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Literature summary for 3.5.1.1 extracted from
- Assessment of physical process conditions for enhanced Production of novel glutaminase-free L-asparaginase from Pectobacterium carotovorum MTCC 1428 (2011), Appl. Biochem. Biotechnol., 163, 327-337.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | statistically based experimental design to maximize the production of glutaminase-free L-asparaginase. The individual optimum levels of initial pH of the medium, temperature, rpm of shaking incubator, and inoculum size are found to be 6.90, 29.8°C, 157 rpm, and 2.61% (v/v), respectively, for the production of L-asparaginase. After physical process parameters optimization, the production and productivity of L-asparaginase is enhanced by 26.39% (specific activity) and 10.19%, respectively. Maximization of L-asparaginase production is achieved at 12 h under optimal levels of physical process parameters in shake flask level | Pectobacterium carotovorum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Pectobacterium carotovorum | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36100 | - |
4 * 36100, MALDI-TOF mass spectrophotometry analysis | Pectobacterium carotovorum |
| 144400 | - |
native PAGE, gel filtration, and MALDI-TOF mass spectrophotometry analysis | Pectobacterium carotovorum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pectobacterium carotovorum | - |
MTCC 1428 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| three steps purification process | Pectobacterium carotovorum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
no glutaminase activity is observed | Pectobacterium carotovorum |
| additional information | - |
the production and productivity of L-asparaginase is enhanced by 26.39% (specific activity) and 10.19%, respectively. The individual optimum levels of initial pH of the medium, temperature, rpm of shaking incubator, and inoculum size are found to be 6.90, 29.8°C, 157 rpm, and 2.61% (v/v), respectively, for the production of L-asparaginase. | Pectobacterium carotovorum |
| 3.35 | - |
under unoptimized levels of medium components of the production of intracelluar L-asparaginase, pH 8.6, 37°C | Pectobacterium carotovorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | - |
Pectobacterium carotovorum | L-aspartate + NH3 | - |
? |  |
| additional information | no glutaminase activity is observed | Pectobacterium carotovorum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 36100, MALDI-TOF mass spectrophotometry analysis | Pectobacterium carotovorum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutaminase-free L-asparaginase | - |
Pectobacterium carotovorum |
| L-asparaginase | - |
Pectobacterium carotovorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pectobacterium carotovorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Pectobacterium carotovorum |
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Release 2023.1 (January 2023)
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