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3.5.1.1: asparaginase

This is an abbreviated version!
For detailed information about asparaginase, go to the full flat file.

Word Map on EC 3.5.1.1

Reaction

L-asparagine
+
H2O
=
L-aspartate
 +
NH3

Synonyms

14270 ASNase, alpha-asparaginase, AnsA, ansA3, AnsB, ansZ, Asn, ASNase, ASNase1, ASNase3, ASP1, ASP3, asparaginase, asparaginase II, asparagine amidohydrolase, asparginase II, ASPG, ASPG II, ASPGA1, ASPGB1, ASRGL1, At3g16150, colaspase, crasnitin, DiAsp, EcAII, EcAIII, EcaL-ASNase, ECAR-LANS, elspar, ErA, Erwinase, glutaminase-free L-asparaginase, glutamine-(asparagin-)ase, HPA, L-ASNase, L-asparaginase, L-asparaginase 2, L-asparaginase I, L-asparaginase II, L-asparaginase III, L-asparaginase type II, L-asparaginase type III, L-asparaginase-I, L-asparaginase-II, L-asparagine amidohydrolase, L-asparagine amino hydrolase, L-asparagine aminohydrolase, leunase, PfA, PhA, potassium-dependent asparaginase, potassium-independent asparaginase, potassium-independent L-asparaginase, SAMN05216263_10744, SGR ASNase, type I L-asparaginase, type II L-asparaginase, Ypa

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.1 asparaginase

Cloned

Cloned on EC 3.5.1.1 - asparaginase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli
-
cloned and expressed in Escherichia coli BL21 (DE3) cells
expressed in Bacillus subtilis strain 168
-
expressed in Escherichia coli BL21(DE3) C41 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli BLR(DE3) cells
expressed in Escherichia coli JM109 cells
expressed in Pichia pastoris under the control of the AOX1 gene promoter. High cell density cultures performed with Pichia pastoris harbouring the ASP3 gene using a 2 l instrumented bioreactor, where biomass concentration reached 107 g/l, results in a dramatic increase in volumetric yield (85600 U/l) and global volumetric productivity (1083 U/l)
-
expressed in Pichia pastoris, haboring the ASP3 gene of asparaginase from Saccharomyces cervisiae
-
expression in Escherichia coli
expression in Escherichia coli as a fusion protein with a polyhistidine tail
-
expression in Escherichia coli as N-terminal His-tagged protein
expression in Escherichia coli BL21(DE3)pLysS
expression in Escherichia coli GJ1158. The enzyme is produced intracellularly. There is a marked improvement in the activity of L-asparaginase, obtained from recombinant cells, which is 5fold higher than the native enzyme
-
expression in Escherichia coli strain BL21(DE3)
expression of His-tagged enzyme
-
expression of His-tagged mutant enzymes in Escherichia coli strain Rosetta (DE3)
expression of mutant enzymes in Escherichia coli
-
expression of the mutant chimeric enzyme in the periplasm of strain BL21(DE3)
-
expression of the N-terminally His-tagged enzyme fused to the pelB leader sequence under control of the T7lac promoter in strain BLR(DE3), the recombinant enzyme is secreted to the cell culture medium
-
gene ansA, phylogenetic analysis, expression of His-tagged AnsA in Escherichia coli strain BL21(DE3)
gene ansB, cloning in Escherichia coli strain DH5alpha and expression as His6-tagged protein in Escherichia coli strain BL21(DE3)
-
gene ansB, expression in Escherichia coli strain BL21 (DE3)
gene ansB, expression in Escherichia coli strain BL21 (DE3), method evaluation, overview
gene PH0066, expression in Escherichia coli strain BL21
-
grown by the hanging-drop vapor-diffusion method from protein solutions in a HEPES buffer (pH 6.5) and PEG MME 5000 solutions in a cacodylate buffer (pH 6.5) as the precipitant. Three-dimensional X-ray diffraction data are collected up to 3 A resolution from one crystal at room temperature
-
mutants containing Asn to Asp changes
-
recombinant expression of L-asparaginase II with signal peptide, AspSP, in Escherichia coli strain BL21(DE3) in the periplasmic space and extracellular, and of L-asparaginase II without signal peptide, AspMP, in Escherichia coli strain C41(DE3) in the cytoplasm
-
Streptomyces library screening and cloning, overexpression without artificial signal peptide in Streptomyces lividans using a hyperexpression vector pTONA5a is not successful
Streptomyces library screening, cloning, and functional overexpression without artificial signal peptide in Streptomyces lividans using a hyperexpression vector pTONA5a