General Stability | Organism |
---|---|
reversibly dissociated by urea | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Diazo-4-oxo-L-norvaline | - |
Escherichia coli | |
L-Asn | inhibits hydrolysis of diazo-4-oxo-L-norvaline | Escherichia coli | |
L-Asp | product inhibition at pH 8.5 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
Asn | - |
Acinetobacter calcoaceticus | |
0.0058 | - |
Gln | - |
Acinetobacter calcoaceticus | |
0.0095 | - |
diazo-4-oxo-L-norvaline | - |
Escherichia coli | |
0.01 | - |
L-asparagine | - |
Pectobacterium carotovorum | |
0.0115 | - |
Asn | - |
Escherichia coli | |
0.0125 | - |
L-asparagine | - |
Escherichia coli | |
0.026 | - |
L-asparagine | - |
Proteus vulgaris | |
0.03 | - |
Asn | - |
Cupriavidus necator | |
0.04 | - |
L-Gln | - |
Cupriavidus necator | |
0.06 | - |
Asn | - |
Gallus gallus | |
0.072 | - |
L-asparagine | - |
Cavia porcellus | |
0.1 | - |
L-asparagine | - |
Serratia marcescens | |
0.5 | - |
L-asparagine | - |
Fusarium tricinctum | |
1.1 | - |
Gln | - |
Pectobacterium carotovorum | |
3 | - |
L-Asn | - |
Acinetobacter calcoaceticus | |
3 | - |
L-Asn | - |
Erwinia aroidea | |
4.7 | - |
Asn | - |
Weizmannia coagulans | |
6.26 | - |
Gln | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
85000 | - |
sucrose density gradient centrifugation | Weizmannia coagulans |
97000 | - |
gel filtration | Acinetobacter glutaminasificans |
100000 | 120000 | gel filtration | Escherichia coli |
118000 | - |
gel filtration | Pseudomonas sp. |
122000 | - |
ultracentrifugation | Pseudomonas sp. |
130000 | - |
- |
Acinetobacter calcoaceticus |
130000 | - |
ultracentrifugation | Escherichia coli |
130000 | - |
ultracentrifugation | Acinetobacter glutaminasificans |
133000 | - |
gel filtration | Cavia porcellus |
135000 | - |
ultracentrifugation | Pectobacterium carotovorum |
138000 | - |
ultracentrifugation | Cavia porcellus |
147000 | - |
ultracentrifugation | Serratia marcescens |
150000 | - |
sucrose density gradient centrifugation | Serratia marcescens |
150000 | - |
ultracentrifugation | Cupriavidus necator |
306000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Acinetobacter glutaminasificans | - |
- |
- |
Aspergillus niger | - |
- |
- |
Aspergillus oryzae | - |
- |
- |
Aspergillus terreus | - |
- |
- |
Azotobacter agilis | - |
- |
- |
Brucella abortus | - |
- |
- |
Cavia porcellus | - |
- |
- |
Clostridium cadaveris | - |
- |
- |
Cupriavidus necator | - |
- |
- |
Erwinia aroidea | - |
- |
- |
Escherichia coli | - |
K-12 | - |
Escherichia coli | - |
EC-I | - |
Escherichia coli EC-I | - |
EC-I | - |
Fusarium tricinctum | - |
- |
- |
Gallus gallus | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Lablab purpureus | - |
- |
- |
Lupinus luteus | - |
- |
- |
Mycobacterium avium | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis variant bovis | - |
- |
- |
Mycolicibacterium phlei | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Neurospora crassa | - |
- |
- |
Pectobacterium carotovorum | - |
- |
- |
Platyrrhini | - |
new world | - |
Proteus vulgaris | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas sp. | - |
P-210 | - |
Pseudomonas sp. | - |
GG13 | - |
Pseudomonas sp. GG13 | - |
GG13 | - |
Pseudomonas sp. P-210 | - |
P-210 | - |
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Salmonella typhosa | - |
- |
- |
Serratia marcescens | - |
- |
- |
Staphylococcus sp. | - |
- |
- |
Streptomyces griseus | - |
- |
- |
Weizmannia coagulans | - |
- |
- |
[Pseudomonas] boreopolis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
liver | - |
Cavia porcellus | - |
serum | - |
Cavia porcellus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Gly-L-Asn + H2O | - |
Pectobacterium carotovorum | benzyloxycarbonyl-Gly-L-Asp | - |
? | |
beta-aspartoethylamide + H2O | at 0.4% of the activity with L-Asn | Pectobacterium carotovorum | ? | - |
? | |
beta-aspartomethylamide + H2O | at 0.5% of the activity with L-Asn | Pectobacterium carotovorum | L-Asp + ethylamine | - |
? | |
beta-aspartopropylamide + H2O | at 0.7% of the activity with L-Asn | Pectobacterium carotovorum | ? | - |
? | |
beta-cyano-L-Ala + H2O | beta-cyano-L-Ala | Cavia porcellus | ? | - |
? | |
beta-cyano-L-Ala + H2O | beta-cyano-L-Ala | Escherichia coli | ? | - |
? | |
beta-cyano-L-Ala + H2O | beta-cyano-L-Ala | Pectobacterium carotovorum | ? | - |
? | |
beta-cyano-L-Ala + H2O | at 5.7% of the activity with L-Asn | Pectobacterium carotovorum | ? | - |
? | |
beta-cyano-L-Ala + H2O | at 2.4% of the activity with L-Asn | Escherichia coli | ? | - |
? | |
beta-cyano-L-Ala + H2O | beta-cyano-L-Ala | Escherichia coli EC-I | ? | - |
? | |
beta-cyano-L-Ala + H2O | at 2.4% of the activity with L-Asn | Escherichia coli EC-I | ? | - |
? | |
D-Asn + H2O | - |
