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Literature summary for 3.5.1.1 extracted from

  • Huang, L.; Liu, Y.; Sun, Y.; Yan, Q.; Jiang, Z.
    Biochemical characterization of a novel L-Asparaginase with low glutaminase activity from Rhizomucor miehei and its application in food safety and leukemia treatment (2014), Appl. Environ. Microbiol., 80, 1561-1569.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.1

Application

Application Comment Organism
food industry reduction of acrylamide level in biscuits and bread Rhizomucor miehei
medicine the recombinant enzyme potentiate a lectin's induction of leukemic K562 cell apoptosis Rhizomucor miehei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rhizomucor miehei

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 55% residual activity at 2 mM Rhizomucor miehei
Ag+ 42% residual activity at 2 mM Rhizomucor miehei
Ba2+ 30% residual activity at 2 mM Rhizomucor miehei
Co2+ 69% residual activity at 2 mM Rhizomucor miehei
Cr2+ 37% residual activity at 2 mM Rhizomucor miehei
Cu2+ 13% residual activity at 2 mM Rhizomucor miehei
Fe3+ 41% residual activity at 2 mM Rhizomucor miehei
Hg2+ 24% residual activity at 2 mM Rhizomucor miehei
K+ 92% residual activity at 2 mM Rhizomucor miehei
Mg2+ 28% residual activity at 2 mM Rhizomucor miehei
Na+ 91% residual activity at 2 mM Rhizomucor miehei
Ni2+ 85% residual activity at 2 mM Rhizomucor miehei
SDS 56% residual activity at 2 mM Rhizomucor miehei
Sn2+ 87% residual activity at 2 mM Rhizomucor miehei
Zn2+ 17% residual activity at 2 mM Rhizomucor miehei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 144% activity at 2 mM Rhizomucor miehei
Mn2+ 183% activity at 2 mM Rhizomucor miehei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
 2 * 72000, SDS-PAGE Rhizomucor miehei
75316
-
 2 * 75316, calculated from amino acid sequence Rhizomucor miehei
133700
-
 gel filtration Rhizomucor miehei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Rhizomucor miehei highest specificity for L-asparagine L-aspartate + NH3
-
 ?
L-asparagine + H2O Rhizomucor miehei CAU432 highest specificity for L-asparagine L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Rhizomucor miehei W0G253
-
-
Rhizomucor miehei CAU432 W0G253
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IDA column chromatography Rhizomucor miehei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7797
-
 crude enzyme, at 45°C in 50 mM phosphate buffer (pH 7.0) Rhizomucor miehei
1.985
-
 after 2.6fold purification, at 45°C in 50 mM phosphate buffer (pH 7.0) Rhizomucor miehei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-asparagine + H2O negligible activity Rhizomucor miehei D-aspartate + NH3
-
 ?
D-asparagine + H2O negligible activity Rhizomucor miehei CAU432 D-aspartate + NH3
-
 ?
L-asparagine + H2O highest specificity for L-asparagine Rhizomucor miehei L-aspartate + NH3
-
 ?
L-asparagine + H2O highest specificity for L-asparagine Rhizomucor miehei CAU432 L-aspartate + NH3
-
 ?
L-glutamine + H2O negligible activity Rhizomucor miehei L-glutamate + NH3
-
 ?
L-glutamine + H2O negligible activity Rhizomucor miehei CAU432 L-glutamate + NH3
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 72000, SDS-PAGE Rhizomucor miehei
homodimer 2 * 75316, calculated from amino acid sequence Rhizomucor miehei

Synonyms

Synonyms Comment Organism
ASNase
-
 Rhizomucor miehei
L-asparaginase
-
 Rhizomucor miehei
L-asparagine amidohydrolase
-
 Rhizomucor miehei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Rhizomucor miehei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 more than 50% activity between 30 and 65°C Rhizomucor miehei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 the recombinant enzyme is stable at 45°C for 30 min Rhizomucor miehei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
676
-
 L-asparagine at pH 7.0 and 45°C Rhizomucor miehei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rhizomucor miehei

pH Range

pH Minimum pH Maximum Comment Organism
4 8 more than 80% activity between pH 4.0 and 8.0 Rhizomucor miehei

pI Value

Organism Comment pI Value Maximum pI Value
Rhizomucor miehei calculated from amino acid sequence
-
 6.8