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0.018
-
TfCel9A wild-type, activity on 600 microM G5
0.03
substrate carboxymethylcellulose, pH 5.0, 50°C
0.037
-
TfCel9A W256A mutant, activity on 600 microM G5
0.04
-
substrate: carboxymethylcellulose, pH 5.0, 50°C
0.048
-
partially purified enzyme, pH 4.5, 55°C
0.049
Sporotrichum pulverulentum
-
partially purified enzyme, pH 4.5, 55°C
0.075
-
substrate: carboxymethylcellulose, pH 5.0, 50°C
0.1
-
substrate: carboxymethylcellulose, pH 5.0, 70°C
0.13
-
substrate: carboxymethylcellulose, pH 5.0, 70°C
0.16
substrate phosphoric acid swollen cellulose, pH 6.0, 60°C
0.166
-
after DEAE anion exchange chromatography
0.175
-
substrate: carboxymethylcellulose, pH 5.0, 50°C
0.25
wild-type, pH 7.0, 60°C
0.39
mutant S365P, pH 7.0, 40°C
0.44
mutant K94R, pH 7.0, 60°C
0.54
substrate filter paper , pH 6.0, 60°C
0.56
-
cells in bioreactor
0.673
-
foregut proventriculus
0.68
Thermochaetoides thermophila
substrate carboxymethylcellulose, mutant Y30F/Y173F, pH 4.0, 60°C
0.7
-
wild-type, pH 4.8, 50°C
0.89
-
substrate filter paper, pH 5.5, 60°C
1.02
substrate beta-glucan, pH 6.0, 60°C
1.2
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 1.8, 80°C
1.3
-
purified enzyme MtGH45, substrate phosphoric acid swollen cellulose, pH 6.5, 50°C
1.35
Thermochaetoides thermophila
substrate carboxymethylcellulose, mutant Y30F, pH 4.0, 60°C
1.52
Thermochaetoides thermophila
substrate carboxymethylcellulose, pH 4.0, 70°C
1.67
Thermochaetoides thermophila
substrate beta-D-glucan, mutant Y173F, pH 4.0, 60°C
1.87
Thermochaetoides thermophila
substrate carboxymethylcellulose, mutant Y173F, pH 4.0, 60°C
1.91
-
Cel5A, carboxymethyl cellulose as substrate
10
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
10.9
purified recombinant enzyme, pH 5.0, 60°C, substrate Avicel PH-101
101
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
103
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
105.8
-
presence of 0.5% Tween-20, pH 6.0, 65°C
108
substrate hydroxyethyl cellulose, pH 5.5, 92°C
109
-
purified recombinant mutant K429A
11.02
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from cutter-milled rice straw as carbon source
11.61
-
after DEAE anion exchange chromatography
117
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week, Exophiala sp. aff. salmonis, GenBank Accession: HQ630990
12.8
-
purified enzyme MtGH45, substrate cotton, pH 6.5, 50°C
120
-
pH 5.5, 80°C, substrate carboxymethyl cellulose
123
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
125
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
127.2
-
presence of 96% glycerol, pH 4.8, 4°C
13.9
substrate barley beta-glucan, pH 3.5, 40°C
130
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Phoma sp. aff. glomerata, GenBank Accession: HQ630999
134
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
134.22
purified recombinant enzyme, pH 5.0, 60°C, substrate lichenin
137
-
purified recombinant mutant G91A
138
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
1443
substrate beta-glucan, pH 5.0, 50°C
15
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Phoma sp. aff. glomerata, GenBank Accession: HQ630999
150
-
activity with carboxymethylcellulose
154
-
purified recombinant mutant Y97W
156
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
16
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
16.5
-
catalytic domain of endoglucanase G
16.76
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from Solka Floc as carbon source
162
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
17.3
-
avicelase activity, pH 5.5, 50°C
170.7
-
purified enzyme GtGH45, substrate beta-glucan, pH 6.5, 50°C
174
substrate carboxymethyl cellulose, pH 5.0, 50°C
18.63
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from dry ball-milled rice straw as carbon source
186
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
19.73
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from freeze-dried rice straw after wet disk milling at 7 days as carbon source
192
-
substrate medium viscosity carboxymethyl cellulose, pH 5.5, 60°C
193
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
1970
substrate carboxymethyl cellulose, pH 5.5, 30°C
