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3.5.1.1: asparaginase

This is an abbreviated version!
For detailed information about asparaginase, go to the full flat file.

Word Map on EC 3.5.1.1

Reaction

L-asparagine
+
H2O
=
L-aspartate
 +
NH3

Synonyms

14270 ASNase, alpha-asparaginase, AnsA, ansA3, AnsB, ansZ, Asn, ASNase, ASNase1, ASNase3, ASP1, ASP3, asparaginase, asparaginase II, asparagine amidohydrolase, asparginase II, ASPG, ASPG II, ASPGA1, ASPGB1, ASRGL1, At3g16150, colaspase, crasnitin, DiAsp, EcAII, EcAIII, EcaL-ASNase, ECAR-LANS, elspar, ErA, Erwinase, glutaminase-free L-asparaginase, glutamine-(asparagin-)ase, HPA, L-ASNase, L-asparaginase, L-asparaginase 2, L-asparaginase I, L-asparaginase II, L-asparaginase III, L-asparaginase type II, L-asparaginase type III, L-asparaginase-I, L-asparaginase-II, L-asparagine amidohydrolase, L-asparagine amino hydrolase, L-asparagine aminohydrolase, leunase, PfA, PhA, potassium-dependent asparaginase, potassium-independent asparaginase, potassium-independent L-asparaginase, SAMN05216263_10744, SGR ASNase, type I L-asparaginase, type II L-asparaginase, Ypa

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.1 asparaginase

Molecular Weight

Molecular Weight on EC 3.5.1.1 - asparaginase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000 - 120000
-
gel filtration
1000000
-
L-asparaginase I, gel filtration
102000
recombinant His-tagged enzyme, native PAGE
105000
-
gel filtration
108000
Erwinia aroidea
-
gel filtration
118000
-
gel filtration
12000
1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE
120000
122000
-
ultracentrifugation
126000
-
gel filtration
128000 - 145000
-
-
130000
132000
-
gel filtration
133000
-
gel filtration
133500
gel filtration
133700
gel filtration
134800
-
gel filtration
135000
138000
-
ultracentrifugation
138000 - 141000
-
-
140000
14200
x * 27000 + x * 14200, SDS-PAGE
144000
-
PAGE
144400
-
native PAGE, gel filtration, and MALDI-TOF mass spectrophotometry analysis
147000
15000
-
1 * 20000 + 1 * 15000, active, cleaved enzyme
150000
160000
160000 - 171000
-
sucrose density gradient centrifugation
165000
-
sucrose density gradient centrifugation
178000
gel filtration
185000
20000
200000
216000
-
gel filtration
25000
-
4 * 25000, SDS-PAGE
27000
x * 27000 + x * 14200, SDS-PAGE
275000
Chlamydomonas sp.
-
-
306000
-
gel filtration
33000
33700
-
4 * 33700, SDS-PAGE
34000
34500
35000
35690
-
calculated
36000
36100
-
4 * 36100, MALDI-TOF mass spectrophotometry analysis
36500
37500
2 * 37500, SDS-PAGE
37860
-
2 * 37000, SDS-PAGE, 2 * 37860, mass spectrometry
38000
-
x * 38000, SDS-PAGE
39000
-
x * 34500, about, sequence calculation, x * 39000, SDS-PAGE
400000
-
enzyme I
45000
-
x * 45000, SDS-PAGE, recombinant protein
47000
50000
-
x * 50000, L-asparaginase I, SDS-PAGE
543000
-
4 * 543000, SDS-PAGE
61000
-
potassium dependent enzyme, gel filtration
68300
-
-
70000
-
gel filtration
71000
72000
75000
75316
2 * 75316, calculated from amino acid sequence
75450
-
gel filtration, Superdex-200 column, single peak
79000
-
gel filtration
800000
-
enzyme II
83900
-
sucrose density gradient centrifugation
85000
93000
-
x * 93000, L-asparaginase II, SDS-PAGE
96000
Stenotrophomonas geniculata
-
enzyme A
97000
-
gel filtration
97400
-
SDS-PAGE
additional information