3.2.1.4: cellulase This is an abbreviated version! For detailed information about cellulase, go to the full flat file .
Reaction
cellohexaose +
H2O =
2 cellotriose
Synonyms (1->4)-beta-D-glucan 4-glucanohydrolase, 1,4-beta-D-endoglucanase, 1,4-beta-D-glucan-4-glucanohydrolase, 168cel5, 9.5 cellulase, Abscission cellulase, AEG, Ag-EGase III, AgCMCase, alkali cellulase, Alkaline cellulase, AnCel5A, AtCel5, ATEG_07420, avicelase, BC-EG70a, Bc22Cel, BCE1, BcsZ, beta-1,4-endoglucan hydrolase, beta-1,4-endoglucanase, beta-1,4-glucanase, Bgl7A, bifunctional endoglucanase/xylanase, BlCel9, BP_Cel9A, Bx-ENG-1, Bx-ENG-2, Bx-ENG-3, C4endoII, carbomethyl cellulase, Carboxymethyl cellulase, Carboxymethyl-cellulase, carboxymethylcellulase, Cat 1, Caylase, CBH45-1, cbh6A, CBHI, CBHII, CcCel6C, CEL1, Cel1 EGase, Cel12A, Cel1753, Cel28a, Cel44A, Cel45A, Cel48A, Cel5, Cel5A, cel5B, Cel5E, Cel6A, Cel6A (E2), Cel6B, Cel6C, CEL7, Cel7A, Cel7B, Cel8, Cel8A, Cel8M, Cel8Y, Cel9A, CEL9A-50, CEL9A-65, Cel9A-68, CEL9A-82, Cel9A-90, Cel9B, CEL9C1, Cel9K, Cel9M, Cel9Q, CelA, CelB, CelC2 cellulase, CelCM3, CelDR, CelE, CelF, Celf_1230, Celf_3184, CelG, CelG endoglucanase, CelI15, cell-bound bacterial cellulase, CelL15, CelL73, cellic Ctec2, cellobiohydrolase, cellobiohydrolase I, celluase A, Celluclast, celludextrinase, Cellulase, cellulase 12A, cellulase A, cellulase A 3, cellulase Cel48F, cellulase Cel9A, cellulase Cel9M, cellulase CelC2, cellulase CelE, cellulase CM3, Cellulase E1, Cellulase E2, Cellulase E4, Cellulase E5, cellulase EGX, cellulase II, cellulase III, cellulase K, Cellulase SS, cellulase T, Cellulase V1, cellulase Xf818, cellulases I, cellulases III, cellulosin AP, Cellulysin, CelP, celS, CelStrep, celVA, CelX, CenA, CenC, CfCel6A, CfCel6C, CfEG3a, CHU_1280, CHU_2103, CjCel9A, Clocel_2741, CMCase, CMCase-I, CMCax, CMcellulase, Csac_1076, Csac_1078, CSCMCase, ctCel9D-Cel44A, CTendo45, ctendo7, CtGH5, Cthe_0435, CTHT_0045780, CX-cellulase, CyPB, DCC85_10145, DK-85, Dockerin type 1, Dtur_0671, E1 endoglucanase, Econase, EfPh, EG I, EG III, EG1, EG12, EG2, EG25, EG271, EG28, EG3, EG35, EG44, EG47, EG51, Eg5a, EG60, EGA, EGase, EGase II, EGB, EGC, EGCCA, EGCCC, EGCCD, EGCCF, EGCCG, EGD, EGE, EGF, egGH45, EGH, EGI, EGII, EGII/Cel5A, EGIV, EGL, EGL 1, Egl-257, Egl1, Egl499, Egl5a, EglA, eglB, EglC, EGLII, EglS, EGM, EGPf, EGPh, EGSS, EgV, EGX, EGY, EGZ, endo-1,4-B-glucanase, endo-1,4-beta-D-glucanase, endo-1,4-beta-glucanase, endo-1,4-beta-glucanase 1, endo-1,4-beta-glucanase 2, endo-1,4-beta-glucanase E1, endo-1,4-beta-glucanase V1, endo-beta-1,3-1,4-glucanase, endo-beta-1,4-glucanase, endo-beta-1,4-glucanase 1, endo-beta-1,4-glucanase 2, endo-beta-1,4-glucanase CMCax, endo-beta-1,4-glucanase EG27, endo-beta-1,4-glucanase EG45, endo-beta-D-1,4-glucanohydrolase, endo-beta-glucanase, endo-glucanase, ENDO1, ENDO2, endocellulase, endocellulase E1, endocellulases I, endocellulases II, endocellulases III, endocellulases IV, endogenous beta-1,4-endoglucanase, endogenous cellulase, endoglucanase, endoglucanase 1, endoglucanase 