2.14
Thermochaetoides thermophila
substrate beta-1,4-D-glucan, pH 4.0, 70°C
2.22
Thermochaetoides thermophila
purified recombinant His-tagged enzyme, pH 5.0, 55°C, substrate beta-glucan
2.8
debranched arabinan, relative activity 51%
2.9
arabinan, relative activity 53%
20
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
20.5
substrate lichenan, pH 3.5, 40°C
200.6
-
purified enzyme GtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C
202
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
202.6
-
purified enzyme MtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C
2020
substrate barley beta-glucan, pH 5.0, 60°C
208
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
209.9
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate lichenan
21.8
substrate konjac mannan, pH 6.5, 50°C
210.7
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate barley beta-glucan
218.9
-
substrate cellobiose, crude enzyme preparation, pH 8.0, 60°C
220
Rucel5B, pH 6.5, 60°C, substrate carboxymethyl cellulose
229
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
23
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
234
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
234.6
-
substrate cotton straw, crude enzyme preparation, pH 8.0, 60°C
24.1
-
isoform endoglucanase 2, pH 5.0, 50°C
246
-
substrate low viscosity carboxymethyl cellulose, pH 5.5, 60°C
248.7
recombinant CSCMCase
25
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
2500
-
pH not specified in the publication, temperature not specified in the publication
251
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
2549
-
purified native enzyme
256
substrate barley beta-glucan, pH 6.5, 50°C
257.8
-
substrate wheat straw, crude enzyme preparation, pH 8.0, 60°C
26
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
264.7
presence of 96% glycerol, pH 4.8, 4°C
269
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
27
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
27.3
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 6.0, 40°C
272
substrate lichenan, pH 5.5, 92°C
28.1
cellulase activity is estimated by the AZO-carboxymethylcellulose standard assay, last purification step
280
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
29
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
297.15
purified recombinant His-tagged enzyme, pH 5.0, 55°C, substrate carboxymethyl cellulose
3.88
-
M44-11 mutant, carboxymethyl cellulose as substrate
315
-
recombinant enzyme from cytoplasmic supernatant of Escherichia coli
315.2
-
substrate 4-nitrophenyl beta-D-glucopyranoside, crude enzyme preparation, pH 8.0, 60°C
317
substrate barley beta-glucan, pH 5.5, 92°C
32.4
-
purified native enzyme
34.4
wild-type, pH 7.0, 50°C
3413
substrate lichenan, pH 4.5, 90°C
349
-
purified recombinant mutant G91A/Y97W
35.6
-
purified enzyme MtGH45, substrate lichenan, pH 6.5, 50°C
35.8
substrate carboxymethylcellulose, pH 3.5, 40°C
354.1
-
substrate rice straw, crude enzyme preparation, pH 8.0, 60°C
357
-
purified recombinant mutant Y97W/K429A
372
substrate barley beta-glucan, pH 6.5, 70°C
38.5
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate carboxymethyl cellulose
38.7
Thermochaetoides thermophila
-
pH 5.0, 60°C
384
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
39.2
-
carboxymethylcellulase activity, pH 5.5, 50°C
4
birchwood xylan, relative activity 73%
4.2
oat spelt xylan, relative activity 76%
4.6
beechwood xylan, relative activity 84%
4.84
-
S75 mutant, carboxymethyl cellulose as substrate
4.88
-
S78 mutant, carboxymethyl cellulose as substrate
4.93
carboxymethyl cellulose sodium salt
40
-
enzyme mutant F194A, pH 5.5, 50°C
4040
substrate Hordeum vulgare beta-glucan
4059
substrate laminarin, pH 4.5, 90°C
42.3
-
substrate carboxymethyl cellulose, pH 5.5, 40°C
4240
-
purified type I CMCase, pH 4.8, 40°C
4254
substrate barley beta-glucan, pH 4.5, 90°C
429.7
-
substrate corn stover, crude enzyme preparation, pH 8.0, 60°C
43.02
-
purified native enzyme, pH 6.5, 60°C
469
-
purified recombinant mutant G91A/Y97W/K429A
47
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
47.6
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 4.5, 40°C
5.13
-
