Literature summary for 3.5.1.1 extracted from

Bansal, S.; Gnaneswari, D.; Mishra, P.; Kundu, B.
Structural stability and functional analysis of L-asparaginase from Pyrococcus furiosus (2010), Biochemistry (Moscow), 75, 375-381.

Cloned(Commentary)

Cloned (Comment)	Organism
cloned and expressed in Escherichia coli	Pyrococcus furiosus

General Stability

General Stability	Organism
in the presence of guanidine hydrochloride (GdnCl) the curves follow a sigmoidal pattern with unfolding beginning at 3.25 M guanidine hydrochloride and complete unfolding achieved at 4.75 M guanidine hydrochloride. The enzymatic activity of urea-containing protein samples remains unchanged. In contrast, the guanidine hydrochloride-incubated samples show a significantly lower activity even with 1 M guanidine hydrochloride.	Pyrococcus furiosus

Organism

in the presence of guanidine hydrochloride (GdnCl) the curves follow a sigmoidal pattern with unfolding beginning at 3.25 M guanidine hydrochloride and complete unfolding achieved at 4.75 M guanidine hydrochloride. The enzymatic activity of urea-containing protein samples remains unchanged. In contrast, the guanidine hydrochloride-incubated samples show a significantly lower activity even with 1 M guanidine hydrochloride.

Pyrococcus furiosus

Inhibitors

Inhibitors	Comment	Organism	Structure
L-asparagine	substrate inhibition above 20 mM L-asparagine	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	2 * 37000, SDS-PAGE, 2 * 37860, mass spectrometry	Pyrococcus furiosus
37860	-	2 * 37000, SDS-PAGE, 2 * 37860, mass spectrometry	Pyrococcus furiosus
75450	-	gel filtration, Superdex-200 column, single peak	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity	Pyrococcus furiosus

Subunits

Subunits	Comment	Organism
dimer	2 * 37000, SDS-PAGE, 2 * 37860, mass spectrometry	Pyrococcus furiosus

Synonyms

Synonyms	Comment	Organism
L-asparaginase	-	Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	-	assay at	Pyrococcus furiosus
85	-	about 85°C, activity increases progressively with increase in temperature up to and beyond 85°C	Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
55	85	enzyme incubation for different time periods monitored over a range of 55-85°C shows linear kinetics. From the plot the activation energy is found to be 42.5 kJ/mol*K	Pyrococcus furiosus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Pyrococcus furiosus

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	10.5	an increase in enzyme activity is observed with increase in pH up to a maximum at pH 9 followed by a sharp fall	Pyrococcus furiosus