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Literature summary for 3.4.11.1 extracted from

  • Laurinyecz, B.; Vedelek, V.; Kovacs, A.L.; Szilasi, K.; Lipinszki, Z.; Slezak, C.; Darula, Z.; Juhasz, G.; Sinka, R.
    Sperm-leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm (2019), PLoS Genet., 15, e1007987 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant Mi(ET)S-Lap3MB01319, overview Drosophila melanogaster
additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant Mi(ET1)S-Lap4MB11296, overview Drosophila melanogaster
additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant Mi(MIC)S-Lap2MI14597, overview Drosophila melanogaster
additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, mutant S-Lap8KK106866, overview Drosophila melanogaster
additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, overview Drosophila melanogaster
additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, overview. Despite the mitochondrial localization, S-Lap1resc-HA construct is not able to rescue the male sterile phenotype of the S-Lap1DELTA7 mutant, indicating that the C-terminal tag may modify the structure of the protein, which could prevent its normal function Drosophila melanogaster
additional information construction of mutants of lap genes S-Lap1 to S-Lap8, comparisons of the phenotypes of the mutant alleles of S-Lap genes, overview. Despite the mitochondrial localization, S-Lap1resc-HA construct is not able to rescue the male sterile phenotype of the S-Lap1DELTA7 mutant, indicating that the C-terminal tag may modify the structure of the protein, which could prevent its normal function. Strong reduction of S-Lap1-HAint in the pellet fraction of testis samples from the S-Lap1-HAint-containing S-Lap2MI14597, S-Lap3MB01319, S-Lap4MB11296, S-Lap5DELTA14, and S-Lap6MI06848 mutants, where the S-Lap1-HAint protein is present mainly in the supernatant, suggesting a problem with paracrystalline formation Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion two mitochondrial derivatives differentiate and by the end of spermatogenesis the minor one reduces its size and the major one accumulates paracrystalline material inside it. The S-Lap enzymes are important constituents of the paracrystalline material in mitochondria of Drosophila melanogaster sperm. S-Lap proteins localize and accumulate in the paracrystalline material of the major mitochondrial derivative, S-Lap proteins are collectively present in the isolates Drosophila melanogaster 5739
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Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Drosophila melanogaster
Zn2+ required Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q500X4
-
-
Drosophila melanogaster Q7K2S9
-
-
Drosophila melanogaster Q7K5K9
-
-
Drosophila melanogaster Q8T4F1
-
-
Drosophila melanogaster Q95R35
-
-
Drosophila melanogaster Q961W5
-
-
Drosophila melanogaster Q9VSM6
-
-
Drosophila melanogaster Q9VSM7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
semen
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Drosophila melanogaster
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testis
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Drosophila melanogaster
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Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Drosophila melanogaster

Synonyms

Synonyms Comment Organism
leucylaminopeptidase
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Drosophila melanogaster
S-Lap1
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Drosophila melanogaster
S-Lap2
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Drosophila melanogaster
S-Lap3
-
Drosophila melanogaster
S-Lap4
-
Drosophila melanogaster
S-Lap5
-
Drosophila melanogaster
S-Lap6
-
Drosophila melanogaster
S-Lap7
-
Drosophila melanogaster
S-Lap8
-
Drosophila melanogaster
sperm-leucylaminopeptidase
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Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Drosophila melanogaster

General Information

General Information Comment Organism
malfunction S-Lap1 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
malfunction S-Lap2 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
malfunction S-Lap3 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
malfunction S-Lap4 gene mutant homozygotes are male semi-sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
malfunction S-Lap5 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
malfunction S-Lap6 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
malfunction S-Lap7 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
malfunction S-Lap8 gene mutant homozygotes are male sterile. The male sterile phenotype of S-Lap enzyme mutants is caused by defects in paracrystalline material accumulation and abnormal structure of the elongated major mitochondrial derivatives and elongated spermatids Drosophila melanogaster
physiological function sperm-leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm Drosophila melanogaster