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3.5.1.1: asparaginase

This is an abbreviated version!
For detailed information about asparaginase, go to the full flat file.

Word Map on EC 3.5.1.1

Reaction

L-asparagine
+
H2O
=
L-aspartate
+
NH3

Synonyms

14270 ASNase, alpha-asparaginase, AnsA, ansA3, AnsB, ansZ, Asn, ASNase, ASNase1, ASNase3, ASP1, ASP3, asparaginase, asparaginase II, asparagine amidohydrolase, asparginase II, ASPG, ASPG II, ASPGA1, ASPGB1, ASRGL1, At3g16150, colaspase, crasnitin, DiAsp, EcAII, EcAIII, EcaL-ASNase, ECAR-LANS, elspar, ErA, Erwinase, glutaminase-free L-asparaginase, glutamine-(asparagin-)ase, HPA, L-ASNase, L-asparaginase, L-asparaginase 2, L-asparaginase I, L-asparaginase II, L-asparaginase III, L-asparaginase type II, L-asparaginase type III, L-asparaginase-I, L-asparaginase-II, L-asparagine amidohydrolase, L-asparagine amino hydrolase, L-asparagine aminohydrolase, leunase, PfA, PhA, potassium-dependent asparaginase, potassium-independent asparaginase, potassium-independent L-asparaginase, SAMN05216263_10744, SGR ASNase, type I L-asparaginase, type II L-asparaginase, Ypa

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.1 asparaginase

Storage Stability

Storage Stability on EC 3.5.1.1 - asparaginase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 3 weeks, lyophilized enzyme, no loss of activity
-20°C, 30% glycerol, stable for 1 month
-20°C, 50 mM Tris-HCl (pH 8.6), 30 days, almost no loss of activity
-
-20°C, less than 10% loss of activity after 3 months
-
-20°C, little loss of activity after prolonged storage
-
-20°C, stable
-
-20°C, stable for 1 month
-
-20°C, stable for 20 days
-
-20°C, stable for at least 3 months
-
-40°°C, 1000U/ml in plasma solution, 1 year, no decline in activity
-
-70°C to -20°C, purified enzyme, pH 5.0-9.0, several weeks, no loss of activity
0°C, 20 mM Tris, pH 7.5, 2 mM dithiothreitol, 1 M KCl, 8 h, the enzyme starts rapidly losing activity
22°C, 24 h, lyophilized enzyme, no loss of activity
22°C, pH 9.2, 4 months, the enzyme retains about 90% of enzyme activity
-
4°C, after 3 weeks, the residual activity of wild-type enzyme decreases more rapidly compared to the D178P. After 5 weeks, wild-type enzyme loses 80% of its activity, the mutant enzyme D178P loses 58% of its activity
-
4°C, pH 6.0, 0.1 M citrate buffer, stable for several weeks
-
4°C, stable for up to 4 days
-
5°C lyphilyzed enzyme, stable upon storage for at least several weeks
-
5°C, as suspension in 3.5 M ammonium sulfate, stable for 1 year
-
5°C, sterile enzyme solution with high specific activity, several weeks, no loss of activity
-
stable in the frozen state for 6 months or longer
-
the crude asparaginase preparations are stable upon storage at -18°C for several months
-