Literature summary for 3.5.1.1 extracted from

Bruneau, L.; Chapman, R.; Marsolais, F.
Co-occurrence of both L-asparaginase subtypes in Arabidopsis: At3g16150 encodes a K+-dependent L-asparaginase (2006), Planta, 224, 668-679.

Search for more literature for 3.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli as N-terminal His-tagged protein	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	catalytic activity of At3g16150 is enhanced approximately tenfold in the presence of K+	Arabidopsis thaliana
Na+	monovalent cation preference in order of decreasing efficiency is: K+, Na+, Rb+	Arabidopsis thaliana
Rb+	monovalent cation preference in order of decreasing efficiency is: K+, Na+, Rb+	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14200	-	x * 27000 + x * 14200, SDS-PAGE	Arabidopsis thaliana
27000	-	x * 27000 + x * 14200, SDS-PAGE	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q8GXG1	ecotype Columbia	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower	activity is highest in sink tissues, especially in flowers and siliques	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-asparagine + H2O	strictly specific for L-Asn, has no activity towards beta-aspartyl dipeptides	Arabidopsis thaliana	L-aspartate + NH3	-	?
L-aspartyl hydroxamate + H2O	-	Arabidopsis thaliana	L-aspartate + hydroxylamine	-	?
additional information	not active with N-acetylglucosaminyl-L-Asn	Arabidopsis thaliana	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 27000 + x * 14200, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
At3g16150	-	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)