Any feedback?
Literature summary for 3.4.11.1 extracted from
- Small-molecule probe using dual signals to monitor leucine aminopeptidase activity (2011), Bioorg. Med. Chem. Lett., 21, 2403-2405.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bestatin | slow-binding, competitive | Sus scrofa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00887 | - |
4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate | pH 7.4, 25°C | Sus scrofa | |
| 0.065 | - |
N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide | pH not specified in the publication, temperature not specified in the publication | Sus scrofa | |
| 3 | - |
L-leucine-4-nitroanilide | pH not specified in the publication, temperature not specified in the publication | Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Sus scrofa | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| commercial preparation | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.9 | - |
L-leucine-4-nitroanilide, pH not specified in the publication, temperature not specified in the publication | Sus scrofa |
| 6.64 | - |
4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate, pH 7.4, 25°C | Sus scrofa |
| 7.7 | - |
N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide, pH not specified in the publication, temperature not specified in the publication | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate + H2O | - |
Sus scrofa | L-leucine + 4-[(E)-2-(4-aminophenyl)ethenyl-3-cyano-5,5-dimethylfuran-2(5H)-ylidene]propanedinitrile | - |
? | |
| L-leucine-4-nitroanilide + H2O | - |
Sus scrofa | L-leucine + 4-nitroaniline | - |
? | |
| N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide + H2O | - |
Sus scrofa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| leucine aminopeptidase | - |
Sus scrofa |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00441 | - |
substrate 4-[(E)-2-[4-cyano-5-(dicyanomethylidene)-2,2-dimethyl-2,5-dihydrofuran-3-yl]ethenyl]phenyl L-leucinate, pH 7.4, 25°C | Sus scrofa | bestatin | |
| 0.007 | - |
substrate N-(6-methoxy-2-methylpyridin-3-yl),(S)-2-amino-4-methylpentanamide, pH not specified in the publication, temperature not specified in the publication | Sus scrofa | bestatin | |
| 0.025 | - |
substrate L-leucine-4-nitroanilide, pH not specified in the publication, temperature not specified in the publication | Sus scrofa | bestatin | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
