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Literature summary for 3.2.1.4 extracted from
- Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor (2008), J. Exp. Biol., 211, 2275-2287.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
SDS-PAGE | Cherax destructor |
| 52000 | - |
- |
Gecarcoidea natalis |
| 52000 | - |
SDS-PAGE | Gecarcoidea natalis |
| 52000 | - |
endo-beta-1,4-glucanase 2 | Cherax destructor |
| 53000 | - |
SDS-PAGE | Cherax destructor |
| 53000 | - |
endo-beta-1,4-glucanase 1 | Cherax destructor |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cherax destructor | - |
- |
- |
| Cherax destructor Clark 1936 | - |
- |
- |
| Gecarcoidea natalis | - |
- |
- |
| Gecarcoidea natalis Pocock 1888 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration | Cherax destructor |
| gel filtration | Gecarcoidea natalis |
| precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C | Cherax destructor |
| precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C | Gecarcoidea natalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| midgut | - |
Cherax destructor | - |
| midgut | - |
Gecarcoidea natalis | - |
| midgut gland | - |
Cherax destructor | - |
| midgut gland | - |
Gecarcoidea natalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.023 | - |
homogenate | Gecarcoidea natalis |
| 0.166 | - |
after DEAE anion exchange chromatography | Gecarcoidea natalis |
| 0.23 | - |
homogenate | Cherax destructor |
| 5.13 | - |
after DEAE anion exchange chromatography | Cherax destructor |
| 7.43 | - |
after gel filtration chromatography | Cherax destructor |
| 11.61 | - |
after DEAE anion exchange chromatography | Cherax destructor |
| 12.74 | - |
last purification step | Gecarcoidea natalis |
| 12.74 | - |
after gel filtration chromatography | Gecarcoidea natalis |
| 19.09 | - |
last purification step | Cherax destructor |
| 19.09 | - |
after gel filtration chromatography | Cherax destructor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Cherax destructor | D-glucose + cellobiose + cellooligosaccharide | - |
? |  |
| carboxymethyl cellulose + H2O | - |
Cherax destructor Clark 1936 | D-glucose + cellobiose + cellooligosaccharide | - |
? | |
| carboxymethyl cellulose + H2O | - |
Gecarcoidea natalis | glucose + cellobiose + short oligomers | - |
? | |
| carboxymethyl cellulose + H2O | - |
Gecarcoidea natalis Pocock 1888 | glucose + cellobiose + short oligomers | - |
? | |
| carboxymethyl cellulose + H2O | - |
Cherax destructor | additional information | short glucose oligomers, cellobiose and a very small amount of glucose | ? | |
| carboxymethyl cellulose + H2O | - |
Cherax destructor Clark 1936 | additional information | short glucose oligomers, cellobiose and a very small amount of glucose | ? | |
| carboxymethylcellulose + H2O | - |
Gecarcoidea natalis | short glucose oligomers + cellobiose + glucose | - |
? | |
| carboxymethylcellulose + H2O | - |
Gecarcoidea natalis Pocock 1888 | short glucose oligomers + cellobiose + glucose | - |
? | |
| additional information | glucanase hydrolyses beta-1,4-glycosidic bonds | Cherax destructor | ? | - |
? | |
| additional information | glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds | Gecarcoidea natalis | ? | - |
? | |
| additional information | glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds | Gecarcoidea natalis Pocock 1888 | ? | - |
? | |
| additional information | glucanase hydrolyses beta-1,4-glycosidic bonds | Cherax destructor Clark 1936 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,4-beta-glucanase 1 | - |
Cherax destructor |
| endo-1,4-beta-glucanase 2 | - |
Cherax destructor |
| endo-beta-1,4-glucanase | - |
Gecarcoidea natalis |
| endo-beta-1,4-glucanase 1 | - |
Cherax destructor |
| endo-beta-1,4-glucanase 2 | - |
Cherax destructor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Gecarcoidea natalis |
| 5.5 | - |
endo-beta-1,4-glucanase 1 | Cherax destructor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Gecarcoidea natalis |
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