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Literature summary for 3.4.11.1 extracted from

  • Huang, W.Q.; Zhong, L.F.; Meng, Z.Z.; You, Z.J.; Li, J.Z.; Luo, X.C.
    The structure and enzyme characteristics of a recombinant leucine aminopeptidase rLap1 from Aspergillus sojae and its application in debittering (2015), Appl. Biochem. Biotechnol., 177, 190-206 .
    View publication on PubMed
Search for more literature for 3.4.11.1

Application

Application Comment Organism
food industry application of recombinant leucine aminopeptidase rLap1 from Aspergillus sojae in debittering Aspergillus sojae

Cloned(Commentary)

Cloned (Comment) Organism
gene lap1, recombinant overexpression of truncated Lap1 without a signal peptide in Pichia pastoris Aspergillus sojae

Inhibitors

Inhibitors Comment Organism Structure
EDTA significant inhibition, Co2+, Mn2+, and Ca2+ ions, but not Zn2+ ions, restore the enzyme activity Aspergillus sojae

Organism

Organism UniProt Comment Textmining
Aspergillus sojae Q8J2N2
-
-
Aspergillus sojae GIM3.30 Q8J2N2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg 4-nitroanilide + H2O
-
 Aspergillus sojae Arg + 4-nitroaniline
-
 ?
Arg 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Arg + 4-nitroaniline
-
 ?
Leu 4-nitroanilide + H2O
-
 Aspergillus sojae Leu + 4-nitroaniline
-
 ?
Leu 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Leu + 4-nitroaniline
-
 ?
Lys 4-nitroanilide + H2O
-
 Aspergillus sojae Lys + 4-nitroaniline
-
 ?
Lys 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Lys + 4-nitroaniline
-
 ?
Met 4-nitroanilide + H2O
-
 Aspergillus sojae Met + 4-nitroaniline
-
 ?
Met 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Met + 4-nitroaniline
-
 ?
additional information rLap1 shows the highest activity against Arg-pNA, followed by Leu-, Lys-, Met-, and Phe-4-nitroanilide in descending order Aspergillus sojae ?
-
 ?
additional information rLap1 shows the highest activity against Arg-pNA, followed by Leu-, Lys-, Met-, and Phe-4-nitroanilide in descending order Aspergillus sojae GIM3.30 ?
-
 ?
Phe 4-nitroanilide + H2O
-
 Aspergillus sojae Phe + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 36700, recombinant enzyme lacking the signal peptide, SDS-PAGE Aspergillus sojae

Synonyms

Synonyms Comment Organism
LAP1
-
 Aspergillus sojae
leucine aminopeptidase
-
 Aspergillus sojae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 recombinant enzyme Aspergillus sojae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinant enzyme, 1 h, 50% activity remaining Aspergillus sojae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Aspergillus sojae

General Information

General Information Comment Organism
additional information recombinant Lap1 enzyme has a conserved functional charge/dipole complex and a hydrogen bond network of Zn2-D179-S228-Q177-D229-S158 around its active center. An acidic Asp residue is found at the bottom of the substrate binding pocket, which explains its preference for basic N-terminal amino acid substrates such as Arg and Lys. Three-dimensional structure modelling of rLap1 and substrate-docking analysis Aspergillus sojae
physiological function enzyme Lap1 improves the degree of hydrolysis of casein and soy protein hydrolysates and also decreases their bitterness, indicating its potential utility in food production Aspergillus sojae