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Literature summary for 3.4.11.1 extracted from

  • Prescott, J.M.; Wilkes, S.H.
    Aeromonas aminopeptidase (1976), Methods Enzymol., 45, 530-543.
    View publication on PubMed
Search for more literature for 3.4.11.1

General Stability

General Stability Organism
no inactivation Vibrio proteolyticus
urea 8 M, partial inactivation Vibrio proteolyticus

Inhibitors

Inhibitors Comment Organism Structure
(CH3)2CHCH2CH(NH2)CO-X X: e.g. succinamide,-CH3,-CH2Cl Vibrio proteolyticus
1,10-phenanthroline
-
 Vibrio proteolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 L-Leu-2-naphthylamide similar value Vibrio proteolyticus
0.51
-
 L-Leu-L-Val similar value Vibrio proteolyticus
4.02
-
 leucinamide similar value Vibrio proteolyticus
7.2
-
 L-Leu-Gly similar value Vibrio proteolyticus
8.5
-
 L-Leu methyl ester
-
 Vibrio proteolyticus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Vibrio proteolyticus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 4-6 gatom per mol Vibrio proteolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
 calculation from sedimentation and diffusion constants Vibrio proteolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Vibrio proteolyticus
-
 ?
-
 ?
Proteins + H2O Vibrio proteolyticus glucagon ?
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio proteolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio proteolyticus

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
 Vibrio proteolyticus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay methods Vibrio proteolyticus

Storage Stability

Storage Stability Organism
-20°C, apoenzyme or active enzyme, several years Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-amino acid-peptide + H2O leucyl-dipeptides Vibrio proteolyticus amino acid + peptide
-
 ?
L-amino acid-peptide + H2O Leu-Met Vibrio proteolyticus amino acid + peptide
-
 ?
L-amino acid-peptide + H2O Pro-Phe-Lys Vibrio proteolyticus amino acid + peptide
-
 ?
L-Leu methyl ester
-
 Vibrio proteolyticus ?
-
 ?
L-Leu-2-naphthylamide + H2O
-
 Vibrio proteolyticus L-Leu + 2-naphthylamine
-
 ?
L-Leu-Gly + H2O
-
 Vibrio proteolyticus L-Leu + Gly
-
 ?
L-leucine amide + H2O
-
 Vibrio proteolyticus L-leucine + NH3
-
 ?
Leu-Val + H2O
-
 Vibrio proteolyticus Leu + Val
-
 ?
additional information
-
 Vibrio proteolyticus ?
-
 ?
additional information esterase activity Vibrio proteolyticus ?
-
 ?
Proteins + H2O glucagon Vibrio proteolyticus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 several h Vibrio proteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Vibrio proteolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 8.5
-
 Vibrio proteolyticus