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Literature summary for 3.4.11.1 extracted from

  • Hatta, T.; Kazama, K.; Miyoshi, T.; Umemiya, R.; Liao, M.; Inoue, N.; Xuan, X.; Tsuji, N.; Fujisaki, K.
    Identification and characterisation of a leucine aminopeptidase from the hard tick Haemaphysalis longicornis (2006), Int. J. Parasitol., 36, 1123-1132.
    View publication on PubMed
Search for more literature for 3.4.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Haemaphysalis longicornis

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
 Haemaphysalis longicornis
Cu2+ 10 mM Haemaphysalis longicornis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 L-methionine-4-methyl-coumaryl-7-amide
-
 Haemaphysalis longicornis
0.19
-
 L-leucine-4-methylcoumaryl-7-amide
-
 Haemaphysalis longicornis
0.19
-
 L-arginine-4-methylcoumaryl-7-amide
-
 Haemaphysalis longicornis
0.3
-
 L-phenylalanine-4-methyl-coumaryl-7-amide
-
 Haemaphysalis longicornis
0.81
-
 L-lysine-4-methylcoumaryl-7-amide
-
 Haemaphysalis longicornis
1.16
-
 L-alanine-4-methylcoumaryl-7-amide
-
 Haemaphysalis longicornis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Haemaphysalis longicornis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 597fold activation at 1 mM compared with non-metal ion-supplemented condition Haemaphysalis longicornis
Cu2+ 4fold activation at 0.1 mM compared with non-metal ion-supplemented condition Haemaphysalis longicornis
Fe2+ 12fold activation at 1 mM compared with non-metal ion-supplemented condition Haemaphysalis longicornis
Mg2+ 120fold activation at 10 mM compared with non-metal ion-supplemented condition Haemaphysalis longicornis
Mn2+ maximal activation at 5 mM. 1849fold activation at 10 mM compared with non-metal ion-supplemented condition Haemaphysalis longicornis
Ni2+ 493fold activation at 10 mM compared with non-metal ion-supplemented condition Haemaphysalis longicornis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56420
-
 x * 56420, calculated from sequence Haemaphysalis longicornis

Organism

Organism UniProt Comment Textmining
Haemaphysalis longicornis Q0KKA6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haemaphysalis longicornis

Source Tissue

Source Tissue Comment Organism Textmining
epithelial cell from midgut Haemaphysalis longicornis
-
midgut epithelial cells Haemaphysalis longicornis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-methylcoumaryl-7-amide + H2O
-
 Haemaphysalis longicornis L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-arginine-4-methylcoumaryl-7-amide + H2O
-
 Haemaphysalis longicornis L-arginine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-4-methylcoumaryl-7-amide + H2O
-
 Haemaphysalis longicornis L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-lysine-4-methylcoumaryl-7-amide + H2O
-
 Haemaphysalis longicornis L-lysine + 7-amino-4-methylcoumarin
-
 ?
L-methionine-4-methylcoumaryl-7-amide + H2O
-
 Haemaphysalis longicornis L-methionine + 7-amino-4-methylcoumarin
-
 ?
L-phenylalanine-4-methylcoumaryl-7-amide + H2O
-
 Haemaphysalis longicornis L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 56420, calculated from sequence Haemaphysalis longicornis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Haemaphysalis longicornis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Haemaphysalis longicornis

pI Value

Organism Comment pI Value Maximum pI Value
Haemaphysalis longicornis calculated from sequence
-
 8.7