35, endoglucanase 47, endoglucanase CBP105, endoglucanase Cel 12A, endoglucanase Cel 5A, endoglucanase Cel 7B, endoglucanase Cel5A, endoglucanase Cel6A, endoglucanase D, endoglucanase EG-I, endoglucanase EG25, endoglucanase EG28, endoglucanase EG44, endoglucanase EG47, endoglucanase EG51, endoglucanase EG60, endoglucanase H, endoglucanase II, endoglucanase IIa, endoglucanase IV, endoglucanase L, endoglucanase M, endoglucanase V, endoglucanase Y, endolytic cellulase, EngA, EngH, EngL, EngM, engXCA, EngY, EngZ, family 7 cellobiohydrolase, FI-CMCASE, FnCel5A, FpCel45, Fpcel45a, GE40, GE40 endoglucanase, GH12 endo-1,4-beta-glucanase, GH124 endoglucanase, GH45 endoglucanase, gh45-1, GH5 cellulase, GH5 endoglucanase, GH6 endoglucanase, GH7 endoglucanase, GH9 termite cellulase, Glu1, Glu2, glycoside hydrolase family 9 endoglucanase, GtGH45, Lp-egl-1, manganese dependent endoglucanase, Maxazyme, Meicelase, mesophilic endoglucanase, mgCel6A, More, MtGH45, nmGH45, Onozuka R10, pancellase SS, PaPopCel1, PF0854, PH1171, PttCel9A, RCE1, RCE2, Roth 3056, RtGH124, Rucel5B, Sl-cel7, Sl-cel9C1, SnEG54, SoCel5, ssgluc, SSO1354, SSO1354 enzyme, SSO1354 protein, SSO1949, SSO2534, STCE1, Sumizyme, T12-GE40, TC Serva, TeEG-I, TeEgl5A, TfCel9A, ThEG, theme C glycoside hydrolase family 9 endo-beta-glucanase, Thermoactive cellulase, thermostable carboxymethyl cellulase, THITE_2110957, TM_1525, TM_1751, umcel5G, umcel9y-1, Vul_Cel5A
ECTree
Turnover Number
Turnover Number on EC 3.2.1.4 - cellulase
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0.358 - 0.397
2',4'-dinitrophenyl-beta-D-cellobiose
0.02 - 0.7
2,4-dinitrophenyl beta-D-cellobioside
0.46 - 0.6
4-methylumbelliferyl beta-D-lactoside
0.001 - 0.01
4-methylumbelliferyl cellobioside
0.79 - 9.55
4-nitrophenyl cellobioside
1.4
4-nitrophenyl cellopentaoside
pH 6.0, 95°C
3.6
4-nitrophenyl cellotetraoside
pH 6.0, 95°C
8.3
4-nitrophenyl cellotrioside
pH 6.0, 95°C
0.0178 - 466
4-nitrophenyl D-cellobioside
0.0007
barley beta-1,4-D-glucan
Thermochaetoides thermophila
recombinant His-tagged enzyme, pH 5.0, 55°C
-
127 - 295000
Barley beta-glucan
0.0007
beta-1,4-D-glucan
Thermochaetoides thermophila
pH 5.0, 55°C
0.00158
beta-1,4-D-xylan
Thermochaetoides thermophila
pH 5.0, 55°C
0.0013 - 677
carboxymethyl cellulose
-
0.094 - 1020
carboxymethylcellulose
-
0.095
cellobiose
pH 5.0, 60°C
41.7
cellohexaose
pH 7.0, 37°C
42.3
cellopentaose
pH 7.0, 37°C
159
cellotetraose
pH 7.0, 37°C
897
cellotriose
pH 7.0, 37°C
185.6
hydroxyethylcellulose
pH 7.0, 37°C
127.5
Laminarin
pH 7.0, 37°C
0.39 - 19
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
0.003 - 0.91
p-nitrophenyl cellobiose
0.01 - 0.18
p-nitrophenyl-beta-D-cellobioside
4260 - 26100
phosphoric acid swollen cellulose
-
0.092
raffinose
pH 5.0, 60°C
additional information
additional information
-
0.358
2',4'-dinitrophenyl-beta-D-cellobiose
-
chimeric xylanase/endoglucanase
0.397
2',4'-dinitrophenyl-beta-D-cellobiose
-
endoglucanase A
0.02
2,4-dinitrophenyl beta-D-cellobioside
50°C, wild-type enzyme Cel9A-68
0.07
2,4-dinitrophenyl beta-D-cellobioside
50°C, mutant enzyme E424A
0.7
2,4-dinitrophenyl beta-D-cellobioside
50°C, mutant enzyme E424G
0.46
4-methylumbelliferyl beta-D-lactoside
mutant G4C/A72C/N54C/P191C/T243C/A375C, pH 5.0, 22°C
0.47
4-methylumbelliferyl beta-D-lactoside
mutant T243C/A375C, pH 5.0, 22°C
0.5
4-methylumbelliferyl beta-D-lactoside
mutant G4C/A72C, pH 5.0, 22°C
0.52
4-methylumbelliferyl beta-D-lactoside
wild-type, pH 5.0, 22°C
0.55
4-methylumbelliferyl beta-D-lactoside
deglycosylated wild-type, pH 5.0, 22°C
0.6
4-methylumbelliferyl beta-D-lactoside
mutant N54C/P191C, pH 5.0, 22°C
0.001
4-methylumbelliferyl cellobioside
mutant K272F, pH 5.0, 45°C
0.001
4-methylumbelliferyl cellobioside
mutant Q274V, pH 5.0, 45°C
0.003
4-methylumbelliferyl cellobioside
mutant Q126F, pH 5.0, 45°C
0.01
4-methylumbelliferyl cellobioside
wild-type, pH 5.0, 45°C
0.79
4-nitrophenyl cellobioside
pH 6.0, 95°C
9.55
4-nitrophenyl cellobioside
pH 6.0, 70°C
0.0178
4-nitrophenyl D-cellobioside
N-glycosylation mutant residue D45, pH 4.8, 45°C
0.018
4-nitrophenyl D-cellobioside
N-glycosylation mutant residue D270, pH 4.8, 45°C
0.019
4-nitrophenyl D-cellobioside
wild-type, pH 4.8, 45°C
0.02
4-nitrophenyl D-cellobioside
N-glycosylation mutant residue 384, pH 4.8, 45°C
466
4-nitrophenyl D-cellobioside
pH 7.0, 37°C
0.091
Avicel
pH 5.0, 60°C
-
37.2
Avicel
pH 5.0, 50°C
-
127
Barley beta-glucan
-
mutant N19A, pH 5.0, 40°C
165
Barley beta-glucan
-
wild-type, pH 5.0, 40°C
227
Barley beta-glucan
-
mutant N42A, pH 5.0, 40°C
275
Barley beta-glucan
-
mutant N194A, pH 5.0, 40°C
311
Barley beta-glucan
pH 4.5, 90°C
3276
Barley beta-glucan
pH 7.0, 37°C
128000
Barley beta-glucan
pH 6.0, 50°C, recombinant enzyme
295000
Barley beta-glucan
pH 5.5, 65°C, recombinant enzyme
0.045
beta-Glucan
-
pH 4.2, 60°C, recombinant mutant CtGH1-CtGH5-F194A
0.09
beta-Glucan
-
pH 4.2, 60°C, recombinant wild-type enzyme CtGH5
0.135
beta-Glucan
-
pH 4.2, 60°C, recombinant mutant CtGH5-F194A
3.8
beta-Glucan
-
50°C, pH 5.5, endoglucanase EG25
16
beta-Glucan
-
50°C, pH 5.5, endoglucanase EG47
18
beta-Glucan
-
50°C, pH 5.5, endoglucanase EG60
64
beta-Glucan
-
50°C, pH 5.5, endoglucanase EG28
76
beta-Glucan
-
50°C, pH 5.5, endoglucanase EG44
84
beta-Glucan
-
50°C, pH 5.5, endoglucanase EG51
156
beta-Glucan
pH 5.0, 50°C
791
beta-Glucan
wild-type, pH 5.0, 85°C
1155
beta-Glucan
mutant Y61G, pH 5.0, 85°C
0.0013
carboxymethyl cellulose
-
pH 7.0, 37°C, mutant D392A
-
0.00211
carboxymethyl cellulose
Thermochaetoides thermophila
pH 5.0, 55°C
-
0.00211
carboxymethyl cellulose
Thermochaetoides thermophila
recombinant His-tagged enzyme, pH 5.0, 55°C
-
0.0051
carboxymethyl cellulose
-
pH 7.0, 37°C, mutant D392S
-
0.0059
carboxymethyl cellulose
-
pH 7.0, 37°C, mutant D392N
-
0.006
carboxymethyl cellulose
-
pH 4.2, 60°C, recombinant wild-type enzyme CtGH5
-
0.0073
carboxymethyl cellulose
-
pH 7.0, 37°C, mutant D252C
-
0.014
carboxymethyl cellulose
-
pH 7.0, 37°C, oxidized mutant D252C
-
0.0146
carboxymethyl cellulose
-
pH 7.0, 37°C, mutant D216C
-
0.026
carboxymethyl cellulose
-
pH 4.2, 60°C, recombinant mutant CtGH5-F194A
-
