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3.2.1.4: cellulase

This is an abbreviated version!
For detailed information about cellulase, go to the full flat file.

Word Map on EC 3.2.1.4

Reaction

cellohexaose
+
H2O
= 2 cellotriose

Synonyms

(1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.4 cellulase

Specific Activity

Specific Activity on EC 3.2.1.4 - cellulase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.018
-
TfCel9A wild-type, activity on 600 microM G5
0.023
-
homogenate
0.03
substrate carboxymethylcellulose, pH 5.0, 50°C
0.037
-
TfCel9A W256A mutant, activity on 600 microM G5
0.04
-
substrate: carboxymethylcellulose, pH 5.0, 50°C
0.048
-
partially purified enzyme, pH 4.5, 55°C
0.049
Sporotrichum pulverulentum
-
partially purified enzyme, pH 4.5, 55°C
0.075
-
substrate: carboxymethylcellulose, pH 5.0, 50°C
0.1
-
substrate: carboxymethylcellulose, pH 5.0, 70°C
0.13
-
substrate: carboxymethylcellulose, pH 5.0, 70°C
0.16
substrate phosphoric acid swollen cellulose, pH 6.0, 60°C
0.166
-
after DEAE anion exchange chromatography
0.175
-
substrate: carboxymethylcellulose, pH 5.0, 50°C
0.23
-
homogenate
0.25
wild-type, pH 7.0, 60°C
0.29
-
cells in flasks
0.367
-
midgut
0.39
mutant S365P, pH 7.0, 40°C
0.44
mutant K94R, pH 7.0, 60°C
0.54
substrate filter paper , pH 6.0, 60°C
0.56
-
cells in bioreactor
0.669
-
hindgut
0.673
-
foregut proventriculus
0.68
Thermochaetoides thermophila
substrate carboxymethylcellulose, mutant Y30F/Y173F, pH 4.0, 60°C
0.7
-
wild-type, pH 4.8, 50°C
0.71
-
foregut crop
0.89
-
substrate filter paper, pH 5.5, 60°C
1.02
substrate beta-glucan, pH 6.0, 60°C
1.2
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 1.8, 80°C
1.254
-
salivary gland
1.3
-
purified enzyme MtGH45, substrate phosphoric acid swollen cellulose, pH 6.5, 50°C
1.35
Thermochaetoides thermophila
substrate carboxymethylcellulose, mutant Y30F, pH 4.0, 60°C
1.52
Thermochaetoides thermophila
substrate carboxymethylcellulose, pH 4.0, 70°C
1.67
Thermochaetoides thermophila
substrate beta-D-glucan, mutant Y173F, pH 4.0, 60°C
1.87
Thermochaetoides thermophila
substrate carboxymethylcellulose, mutant Y173F, pH 4.0, 60°C
1.91
-
Cel5A, carboxymethyl cellulose as substrate
10
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
10.9
purified recombinant enzyme, pH 5.0, 60°C, substrate Avicel PH-101
101
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
103
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
105.8
-
presence of 0.5% Tween-20, pH 6.0, 65°C
108
substrate hydroxyethyl cellulose, pH 5.5, 92°C
109
-
purified recombinant mutant K429A
11.02
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from cutter-milled rice straw as carbon source
11.61
-
after DEAE anion exchange chromatography
117
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week, Exophiala sp. aff. salmonis, GenBank Accession: HQ630990
12.74
12.8
-
purified enzyme MtGH45, substrate cotton, pH 6.5, 50°C
120
-
pH 5.5, 80°C, substrate carboxymethyl cellulose
123
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
123600
-
pH 7.0, 65°C
125
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
127.2
-
presence of 96% glycerol, pH 4.8, 4°C
13.06
-
-
13.9
substrate barley beta-glucan, pH 3.5, 40°C
130
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Phoma sp. aff. glomerata, GenBank Accession: HQ630999
134
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
134.22
purified recombinant enzyme, pH 5.0, 60°C, substrate lichenin
137
-
purified recombinant mutant G91A
138
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
139.6
-
pH 4.8, 4°C
14.4
-
pH 4.0, 60°C
1443
substrate beta-glucan, pH 5.0, 50°C
15
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Phoma sp. aff. glomerata, GenBank Accession: HQ630999
150
-
activity with carboxymethylcellulose
154
-
purified recombinant mutant Y97W
156
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
16
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
16.5
-
catalytic domain of endoglucanase G
16.76
