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Literature summary for 3.5.1.1 extracted from

  • Wink, P.; Bogdawa, H.; Renard, G.; Chies, J.; Basso, L.; Santos, D.
    Comparison between two Erwinia carotovora L-asparaginase II constructions: cloning, Heterologous expression, purification, and kinetic characterization (2010), J. Microb. Biochem. Technol., 2, 13-19.
No PubMed abstract available
Search for more literature for 3.5.1.1

Application

Application Comment Organism
medicine L-asparaginase II from Erwinia carotovora may represent an important alternative therapy in the treatment of acute childhood lymphoblastic leukaemia, despite its promising lower glutaminase activity than Escherichia coli and Erwinia chrysanthemi L-asparaginases II Pectobacterium carotovorum

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of L-asparaginase II with signal peptide, AspSP, in Escherichia coli strain BL21(DE3) in the periplasmic space and extracellular, and of L-asparaginase II without signal peptide, AspMP, in Escherichia coli strain C41(DE3) in the cytoplasm Pectobacterium carotovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten steady-state kinetics, overview Pectobacterium carotovorum
0.16
-
 L-asparagine pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide Pectobacterium carotovorum
1.1
-
 L-asparagine pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide Pectobacterium carotovorum
2.6
-
 L-glutamine pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide Pectobacterium carotovorum
4.4
-
 L-glutamine pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34500
-
 x * 34500, about, sequence calculation, x * 39000, SDS-PAGE Pectobacterium carotovorum
39000
-
 x * 34500, about, sequence calculation, x * 39000, SDS-PAGE Pectobacterium carotovorum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Pectobacterium carotovorum
-
 L-aspartate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant L-asparaginase II with signal peptide, AspSP, by cation exchange chromatography and gel filtration, and recombinant L-asparaginase II without signal peptide, AspMP, by a single-step cation exchange chromatography, to homogeneity Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
208.1
-
 purified AspMP, enzyme without signal peptide, pH 8.0, 37°C Pectobacterium carotovorum
237.6
-
 purified AspSP, enzyme with signal peptide, pH 8.0, 37°C Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-asparagine + H2O
-
 Pectobacterium carotovorum L-aspartate + NH3
-
 ?
L-glutamine + H2O
-
 Pectobacterium carotovorum L-glutamate + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 34500, about, sequence calculation, x * 39000, SDS-PAGE Pectobacterium carotovorum

Synonyms

Synonyms Comment Organism
L-asparaginase II
-
 Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pectobacterium carotovorum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6
-
 L-glutamine pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide Pectobacterium carotovorum
7.1
-
 L-glutamine pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide Pectobacterium carotovorum
160
-
 L-asparagine pH 8.0, 37°C, recombinant AspMP, L-asparaginase II without signal peptide Pectobacterium carotovorum
160
-
 L-asparagine pH 8.0, 37°C, recombinant AspSP, L-asparaginase II with signal peptide Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pectobacterium carotovorum