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3.5.1.1: asparaginase

This is an abbreviated version!
For detailed information about asparaginase, go to the full flat file.

Word Map on EC 3.5.1.1

Reaction

L-asparagine
+
H2O
=
L-aspartate
+
NH3

Synonyms

14270 ASNase, alpha-asparaginase, AnsA, ansA3, AnsB, ansZ, Asn, ASNase, ASNase1, ASNase3, ASP1, ASP3, asparaginase, asparaginase II, asparagine amidohydrolase, asparginase II, ASPG, ASPG II, ASPGA1, ASPGB1, ASRGL1, At3g16150, colaspase, crasnitin, DiAsp, EcAII, EcAIII, EcaL-ASNase, ECAR-LANS, elspar, ErA, Erwinase, glutaminase-free L-asparaginase, glutamine-(asparagin-)ase, HPA, L-ASNase, L-asparaginase, L-asparaginase 2, L-asparaginase I, L-asparaginase II, L-asparaginase III, L-asparaginase type II, L-asparaginase type III, L-asparaginase-I, L-asparaginase-II, L-asparagine amidohydrolase, L-asparagine amino hydrolase, L-asparagine aminohydrolase, leunase, PfA, PhA, potassium-dependent asparaginase, potassium-independent asparaginase, potassium-independent L-asparaginase, SAMN05216263_10744, SGR ASNase, type I L-asparaginase, type II L-asparaginase, Ypa

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.1 asparaginase

Temperature Range

Temperature Range on EC 3.5.1.1 - asparaginase

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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 60
20 - 42
-
20°C: about 30% of maximal activity, 42°C: about 35% of maximal activity
20 - 75
the enzyme is active between 20 and 75°C. At temperatures above 37°C, reaction rate declines sharply with the loss of 80% of its original activity at 75°C
30 - 50
-
the enzyme is active at the temperature range of 30-50°C and shows a steep decent in activity above 60°C
30 - 55
-
30°C: about 60% of maximal activity, 55°C: about 55% of maximal activity
30 - 60
30 - 65
more than 50% activity between 30 and 65°C
30 - 70
-
activity range, sharp decrease in activity above 37°C
30 - 75
-
ca. 30 U/ml at 30°C and 75°C
30 - 80
-
immobilized enzyme, 60% of maximal activity at 70°C
32 - 62
-
32°C: about 60% of maximal activity, 62°C: about 85% pf maximal activity
37 - 50
over 90% of activity is retained between 37°C and 50°C. Below 25°C, the enzyme loses most of its activity
41 - 50
Erwinia aroidea
-
41°C: optimum, 50°C: 90% of maximal activity
45
-
about 25% of maximum activity
5 - 45
-
above 45°C sharp decline in activity
55 - 85
-
enzyme incubation for different time periods monitored over a range of 55-85°C shows linear kinetics. From the plot the activation energy is found to be 42.5 kJ/mol*K
57 - 73
over 80% of maximal activity within this range
70 - 100
the enzyme retains more than 50% activity between 70 and 100°C
70 - 95
the enzyme shows more than 75% of relative activity in the range of 70-95°C
77
-
the enzyme follows linear kinetics, even at 77°C
additional information
-
most active at temperatures up to 60°C (pH7)