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Literature summary for 3.5.1.1 extracted from

  • Schalk, A.M.; Lavie, A.
    Structural and kinetic characterization of guinea pig L-asparaginase type III (2014), Biochemistry, 53, 2318-2328.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
glycine glycine stimulates self-cleavage in a dose-dependent manner Cavia porcellus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cavia porcellus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M MES at pH 6.0, and 13-15% (w/v) PEG 3350 Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.41
-
 beta-L-Asp-L-Phe at pH 7.5 and 37°C Cavia porcellus
2.24
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
 1 * 20000 + 1 * 15000, active, cleaved enzyme Cavia porcellus
20000
-
 1 * 20000 + 1 * 15000, active, cleaved enzyme Cavia porcellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Cavia porcellus
-
 L-aspartate + NH3
-
 ?
additional information Cavia porcellus the enzyme requires autocleavage to become active ?
-
 ?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP Ni Sepharose column chromatography and Superdex 200 gel filtration Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-L-Asp-L-Phe + H2O
-
 Cavia porcellus ?
-
 ?
L-asparagine + H2O
-
 Cavia porcellus L-aspartate + NH3
-
 ?
additional information the enzyme requires autocleavage to become active Cavia porcellus ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 20000 + 1 * 15000, active, cleaved enzyme Cavia porcellus

Synonyms

Synonyms Comment Organism
ASNase3
-
 Cavia porcellus
L-asparaginase type III
-
 Cavia porcellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.98
-
 beta-L-Asp-L-Phe at pH 7.5 and 37°C Cavia porcellus
3.95
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus

General Information

General Information Comment Organism
additional information ASNase3 is not the enzyme responsible for the antitumor effects of guinea pig serum Cavia porcellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.76
-
 L-asparagine at pH 7.5 and 37°C Cavia porcellus
4.83
-
 beta-L-Asp-L-Phe at pH 7.5 and 37°C Cavia porcellus