3.5.1.1: asparaginase

This is an abbreviated version!
For detailed information about asparaginase, go to the full flat file.

Reaction

Synonyms

14270 ASNase, alpha-asparaginase, AnsA, ansA3, AnsB, ansZ, Asn, ASNase, ASNase1, ASNase3, ASP1, ASP3, asparaginase, asparaginase II, asparagine amidohydrolase, asparginase II, ASPG, ASPG II, ASPGA1, ASPGB1, ASRGL1, At3g16150, colaspase, crasnitin, DiAsp, EcAII, EcAIII, EcaL-ASNase, ECAR-LANS, elspar, ErA, Erwinase, glutaminase-free L-asparaginase, glutamine-(asparagin-)ase, HPA, L-ASNase, L-asparaginase, L-asparaginase 2, L-asparaginase I, L-asparaginase II, L-asparaginase III, L-asparaginase type II, L-asparaginase type III, L-asparaginase-I, L-asparaginase-II, L-asparagine amidohydrolase, L-asparagine amino hydrolase, L-asparagine aminohydrolase, leunase, PfA, PhA, potassium-dependent asparaginase, potassium-independent asparaginase, potassium-independent L-asparaginase, SAMN05216263_10744, SGR ASNase, type I L-asparaginase, type II L-asparaginase, Ypa

ECTree

     3 Hydrolases

         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds

             3.5.1 In linear amides

                3.5.1.1 asparaginase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
43	Activating Compound
18539	AA Sequence
80	Application
1	CAS Registry Number
72	Cloned(Commentary)
30	Crystallization (Commentary)
3522	Disease/ Diagnostics
9	Expression
15	General Information
19	General Stability
301	Inhibitors
59	kcat/KM [mM/s]
3	Ki Value [mM]
258	KM Value [mM]
35	Localization
73	Metals/Ions
145	Molecular Weight [Da]
82	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
548	Organism
9	Pathway
458	PDB ID
101	pH Optimum
36	pH Range
14	pH Stability
13	pI Value
4	Posttranslational Modification
125	Protein Variants
106	Purification (Commentary)
5	Reaction
1	Reaction Type
345	Reference
4	Renatured (Commentary)
79	Source Tissue
114	Specific Activity [micromol/min/mg]
23	Storage Stability
459	Substrates and Products (Substrate)
108	Subunits
209	Synonyms
1	Systematic Name
76	Temperature Optimum [°C]
25	Temperature Range [°C]
96	Temperature Stability [°C]
121	Turnover Number [1/s]

Turnover Number

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Turnover Number on EC 3.5.1.1 - asparaginase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Turnover Number Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

7 - 24

Asn

2 entries

0.8 - 1.98

beta-L-Asp-L-Phe

2 entries

68

D-Asn

Pectobacterium carotovorum

-

37°C

655078

3378

D-asparagine

Thermococcus gammatolerans

C5A6Q4

at pH 8.5 and 85°C

734492

1362

D-glutamine

Thermococcus gammatolerans

C5A6Q4

at pH 8.5 and 85°C

734492

1600

L-Asn

Pectobacterium carotovorum

-

37°C

655078

0.0027 - 5721

L-asparagine

62 entries

0.004 - 450

L-aspartyl-beta-hydroxamate

17 entries

0.001 - 42

L-Gln

15 entries

0.42 - 2048

L-glutamine

14 entries

0.0108

Nalpha-acetyl-Asn

Dickeya chrysanthemi

Q6Q4F3

pH 8, 37°C

669561

4.1

Nalpha-acetyl-L-asparagine

Pectobacterium carotovorum

-

pH 8.0, 25°C

753459

0.0239 - 4.1

succinamic acid

2 entries

additional information

additional information

Escherichia coli

-

turnover numbers for L-aspartyl-beta-hydroxamate with mutant enzyme G11L and G88I are below 0.01 per sec

657311

-

7

Asn

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248A

657311

24

Asn

Escherichia coli

-

pH 7.0, 25°C, wild-type enzyme

657311

0.8

beta-L-Asp-L-Phe

Homo sapiens

Q7L266

wild type enzyme, at pH 7.5 and 37°C

734479

1.98

beta-L-Asp-L-Phe

Cavia porcellus

-

at pH 7.5 and 37°C

731313

0.0027

L-asparagine

Escherichia coli

-

37°C, pH 8.0, wild-type enzyme and mutant enzyme D178P

686454

0.0128

L-asparagine

Dickeya chrysanthemi

Q6Q4F3

pH 8, 37°C

669561

0.025

L-asparagine

Rhizomucor miehei

W0G253

pH 7.0, 45°C

741679

0.22

L-asparagine

Yersinia pseudotuberculosis

Q66CJ2

pH 8.0, 37°C

720973

1.62

L-asparagine

Priestia megaterium

-

wild-type, pH 8.0, 40°C

754219

3.19

L-asparagine

Homo sapiens

Q7L266

wild type enzyme, at pH 7.5 and 37°C

734479

3.95

L-asparagine

Cavia porcellus

-

at pH 7.5 and 37°C

731313

4.65

L-asparagine

Staphylococcus sp.

J9H7D1

at pH 8.6 and 37°C

734465

4.83

L-asparagine

Helicobacter pylori

I0ZIE0

mutant enzyme M121C, at pH 7.5 and 37°C

735004

4.83

L-asparagine

Helicobacter pylori

A0A2X5A091

mutant M121C, pH 7.5, 37°C

755075

6.53

L-asparagine

Helicobacter pylori

I0ZIE0

mutant enzyme Q63E, at pH 7.5 and 37°C

735004

6.53

L-asparagine

Helicobacter pylori

A0A2X5A091

mutant Q63E, pH 7.5, 37°C

755075

7.4

L-asparagine

Helicobacter pylori

I0ZIE0

mutant enzyme T169M, at pH 7.5 and 37°C

735004

7.4

L-asparagine

Helicobacter pylori

A0A2X5A091

mutant T169M, pH 7.5, 37°C

755075

13.95

L-asparagine

Helicobacter pylori

I0ZIE0

mutant enzyme M121C/T169M, at pH 7.5 and 37°C

735004

13.95

L-asparagine

Helicobacter pylori

A0A2X5A091

mutant M121C/T169M, pH 7.5, 37°C

755075

17.98

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 7.4, 37°C, wild-type enzyme

719439

19.2

L-asparagine

Helicobacter pylori

A0A2X5A091

wild-type, pH 7.5, 37°C

755075

19.26

L-asparagine

Helicobacter pylori

B6ZCD8

-

696012

19.26

L-asparagine

Helicobacter pylori

I0ZIE0

wild type enzyme, at pH 7.5 and 37°C

735004

23.8

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 7.4, 37°C, mutant T53Q/K274E

719439

24.5

L-asparagine

Bacillus subtilis

-

mutant S180N/D289T/E260F/E292S, pH not specified in the publication, 37°C

755280

24.9

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 7.4, 37°C, mutant K274E

719439

32.2

L-asparagine

Bacillus subtilis

-

mutant E260F, pH not specified in the publication, 37°C

755280

34.5

L-asparagine

Bacillus subtilis

-

mutant E292S, pH not specified in the publication, 37°C

755280

35.2

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 7.4, 37°C, mutant T53Q

719439

36.8

L-asparagine

Bacillus licheniformis

R4L284

at 37°C and pH 8.0

733138

38.6

L-asparagine

Cavia porcellus

H0W0T5

at pH 7.5 and 37°C

734284

43.2

L-asparagine

Bacillus subtilis

-

mutant D289T, pH not specified in the publication, 37°C

755280

47.9

L-asparagine

Bacillus subtilis

-

mutant S180N, pH not specified in the publication, 37°C

755280

48

L-asparagine

Escherichia coli

P00805

presence of 8 mM Gln, pH 8.0, 37°C

754421

48.5

L-asparagine

Bacillus subtilis

-

wild-type, pH not specified in the publication, 37°C

755280

52.8

L-asparagine

Priestia megaterium

-

mutant V26A/E30G/D181G/V245G/G276D, pH 8.0, 40°C

754219

55.2

L-asparagine

Pectobacterium carotovorum

-

pH 8.0, 25°C

753459

57.7

L-asparagine

Priestia megaterium

-

mutant V26A/E30G/K122N/G276D, pH 8.0, 40°C

754219

58.8

L-asparagine

Escherichia coli

P00805

mutant N24S, pH not specified in the publication, temperature not specified in the publication

755459

59.8

L-asparagine

Escherichia coli

P00805

wild-type, pH not specified in the publication, temperature not specified in the publication

755459

60

L-asparagine

Escherichia coli

P00805

pH 8.0, 37°C

754421

67

L-asparagine

Escherichia coli

P00805

presence of 0.9 mM Gln, pH 8.0, 37°C

754421

72

L-asparagine

Escherichia coli

P00805

presence of 0.3 mM Gln, pH 8.0, 37°C

754421

86.7

L-asparagine

Wolinella succinogenes

P50286

mutant S121P, 37°C, pH not specified in the publication

755467

97.8

L-asparagine

Wolinella succinogenes

P50286

wild-type, 37°C, pH not specified in the publication

755467

106

L-asparagine

Rhizobium etli

Q9RFN5

pH 7.5, 37°C, recombinant enzyme

720213

160

L-asparagine

Pectobacterium carotovorum

-

pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide

720205

160

L-asparagine

Pectobacterium carotovorum

-

pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide

720205

178.6

L-asparagine

Withania somnifera

-

-

699399

199.2

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 8.2, 80°C, mutant K274E

719439

217

L-asparagine

Saccharomyces cerevisiae

-

K0.5 value, pH 8.8, 37°C

755447

246.7

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 8.2, 80°C, mutant T53Q

719439

277.9

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 8.2, 80°C, mutant T53Q/K274E