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from Solka Floc as carbon source
16.88
-
pH 6.0, 65°C
162
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
17.3
-
avicelase activity, pH 5.5, 50°C
170.7
-
purified enzyme GtGH45, substrate beta-glucan, pH 6.5, 50°C
174
substrate carboxymethyl cellulose, pH 5.0, 50°C
18.63
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from dry ball-milled rice straw as carbon source
186
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
19.09
19.73
-
carboxymethyl cellulase, pH 4.8, 45°C, culture from freeze-dried rice straw after wet disk milling at 7 days as carbon source
192
-
substrate medium viscosity carboxymethyl cellulose, pH 5.5, 60°C
193
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
1970
substrate carboxymethyl cellulose, pH 5.5, 30°C
2.14
Thermochaetoides thermophila
substrate beta-1,4-D-glucan, pH 4.0, 70°C
2.22
Thermochaetoides thermophila
purified recombinant His-tagged enzyme, pH 5.0, 55°C, substrate beta-glucan
2.8
debranched arabinan, relative activity 51%
2.9
arabinan, relative activity 53%
20
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
20.5
substrate lichenan, pH 3.5, 40°C
200.6
-
purified enzyme GtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C
202
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
202.6
-
purified enzyme MtGH45, substrate carboxymethyl cellulose, pH 6.5, 50°C
2020
substrate barley beta-glucan, pH 5.0, 60°C
208
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
209.9
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate lichenan
21.8
substrate konjac mannan, pH 6.5, 50°C
210.7
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate barley beta-glucan
218.9
-
substrate cellobiose, crude enzyme preparation, pH 8.0, 60°C
220
Rucel5B, pH 6.5, 60°C, substrate carboxymethyl cellulose
229
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
23
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
234
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
234.6
-
substrate cotton straw, crude enzyme preparation, pH 8.0, 60°C
24.1
-
isoform endoglucanase 2, pH 5.0, 50°C
246
-
substrate low viscosity carboxymethyl cellulose, pH 5.5, 60°C
248.7
recombinant CSCMCase
25
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
2500
-
pH not specified in the publication, temperature not specified in the publication
251
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
2549
-
purified native enzyme
256
substrate barley beta-glucan, pH 6.5, 50°C
257.8
-
substrate wheat straw, crude enzyme preparation, pH 8.0, 60°C
26
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
26.8
pH 5.0, 50°C
264.7
presence of 96% glycerol, pH 4.8, 4°C
269
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
27
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
27.3
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 6.0, 40°C
272
substrate lichenan, pH 5.5, 92°C
2740
substrate lichenan
28.1
cellulase activity is estimated by the AZO-carboxymethylcellulose standard assay, last purification step
280
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
29
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
297.15
purified recombinant His-tagged enzyme, pH 5.0, 55°C, substrate carboxymethyl cellulose
3.77
-
pH 7.0, 70°C
3.88
-
M44-11 mutant, carboxymethyl cellulose as substrate
315
-
recombinant enzyme from cytoplasmic supernatant of Escherichia coli
315.2
-
substrate 4-nitrophenyl beta-D-glucopyranoside, crude enzyme preparation, pH 8.0, 60°C
317
substrate barley beta-glucan, pH 5.5, 92°C
32.4
-
purified native enzyme
34.4
wild-type, pH 7.0, 50°C
3413
substrate lichenan, pH 4.5, 90°C
349
-
purified recombinant mutant G91A/Y97W
35.6
-
purified enzyme MtGH45, substrate lichenan, pH 6.5, 50°C
35.8
substrate carboxymethylcellulose, pH 3.5, 40°C
354.1
-
substrate rice straw, crude enzyme preparation, pH 8.0, 60°C
357
-
purified recombinant mutant Y97W/K429A
372
substrate barley beta-glucan, pH 6.5, 70°C
38.5
purified recombinant His-tagged enzyme, pH 6.0, 115°C, substrate carboxymethyl cellulose
38.7
Thermochaetoides thermophila
