Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Lipid | activates | Tetrahymena pyriformis |
Application | Comment | Organism |
---|---|---|
analysis | direct measurement of L-asparagine in human plasma samples through the use of Escherichia coli Lasparaginase in the soluble form is a major clinical application of this system | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Lupinus angustifolius |
- |
Lupinus arboreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Rhodotorula mucilaginosa | |
Pb2+ | - |
Rhodotorula mucilaginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-asparagine | - |
Mycolicibacterium phlei | |
2.8 | - |
L-asparagine | - |
Erwinia sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Cyberlindnera jadinii | - |
- |
extracellular | - |
Rhodotorula toruloides | - |
- |
intracellular | - |
Enterobacter cloacae | 5622 | - |
microsome | - |
Tetrahymena pyriformis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Rhodotorula mucilaginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE | Thermus thermophilus |
120000 | - |
- |
Sphagnum fallax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora | ? | - |
? | |
additional information | Pectobacterium carotovorum | L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aliivibrio fischeri | - |
- |
- |
Aspergillus niger | - |
- |
- |
Aspergillus tamarii | - |
- |
- |
Aspergillus terreus | - |
- |
- |
Cyberlindnera jadinii | - |
- |
- |
Enterobacter cloacae | - |
- |
- |
Erwinia aroidea | - |
NRR LB-138 | - |
Erwinia aroidea NRR LB-138 | - |
NRR LB-138 | - |
Erwinia sp. | - |
- |
- |
Escherichia coli | - |
strain W and strain K-12 | - |
Lupinus angustifolius | - |
- |
- |
Lupinus arboreus | - |
- |
- |
Mycolicibacterium phlei | - |
- |
- |
Nocardia asteroides | - |
- |
- |
Pectobacterium carotovorum | - |
- |
- |
Photobacterium leiognathi | - |
- |
- |
Photobacterium phosphoreum | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas aeruginosa 50071 | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Rhodotorula mucilaginosa | - |
- |
- |
Rhodotorula toruloides | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | P38986 | - |
- |
Serratia marcescens | - |
- |
- |
Sphagnum fallax | - |
- |
- |
Staphylococcus sp. | - |
- |
- |
Tetrahymena pyriformis | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Vibrio harveyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cyberlindnera jadinii |
- |
Thermus thermophilus |
- |
Pseudomonas aeruginosa |
- |
Mycolicibacterium phlei |
- |
Sphagnum fallax |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition | carbon sources such as sucrose, maltose, galactose, lactose, mannitol and mannose inhibit enzyme production. Exogenous cAMP in presence of carbon sources stimulates L-asparaginase enzyme production | Staphylococcus sp. | - |
culture condition | high L-asparaginase activity is found in cells cultured on L-fructose, D-galactose, sucrose or maltose, and in cells cultured on L-asparagine as the sole nitrogen source | Enterobacter cloacae | - |
culture condition | L-asparaginase-I is constitutive | Saccharomyces cerevisiae | - |
culture condition | L-asparaginase-II is secreted in response to N starvation | Saccharomyces cerevisiae | - |
culture condition | sabourand dextrose broth yields maximum growth and maximum L-asparaginase production | Nocardia asteroides | - |
flower bud | - |
Lupinus arboreus | - |
leaf | - |
Lupinus arboreus | - |
additional information | a pH of 7.9, casein hydrolysate (3.11%) and corn-steep liquor (3.68%) are the most significant factors improving the enzyme production process | Pseudomonas aeruginosa | - |
additional information | enzyme activity is highest during the exponential phase of growth | Rhodotorula mucilaginosa | - |
additional information | highest L-asparaginase production in 2% proline medium, the lowest L-asparaginase production levels is found in the presence of glutamine and urea as nitrogen sources | Aspergillus tamarii | - |
additional information | production of high levels of L-asparaginase in solid state fermentation, using agro-wastes from three leguminous crops (bran of Cajanus cajan, Phaseolus mungo, and Glycine max) | Aspergillus niger | - |
additional information | the lowest L-asparaginase production levels is found in the presence of glutamine and urea as nitrogen sources | Aspergillus terreus | - |
additional information | the synthesis of L-asparaginase in Escherichia coli W and Escherichia coli K-12 is almost completely suppressed if glucose is added at a concentration of 0.5% to the growth medium. Organic acids and amino acids such as L-leucine and L-methionine enhance production of L-asparaginase. n-Dodecane at 6% increases cell concentration by 12.7% and production of L-asparaginase by 21% to give 60.8 IU/ml in the fermentation medium | Escherichia coli | - |
root tip | - |
Lupinus arboreus | - |
seed | developing | Lupinus arboreus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Mycolicibacterium phlei | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Erwinia sp. | L-aspartate + NH3 | - |
? | |
additional information | L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora | Escherichia coli | ? | - |
? | |
additional information | L-asparaginase from Escherichia coli is more immuno-depressive and immunotoxic than that from Erwinia carotovora | Pectobacterium carotovorum | ? | - |
? | |
additional information | does not hydrolyse L-glutamine | Erwinia sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Thermus thermophilus |
hexamer | - |
Erwinia sp. |
homodimer | - |
Rhodotorula toruloides |
Synonyms | Comment | Organism |
---|---|---|
L-asparaginase-I | - |
Saccharomyces cerevisiae |
L-asparaginase-II | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 32 | - |
Staphylococcus sp. |
37 | - |
- |
Rhodotorula toruloides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
77 | - |
the enzyme follows linear kinetics, even at 77°C | Erwinia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable for 10 min | Cyberlindnera jadinii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Cyberlindnera jadinii |
6.4 | - |
- |
Rhodotorula toruloides |
8.2 | - |
- |
Sphagnum fallax |
8.6 | - |
- |
Thermus thermophilus |
8.6 | 8.8 | - |
Staphylococcus sp. |
9.2 | - |
- |
Erwinia sp. |