Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.1 extracted from

  • El-Bessoumy, A.A.; Sarhan, M.; Mansour, J.
    Production, isolation, and purification of L-asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation (2004), J. Biochem. Mol. Biol., 37, 387-393.
    View publication on PubMed

Application

Application Comment Organism
medicine used for treatment of acute lymphoblastic leukemia, pancreatic carcinoma, and bovine lymphosarcoma Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.147
-
L-Asn
-
Pseudomonas aeruginosa
0.147
-
L-asparagine pH 9.0, 37°C Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
gel filtration Pseudomonas aeruginosa
160000
-
SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Asn + H2O Pseudomonas aeruginosa
-
L-Asp + NH3
-
?
L-Asn + H2O Pseudomonas aeruginosa 50071
-
L-Asp + NH3
-
?
L-asparagine + H2O Pseudomonas aeruginosa
-
L-aspartate + NH3
-
?
L-asparagine + H2O Pseudomonas aeruginosa 50071
-
L-aspartate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa
-
solid state fermentation
-
Pseudomonas aeruginosa 50071
-
-
-
Pseudomonas aeruginosa 50071
-
solid state fermentation
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa
native enzyme from strain 50071, grown on solid-state fermentation, 106fold to homogeneity by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1900
-
purified enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asn + H2O
-
Pseudomonas aeruginosa L-Asp + NH3
-
?
L-Asn + H2O
-
Pseudomonas aeruginosa 50071 L-Asp + NH3
-
?
L-asparagine + H2O
-
Pseudomonas aeruginosa L-aspartate + NH3
-
?
L-asparagine + H2O
-
Pseudomonas aeruginosa 50071 L-aspartate + NH3
-
?

Subunits

Subunits Comment Organism
monomer 1 * 160000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
L-asparaginase
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
Pseudomonas aeruginosa
37
-
-
Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60
-
Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
4 11
-
Pseudomonas aeruginosa