Literature summary for 3.4.11.1 extracted from

Lin, S.; Chen, L.; Wen, C.; Chu, W.
Extracellular leucine aminopeptidase produced by Aspergillus oryzae LL1 and LL2 (2010), Afr. J. Microbiol. Res., 4, 158-168.

No PubMed abstract available

Inhibitors

Inhibitors	Comment	Organism
Cu2+	2 mM: 4.5% activity in crude enzyme solution	Aspergillus oryzae
Fe2+	2 mM: 37.9% activity in crude enzyme solution	Aspergillus oryzae
Pb2+	2 mM: 5.9% activity in crude enzyme solution	Aspergillus oryzae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	2 mM: 184.3% activity in crude enzyme solution	Aspergillus oryzae

Organism

Organism	UniProt	Comment	Textmining
Aspergillus oryzae	-	-	-
Aspergillus oryzae LL1	-	-	-

Storage Stability

Storage Stability	Organism
Storage of crude enzyme solutions at -80, -20, 4 and 25°C for eight weeks reduces activity after the first three weeks of storage at all temperatures. At three weeks, residual activities are inversely proportional to their associated storage temperatures, with activity being reduced to 84, 77, 75 and 64% at temperatures of -80, -20, 4 and 25°C, respectively. Activity remained relatively stable from 4-8 weeks at temperatures of -80, -20 and 25°C. In contrast, samples stored at 4°C exhibit an additional about 10% decrease in activity during this time.	Aspergillus oryzae

Organism

Storage of crude enzyme solutions at -80, -20, 4 and 25°C for eight weeks reduces activity after the first three weeks of storage at all temperatures. At three weeks, residual activities are inversely proportional to their associated storage temperatures, with activity being reduced to 84, 77, 75 and 64% at temperatures of -80, -20, 4 and 25°C, respectively. Activity remained relatively stable from 4-8 weeks at temperatures of -80, -20 and 25°C. In contrast, samples stored at 4°C exhibit an additional about 10% decrease in activity during this time.

Aspergillus oryzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-L-Leu-4-nitroanilide + H2O	-	Aspergillus oryzae	N-succinyl-L-Leu + 4-nitroaniline	-	?
N-succinyl-L-Leu-4-nitroanilide + H2O	-	Aspergillus oryzae LL1	N-succinyl-L-Leu + 4-nitroaniline	-	?

Synonyms

Synonyms	Comment	Organism
leucine aminopeptidase	extracellular	Aspergillus oryzae

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Release 2023.1 (January 2023)