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Literature summary for 3.5.1.1 extracted from
- Purification and properties of asparaginase from Lupinus arboreus and Lupinus angustifolius (1981), Arch. Biochem. Biophys., 208, 49-58.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-Cyano-L-Ala | - |
Lupinus arboreus |  |
| 5-Diazo-4-oxo-L-norvaline | - |
Lupinus arboreus | |
| Cys | - |
Lupinus arboreus | |
| D-Asn | - |
Lupinus arboreus | |
| D-Asp | - |
Lupinus arboreus | |
| DL-aspartyl hydroxamate | - |
Lupinus arboreus | |
| ethylenediamine | weak | Lupinus arboreus | |
| Gly | - |
Lupinus arboreus | |
| Gly methyl ester | - |
Lupinus arboreus | |
| Gly-Gly | - |
Lupinus arboreus | |
| L-Asp | - |
Lupinus arboreus | |
| Succinamide | weak | Lupinus arboreus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.6 | - |
L-asparagine | - |
Lupinus arboreus | |
| 7 | - |
L-asparagine | - |
Lupinus angustifolius | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Lupinus angustifolius |
| 75000 | - |
gel filtration | Lupinus arboreus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lupinus angustifolius | - |
- |
- |
| Lupinus arboreus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lupinus angustifolius |
- |
Lupinus arboreus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | - |
Lupinus arboreus | - |
| leaf | - |
Lupinus arboreus | - |
| nodule | developing | Lupinus angustifolius | - |
| root | tips | Lupinus angustifolius | - |
| root | tips | Lupinus arboreus | - |
| seed | - |
Lupinus angustifolius | - |
| seed | - |
Lupinus arboreus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.97 | - |
- |
Lupinus arboreus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 30% glycerol, stable for 1 month | Lupinus angustifolius |
| -20°C, 30% glycerol, stable for 1 month | Lupinus arboreus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-aspartyl hydroxamate + H2O | 66% of the activity with L-Asn | Lupinus arboreus | DL-Asp + hydroxylamine | - |
? | |
| L-Asn + H2O | - |
Lupinus angustifolius | L-Asp + NH3 | - |
? | |
| L-Asn + H2O | - |
Lupinus arboreus | L-Asp + NH3 | - |
? | |
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