719439

286.3

L-asparagine

Talaromyces pinophilus

-

pH 8.0, 37°C

754438

523

L-asparagine

Saccharomyces cerevisiae

-

presence of L-glutamine, K0.5 value, pH 8.8, 37°C

755447

565

L-asparagine

Dickeya chrysanthemi

-

pH 8.6, 37°C, wild-type enzyme

728290

573

L-asparagine

Dickeya chrysanthemi

-

pH 8.6, 37°C, mutant enzyme N281D

728290

657

L-asparagine

Dickeya chrysanthemi

-

pH 8.6, 37°C, mutant enzyme N41D

728290

676

L-asparagine

Rhizomucor miehei

W0G253

at pH 7.0 and 45°C

733171

694

L-asparagine

Thermococcus kodakarensis

Q5JHT1

at pH 8.0 and 90°C

734102

798

L-asparagine

Dickeya chrysanthemi

-

pH 8.6, 37°C, mutant enzyme N41D/N281D

728290

888.6

L-asparagine

Pyrococcus furiosus

Q8TZE8

pH 8.2, 80°C, wild-type enzyme

719439

2680

L-asparagine

Bacillus licheniformis

-

in 50 mM Tris-HCl buffer (pH 8.6), at 37°C

735144

4424

L-asparagine

Vibrio cholerae

-

pH 7.0, 37°C

753840

5721

L-asparagine

Thermococcus gammatolerans

C5A6Q4

at pH 8.5 and 85°C

734492

0.004

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme G88A

657311

0.11

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme G11V

657311

0.143

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme Q59G

657311

0.2

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme G57L

657311

0.46

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248D

657311

0.86

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme G57V

657311

2

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme Q59E

657311

9.7

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme Q59A

657311

10.3

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme V27L

657311

10.8

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme V27M

657311

15.4

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme G57A

657311

21

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248Q

657311

23

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248G

657311

26

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248W

657311

27

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248A

657311

29

L-aspartyl-beta-hydroxamate

Escherichia coli

-

pH 7.0, 25°C, wild-type enzyme

657311

450

L-aspartyl-beta-hydroxamate

Pectobacterium carotovorum

-

37°C

655078

0.001

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme Q59A

657311

0.0024

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme Q59E

657311

0.0029

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248A

657311

0.0043

L-Gln

Dickeya chrysanthemi

Q6Q4F3

pH 8.2, 37°C

669561

0.0046

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248G

657311

0.006

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme G57V

657311

0.0068

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248D

657311

0.01

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme G57A

657311

0.01

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme Q59G

657311

0.019

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248E

657311

0.032

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme N248Q

657311

0.032

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme V27M

657311

0.091

L-Gln

Escherichia coli

-

pH 7.0, 25°C, mutant enzyme V27L

657311

0.33

L-Gln

Escherichia coli

-

pH 7.0, 25°C, wild-type enzyme

657311

42

L-Gln

Pectobacterium carotovorum

-

37°C

655078

0.42

L-glutamine

Pectobacterium carotovorum

-

pH 8.0, 25°C

753459

0.51

L-glutamine

Escherichia coli

P00805

wild-type, pH not specified in the publication, temperature not specified in the publication

755459

0.53

L-glutamine

Escherichia coli

P00805

mutant N24S, pH not specified in the publication, temperature not specified in the publication

755459

1.58

L-glutamine

Wolinella succinogenes

P50286

wild-type, 37°C, pH not specified in the publication

755467

2.1

L-glutamine

Helicobacter pylori

A0A2X5A091

wild-type, pH 7.5, 37°C

755075

2.2

L-glutamine

Escherichia coli

P00805

pH 8.0, 37°C

754421

6

L-glutamine

Pectobacterium carotovorum

-

pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide

720205

7.1

L-glutamine

Pectobacterium carotovorum

-

pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide

720205

11.33

L-glutamine

Wolinella succinogenes

P50286

mutant S121P, 37°C, pH not specified in the publication

755467

14.5

L-glutamine

Helicobacter pylori

I0ZIE0

mutant enzyme Q63E, at pH 7.5 and 37°C

735004

14.5

L-glutamine

Helicobacter pylori

A0A2X5A091

mutant Q63E, pH 7.5, 37°C

755075

22.1

L-glutamine

Helicobacter pylori

B6ZCD8

-

696012

22.1

L-glutamine

Helicobacter pylori

I0ZIE0

wild type enzyme, at pH 7.5 and 37°C

735004

2048

L-glutamine

Thermococcus gammatolerans

C5A6Q4

at pH 8.5 and 85°C

734492

0.0239

succinamic acid

Dickeya chrysanthemi

Q6Q4F3

pH 8, 37°C

669561

4.1

succinamic acid

Pectobacterium carotovorum

-

pH 8.0, 25°C

753459