after DEAE anion exchange chromatography
5.5
carboxymethylcellulose, relative activity 100%
5.55
-
mutant E289V, pH 4.8, 55°C
51
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
510.8
-
substrate carboxymethyl cellulose, crude enzyme preparation, pH 8.0, 60°C
52
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
52.2
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 6.0, 40°C
52.5
-
purified enzyme MtGH45, substrate beta-glucan, pH 6.5, 50°C
54.2
-
purified enzyme GtGH45, substrate lichenan, pH 6.5, 50°C
57.92
-
purified native enzyme, pH 5.0, 50°C
59.1
-
carboxymethyl cellulase supernatant, xylose as a substrate
6.4
pH 7.0, 40°C, presence of 2 M NaCl
60
-
wild-type, substrate barley beta-glucan, pH 5.0, 40°C
61
purified recombinant mutant S127C/A165C, substrate beta-glucan, pH and temperature not specified in the publication
624
substrate carboxymethyl cellulose, pH 4.5, 90°C
64.17
-
purified native enzyme
64.8
-
catalytic domain expressed in Escherichia coli
65.53
purified recombinant enzyme, pH 5.0, 60°C, substrate carboxymethyl cellulose
66
purified recombinant mutant Y171C/L201C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication
66.1
-
endo-beta-1,4-glucanase E1
66.4
-
endo-beta-1,4-glucanase E2 and E3
67
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
692
substrate carboxymethyl cellulose, pH 5.5, 92°C
7.43
-
after gel filtration chromatography
74
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
76
substrate carboxymethyl cellulose, pH 5.0, 50°C
77
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
78
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
8.13
purified recombinant enzyme, pH 5.0, 60°C, substrate laminarin
8.5
-
purified enzyme GtGH45, substrate cotton, pH 6.5, 50°C
81
-
mutant N194A, substrate barley beta-glucan, pH 5.0, 40°C
82
substrate carboxymethyl cellulose, pH 6.0, 40°C
83
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
84
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
846.8
-
purified type II CMCase, pH 4.8, 40°C
87
purified recombinant His-tagged enzyme, substrate phosphoric acid swollen cellulose, pH 5.0, 50°C
9
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
9.06
-
isoform endoglucanase 1, pH 5.0, 50°C
92.29
purified recombinant enzyme, pH 5.0, 60°C, substrate barley beta-glucan
92.5
-
gel filtration chromatography, last purification step
94
substrate lichenan, pH 5.0, 50°C
96
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
97.95
-
purified native enzyme, pH 5.0, 70°C
0.05
-
partially purified enzyme, pH 4.5, 55°C
0.05
-
substrate: carboxymethylcellulose, pH 5.0, 50°C
0.14
substrate barley beta-glucan, pH 5.0, 50°C
0.14
-
substrate: carboxymethylcellulose, pH 5.0, 70°C
0.19
substrate lichenan, pH 5.0, 50°C
0.19
-
substrate: carboxymethylcellulose, pH 5.0, 70°C
0.21
substrate carboxymethyl cellulose, pH 6.0, 60°C
0.21
-
substrate: carboxymethylcellulose, pH 5.0, 70°C
0.33
mutant S365P, pH 7.0, 60°C
0.33
wild-type, pH 7.0, 40°C
0.45
mutant K94R, pH 7.0, 40°C
0.45
Thermochaetoides thermophila
substrate beta-D-glucan, mutant Y30F/Y173F, pH 4.0, 60°C
0.8
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
0.8
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH and temperature not specified in the publication, hybrid protein (CelA-SSO1949-CelA, consisting of 262 amino acids) based on beta-endoglucanase from Sulfolobus solfataricus P2 and beta-endoglucanase from Thermotoga maritima
0.8
substrate N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
1
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
1
Thermochaetoides thermophila
substrate beta-D-glucan, wild-type, pH 4.0, 60°C
1.05
CM cellulose
1.05
substrate lichenan, pH 6.0, 60°C
1.21
Thermochaetoides thermophila
substrate beta-D-glucan, mutant Y30F, pH 4.0, 60°C
1.21
Thermochaetoides thermophila
substrate carboxymethylcellulose, wild-type, pH 4.0, 60°C
11
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
11
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
115
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
115
substrate xyloglucan, pH 6.5, 50°C
12.3
-
carboxymethyl cellulase supernatant, glucose as a substrate
12.3
substrate birchwood xylan, pH 4.5, 90°C
