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Literature summary for 3.5.1.1 extracted from
- Helicobacter pylori L-asparaginase: a promising chemotherapeutic agent (2008), Biochem. Biophys. Res. Commun., 377, 1222-1226.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | chemotherapeutic agent | Helicobacter pylori |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Helicobacter pylori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
L-glutamine | kcat/Km: 0.48 1/mM*s, pH7.5, 37°C | Helicobacter pylori |  |
| additional information | - |
L-asparagine | kcat/Km: 66.41 1/mM*s, pH7.5, 37°C | Helicobacter pylori | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
subunit molecular weight, determined by SDS-PAGE | Helicobacter pylori |
| 140000 | - |
gel filtration | Helicobacter pylori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Helicobacter pylori | B6ZCD8 | CCUG 17874 | - |
| Helicobacter pylori CCUG 17874 | B6ZCD8 | CCUG 17874 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Helicobacter pylori | L-aspartic acid + NH3 | - |
? | |
| L-asparagine + H2O | - |
Helicobacter pylori CCUG 17874 | L-aspartic acid + NH3 | - |
? | |
| L-glutamine + H2O | - |
Helicobacter pylori | L-glutamic acid + NH3 | - |
? | |
| L-glutamine + H2O | - |
Helicobacter pylori CCUG 17874 | L-glutamic acid + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 37000, SDS-PAGE | Helicobacter pylori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-asparaginase | type II | Helicobacter pylori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
absolutely stable up to 50°C (10 min incubation time), 50% activity after 10 min at 53°C | Helicobacter pylori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.26 | - |
L-asparagine | - |
Helicobacter pylori | |
| 22.1 | - |
L-glutamine | - |
Helicobacter pylori | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | broad optimum for asparaginase activity | Helicobacter pylori |
| 7.5 | - |
glutaminase activity | Helicobacter pylori |
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Release 2023.1 (January 2023)
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