-
pH 5.0, 60°C
384
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
39.2
-
carboxymethylcellulase activity, pH 5.5, 50°C
4
birchwood xylan, relative activity 73%
4.2
oat spelt xylan, relative activity 76%
4.6
beechwood xylan, relative activity 84%
4.84
-
S75 mutant, carboxymethyl cellulose as substrate
4.88
-
S78 mutant, carboxymethyl cellulose as substrate
4.93
carboxymethyl cellulose sodium salt
40
-
enzyme mutant F194A, pH 5.5, 50°C
4040
substrate Hordeum vulgare beta-glucan
4059
substrate laminarin, pH 4.5, 90°C
42.3
-
substrate carboxymethyl cellulose, pH 5.5, 40°C
4240
-
purified type I CMCase, pH 4.8, 40°C
4254
substrate barley beta-glucan, pH 4.5, 90°C
429.7
-
substrate corn stover, crude enzyme preparation, pH 8.0, 60°C
43.02
-
purified native enzyme, pH 6.5, 60°C
43.5
pH 3.5, 70°C
44.9
-
pH 4.6, 60°C
469
-
purified recombinant mutant G91A/Y97W/K429A
47
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
47.6
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 4.5, 40°C
5.13
-
after DEAE anion exchange chromatography
5.5
carboxymethylcellulose, relative activity 100%
5.55
-
mutant E289V, pH 4.8, 55°C
51
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 4 weeks
51.3
-
pH 4.0, 55°C
510.8
-
substrate carboxymethyl cellulose, crude enzyme preparation, pH 8.0, 60°C
52
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
52.2
substrate 4''',6'''-O-benzylidene 2-chloro-4-nitrophenyl-beta-cellotrioside, pH 6.0, 40°C
52.5
-
purified enzyme MtGH45, substrate beta-glucan, pH 6.5, 50°C
54.2
-
purified enzyme GtGH45, substrate lichenan, pH 6.5, 50°C
57.92
-
purified native enzyme, pH 5.0, 50°C
59.1
-
carboxymethyl cellulase supernatant, xylose as a substrate
6.4
pH 7.0, 40°C, presence of 2 M NaCl
6.52
-
pH 4.5, 30°C
60
-
wild-type, substrate barley beta-glucan, pH 5.0, 40°C
61
purified recombinant mutant S127C/A165C, substrate beta-glucan, pH and temperature not specified in the publication
624
substrate carboxymethyl cellulose, pH 4.5, 90°C
64.17
-
purified native enzyme
64.8
-
catalytic domain expressed in Escherichia coli
65.1
-
endoglucanase I
65.53
purified recombinant enzyme, pH 5.0, 60°C, substrate carboxymethyl cellulose
66
purified recombinant mutant Y171C/L201C, substrate carboxymethyl cellulose, pH and temperature not specified in the publication
66.1
-
endo-beta-1,4-glucanase E1
66.4
-
endo-beta-1,4-glucanase E2 and E3
67
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks
692
substrate carboxymethyl cellulose, pH 5.5, 92°C
7.43
-
after gel filtration chromatography
71.2
-
pH 9.0, 50°C
74
-
37°C, pH 5.0, release of D-glucose from carboxymethyl cellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 1 week
76
substrate carboxymethyl cellulose, pH 5.0, 50°C
77
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
78
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Phoma sp. aff. herbarum, GenBank Accession: HQ630963
787
-
pH 5.0, 35°C
8.13
purified recombinant enzyme, pH 5.0, 60°C, substrate laminarin
8.5
-
purified enzyme GtGH45, substrate cotton, pH 6.5, 50°C
81
-
mutant N194A, substrate barley beta-glucan, pH 5.0, 40°C
82
substrate carboxymethyl cellulose, pH 6.0, 40°C
83
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
84
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 2 weeks, Arthrinium sp. aff. phaeospermum, GenBank Accession: HQ630967
846.8
-
purified type II CMCase, pH 4.8, 40°C
87
purified recombinant His-tagged enzyme, substrate phosphoric acid swollen cellulose, pH 5.0, 50°C
9
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
9.06
-
isoform endoglucanase 1, pH 5.0, 50°C
92.29
purified recombinant enzyme, pH 5.0, 60°C, substrate barley beta-glucan
92.5
-
gel filtration chromatography, last purification step
928
-
pH 6.0, 50°C
94
substrate lichenan, pH 5.0, 50°C
96
-
37°C, pH 5.0, release of D-glucose from carboxymethylcellulose, activity in crude-cell-free fungal extract collected from fungal cultures on Miscanthus cell walls after 8 weeks
97.95
-
purified native enzyme, pH 5.0, 70°C
additional information