Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycine | the enzyme is activated by incubating for 4 days in a 2.0 M glycine (pH 9.0) solution at room temperature | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) C41 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 2.2-2.5 M sodium malonate (pH 7.0) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T168S | the mutant shows no self-cleavage | Homo sapiens |
T186V | the inactive mutant shows partial (45%) self-cleavage | Homo sapiens |
T219A | the inactive mutant shows a complete, albeit slow self-cleavage | Homo sapiens |
T219V | the inactive mutant displays partial (50%) self-cleavage | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
beta-L-Asp-L-Phe | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
2.09 | - |
L-asparagine | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE | Homo sapiens |
20000 | - |
1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE | Homo sapiens |
37000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Homo sapiens | - |
L-aspartate + NH3 | - |
? | |
additional information | Homo sapiens | to become enzymatically active, ASNase3 must undergo autocleavage between residues Gly167 and Thr168 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q7L266 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-L-Asp-L-Phe + H2O | - |
Homo sapiens | ? | - |
? | |
L-asparagine + H2O | - |
Homo sapiens | L-aspartate + NH3 | - |
? | |
additional information | to become enzymatically active, ASNase3 must undergo autocleavage between residues Gly167 and Thr168 | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 20000 + 1 * 12000, uncleaved inactive form, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
ASNase3 | - |
Homo sapiens |
L-asparaginase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
beta-L-Asp-L-Phe | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
3.19 | - |
L-asparagine | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.52 | - |
L-asparagine | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
6.66 | - |
beta-L-Asp-L-Phe | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |