicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces hygroscopicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
stable up to Streptomyces hygroscopicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptomyces hygroscopicus

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces hygroscopicus
-
-
8.2