12.74
-
last purification step
12.74
-
after gel filtration chromatography
13
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
13
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
14
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
14
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
1444
substrate barley beta-glucan, pH 5.0, 50°C
1444
substrate beta-glucan, pH 5.0, 50°C
159
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
159
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks, Exophiala sp. aff. salmonis, GenBank Accession: HQ630990
159
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
17
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
17
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
176
substrate carob bean gum, pH 4.5, 90°C
176
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
18
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
18
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
18
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
19
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
19
-
recombinant protein, pH 7, 60°C
19.09
-
last purification step
19.09
-
after gel filtration chromatography
2
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
2
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
21
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
21
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
22
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
22
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
22
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
24
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
24
purified recombinant His-tagged enzyme, substrate lichenan, pH 5.0, 50°C
24
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
24
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
24
purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.0, 30°C
248
-
substrate barley beta-glucan, pH 5.5, 60°C
248
-
purified recombinant mutant G91A/K429A
3
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
3
-
purified enzyme MtGH45, substrate Avicel, pH 6.5, 50°C
3.3
substrate carboxymethylcellulose, pH 6.5, 50°C
3.3
-
purified enzyme GtGH45, substrate phosphoric acid swollen cellulose, pH 6.5, 50°C
3.3
Thermochaetoides thermophila
-
pH 5.0, 50°C
31
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
31
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week, Phoma sp. aff. glomerata, GenBank Accession: HQ630999
32
purified recombinant His-tagged enzyme, substrate beta-glucan, pH 5.0, 50°C
32
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
33
presence of 2.5 M NaCl, pH 5.0, 55°C
33
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
34
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
34
substrate phosphoric acid-swollen cellulose, pH 5.5, 92°C
35
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
35
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
39
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
39
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
42
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Exophiala sp. aff. spinifera, GenBank Accession: HQ631027
42
-
mutant N19A, substrate carboxymethylcellulose, pH 5.0, 40°C
428
-
recombinant enzyme from granules produced by Escherichia coli
428
purified recombinant His-tagged enzyme, pH 4.0, 95°C, substrate carboxymethyl cellulose
44
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
44
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
450
-
after ion-exchange chromatography
46
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
46
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks, Phoma sp. aff. glomerata, GenBank Accession: HQ630999
48
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
48
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
53
-
mutant N19A, substrate barley beta-glucan, pH 5.0, 40°C
53
purified recombinant wild-type enzyme, substrate beta-glucan, pH and temperature not specified in the publication
53
substrate carboxymethyl cellulose, pH 6.0, 40°C
57
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
57
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
57
-