Cavia porcellus | D-Asp + NH3 | - |
? | |
D-Asn + H2O | - |
Proteus vulgaris | D-Asp + NH3 | - |
? | |
D-Asn + H2O | - |
Weizmannia coagulans | D-Asp + NH3 | - |
? | |
D-Asn + H2O | at 5% of the activity with L-Asn | Escherichia coli | D-Asp + NH3 | - |
? | |
D-Asn + H2O | at 5% of the activity with L-Asn | Acinetobacter calcoaceticus | D-Asp + NH3 | - |
? | |
D-Asn + H2O | at 5% of the activity with L-Asn | Pectobacterium carotovorum | D-Asp + NH3 | - |
? | |
D-Asn + H2O | at 5% of the activity with L-Asn | Escherichia coli EC-I | D-Asp + NH3 | - |
? | |
diazo-4-oxo-L-norvaline + H2O | - |
Escherichia coli | 5-hydroxy-4-oxo-L-norvaline + NH3 | - |
? | |
DL-Ala-DL-Asn + H2O | - |
Pectobacterium carotovorum | DL-Ala-DL-Asn + DL-Ala-DL-Asp | 75% Ala-Asp + 25% Ala-Asn | ? | |
DL-aspartyl hydroxamate + H2O | - |
Pectobacterium carotovorum | DL-Asp + hydroxylamine | - |
? | |
Gly-D-Asn + H2O | - |
Pectobacterium carotovorum | Gly-D-Asp + NH3 | - |
? | |
Gly-L-Asn + H2O | - |
Pectobacterium carotovorum | Gly-L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Gallus gallus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Cavia porcellus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Escherichia coli | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Rattus norvegicus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Saccharomyces cerevisiae | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Mycolicibacterium smegmatis | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Lupinus luteus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Geobacillus stearothermophilus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Neurospora crassa | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Aspergillus niger | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Pseudomonas fluorescens | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Pseudomonas sp. | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Acinetobacter calcoaceticus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Serratia marcescens | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Brucella abortus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Mycolicibacterium phlei | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Mycobacterium avium | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Proteus vulgaris | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Mycobacterium tuberculosis | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Platyrrhini | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Weizmannia coagulans | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Staphylococcus sp. | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Cupriavidus necator | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Streptomyces griseus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Pectobacterium carotovorum | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Aspergillus oryzae | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Aspergillus terreus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Mycobacterium tuberculosis variant bovis | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Erwinia aroidea | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Azotobacter agilis | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Fusarium tricinctum | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Clostridium cadaveris | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Salmonella typhosa | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Lablab purpureus | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Acinetobacter glutaminasificans | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
[Pseudomonas] boreopolis | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Escherichia coli EC-I | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Pseudomonas sp. P-210 | L-Asp + NH3 | - |
? | |
L-Asn + H2O | - |
Pseudomonas sp. GG13 | L-Asp + NH3 | - |
? | |
L-Gln + H2O | - |
Acinetobacter calcoaceticus | L-Glu + NH3 | - |
? | |
L-Gln + H2O | - |
Proteus vulgaris | L-Glu + NH3 | - |
? | |
L-Gln + H2O | at 9% of the activity with L-Asn | Pectobacterium carotovorum | L-Glu + NH3 | - |
? | |
L-Gln + H2O | at 4% of the activity with L-asparagine | Escherichia coli | L-Glu + NH3 | - |
? | |
L-Gln + H2O | at 4% of the activity with L-asparagine | Escherichia coli EC-I | L-Glu + NH3 | - |
? | |
L-Gln + H2O | - |
Cupriavidus necator | ? | - |
? | |
L-Gln-L-Asn + H2O | - |
Pectobacterium carotovorum | L-Gln-L-Asp + NH3 | - |
? | |
L-Glu-L-Asn + H2O | - |
Pectobacterium carotovorum | L-Glu-L-Asp + NH3 | - |
? | |
succinamic acid + H2O | at 20% of the activity with L-Asn | Pectobacterium carotovorum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | - |
Escherichia coli |