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Literature summary for 3.5.1.1 extracted from
- L-Asparaginase of Thermus thermophilus: purification, properties and identification of essential amino acids for its catalytic activity (2001), Mol. Cell. Biochem., 216, 93-101.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Asn | competitive | Thermus thermophilus |  |
| diethyldicarbonate | - |
Thermus thermophilus | |
| iodoacetamide | at pH 6.0 protection by substrate, at pH 8.0 no protection by substrate | Thermus thermophilus | |
| L-Asp | competitive | Thermus thermophilus | |
| N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinline | no effect up to 1 mM: Mg2+, Mn2+ and Ca2+ | Thermus thermophilus | |
| NEM | - |
Thermus thermophilus | |
| p-hydroxymercuribenzoate | - |
Thermus thermophilus | |
| Phenylglyoxal | protection by substrate | Thermus thermophilus | |
| PMSF | partial inactivation | Thermus thermophilus | |
| Zn2+ | 40% inhibition by 0.5 mM, 60% inhibition by 1.0 mM | Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
L-Asn | pH 9.2, 60°C | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
6 * 33000, SDS-PAGE | Thermus thermophilus |
| 200000 | - |
gel filtration | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 840 | - |
- |
Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Asn + H2O | - |
Thermus thermophilus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 33000, SDS-PAGE | Thermus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.2 | - |
- |
Thermus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | 10.4 | pH 8.2: about 60% of maximal activity, pH 10.4: about 60% of maximal activity | Thermus thermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermus thermophilus | isoelectric focusing, pH-range 3-10 | - |
6 |
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