wild-type, substrate carboxymethylcellulose, pH 5.0, 40°C
57
purified recombinant wild-type enzyme, substrate carboxymethyl cellulose, pH and temperature not specified in the publication
58
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
58
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Exophiala sp. aff. salmonis, GenBank Accession: HQ630990
622
substrate glucomannan, pH 5.0, 50°C
622
substrate glucomannan, pH 5.0, 50°C
64
purified recombinant His-tagged enzyme, substrate glucomannan, pH 5.0, 50°C
64
-
mutant N42A, substrate carboxymethylcellulose, pH 5.0, 40°C
64
purified recombinant mutant Y171C/L201C, substrate beta-glucan, pH and temperature not specified in the publication
65
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
65
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Phoma sp. aff. glomerata, GenBank Accession: HQ630999
68
-
mutant N42A, substrate barley beta-glucan, pH 5.0, 40°C
68
purified recombinant mutant S127C/A165C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication
68
substrate carboxymethyl cellulose, pH 4.5, 40°C
694
substrate carboxymethyl cellulose, pH 5.0, 50°C
694
substrate carboxymethyl cellulose, pH 5.0, 50°C
75
substrate beta-glucan, pH 5.0, 50°C
75
-
mutant N194A, substrate carboxymethylcellulose, pH 5.0, 40°C
79
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
79
purified recombinant His-tagged enzyme, substrate carboxymethyl cellulose, pH 5.0, 50°C
8.8
pH 5.5, 37°C
8.8
-
purified enzyme GtGH45, substrate Avicel, pH 6.5, 50°C
80
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Exophiala sp. aff. salmonis, GenBank Accession: HQ630990
80
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
additional information
-
-
additional information
-
-
additional information
-
purified native enzyme, 1289.83 U/ml, pH 5.0, 50°C
additional information
-
-
additional information
2.82 U/ml for the native extracellular enzyme, 6.78-8.54 U/ml for the recombinant extracellular enzyme expressed in Escherichia coli strain BL21(DE3)
additional information
-
2.82 U/ml for the native extracellular enzyme, 6.78-8.54 U/ml for the recombinant extracellular enzyme expressed in Escherichia coli strain BL21(DE3)
additional information
-
-
additional information
recombinant crude enzyme, 5.772 U/ml
additional information
-
recombinant crude enzyme, 5.772 U/ml
additional information
-
-
additional information
Lenzites trabea
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
-
-
additional information
pH 5.0, specific activity of the enzyme recombinantly expressed in Sulfolobus solfataricus is about 200fold higher compared to the specific activity of the enzyme recombinantly expressed in Escherichia coli
additional information
-
pH 5.0, specific activity of the enzyme recombinantly expressed in Sulfolobus solfataricus is about 200fold higher compared to the specific activity of the enzyme recombinantly expressed in Escherichia coli
additional information
Sporotrichum pulverulentum
-
discontinuous test based on the specific determination of the cellobiose produced during the cellulase reaction with an ancillary cellobiose dehydrogenase
additional information
substrate carboxymethyl cellulose, recombinant wild-type enzyme shows 70.4 mU/ml activity, activities of enzyme mutants G263C/R307H, G145D/N207K, P228R, T67N/D142E/S218N/V242D/D330E, and T157I/G251D/V259D are 103.8, 88.6, 112.6, 89.1, and 102.9 mU/ml, respectively
additional information
-
discontinuous test based on the specific determination of the cellobiose produced during the cellulase reaction with an ancillary cellobiose dehydrogenase
additional information
-
-
additional information
-
-
additional information
-
discontinuous test based on the specific determination of the cellobiose produced during the cellulase reaction with an ancillary cellobiose dehydrogenase
additional information
-
assay methods
additional information
-
-
additional information
-
assay methods
additional information
-
staining techniques for the detection
additional information
-
assay methods
additional information
-
discontinuous test based on the specific determination of the cellobiose produced during the cellulase reaction with an ancillary cellobiose dehydrogenase