0.029
carboxymethyl cellulose
-
pH 7.0, 37°C, oxidized mutant D216C
-
0.115
carboxymethyl cellulose
-
pH 4.2, 60°C, recombinant mutant CtGH1-CtGH5-F194A
-
0.227
carboxymethyl cellulose
-
pH 7.0, 37°C, mutant D216N
-
0.994
carboxymethyl cellulose
-
pH 7.0, 37°C, mutant D392C
-
3.57
carboxymethyl cellulose
-
pH 7.0, 37°C, oxidized mutant D392C
-
4.2
carboxymethyl cellulose
-
pH 5.5, 45°C, recombinant enzyme
-
5.81
carboxymethyl cellulose
-
pH 7.0, 37°C, H2O2-treated wild-type CenA
-
5.92
carboxymethyl cellulose
-
pH 7.0, 37°C, wild-type CenA
-
7.4
carboxymethyl cellulose
-
in presence of 25 mM
-
15
carboxymethyl cellulose
-
50°C, pH 5.5, endoglucanase EG25
-
19
carboxymethyl cellulose
-
50°C, pH 5.5, endoglucanase EG28
-
22
carboxymethyl cellulose
-
50°C, pH 5.5, endoglucanase EG60
-
26
carboxymethyl cellulose
presence of 2.5 M NaCl, pH 5.0, 55°C
-
27
carboxymethyl cellulose
-
50°C, pH 5.5, endoglucanase EG47
-
49.2
carboxymethyl cellulose
pH 7.0, 37°C
-
51.6
carboxymethyl cellulose
pH 4.5, 90°C
-
54
carboxymethyl cellulose
-
50°C, pH 5.5, endoglucanase EG44
-
54
carboxymethyl cellulose
-
50°C, pH 5.5, endoglucanase EG51
-
70.6
carboxymethyl cellulose
pH 5.0, 50°C
-
74.51
carboxymethyl cellulose
recombinant enzyme, pH 5.0, 60°C
-
84.4
carboxymethyl cellulose
-
in presence of 250 mM
-
163
carboxymethyl cellulose
Rucel5B, pH 6.5, 60°C
-
190.1
carboxymethyl cellulose
-
in presence of 25 mM
-
209
carboxymethyl cellulose
-
pH 6.0, 80°C
-
217
carboxymethyl cellulose
-
enzyme from strain NIAB442
-
278
carboxymethyl cellulose
-
enzyme from deoxyglucose- and streptomycin-resistant mutant 51 Smr
-
298
carboxymethyl cellulose
-
pH 6.0, 85°C
-
318
carboxymethyl cellulose
-
in presence of 1.5 mM Mn2+
-
356
carboxymethyl cellulose
-
pH 6.0, 90°C
-
471
carboxymethyl cellulose
-
pH 6.0, 95°C
-
475
carboxymethyl cellulose
-
absence of Mn2+, pH 5.5, 60°C
-
677
carboxymethyl cellulose
-
presence of Mn2+, pH 5.5, 60°C
-
0.094
carboxymethylcellulose
pH 5.0, 60°C
-
0.15
carboxymethylcellulose
mutant V9A/K353E, pH 7.0, 40°C
-
0.16
carboxymethylcellulose
wild-type, pH 7.0, 40°C
-
0.17
carboxymethylcellulose
mutant G117S, pH 7.0, 40°C
-
0.75
carboxymethylcellulose
wild-type, pH 7.5, 65°C
-
2.2
carboxymethylcellulose
mutant lacking the IgG-like domain, pH 7.5, 65°C
-
4.6
carboxymethylcellulose
pH 7.0, 45°C
-
26.4
carboxymethylcellulose
-
pH 8.5, 40°C
-
246
carboxymethylcellulose
-
wild-type
-
437
carboxymethylcellulose
-
the turnover rate (kcat) of Egl330 increased by 78% compared to wild-type
-
1020
carboxymethylcellulose
-
pH 10, 50°C
-
0.053
lichenan
-
pH 4.2, 60°C, recombinant chimeric mutant CtGH1-CtGH5-F194A
0.1
lichenan
-
pH 4.2, 60°C, recombinant wild-type enzyme CtGH5
0.145
lichenan
-
pH 4.2, 60°C, recombinant mutant CtGH5-F194A
0.39
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
0.39
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 3, 80°C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
0.39
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH and temperature not specified in the publication, hybrid protein (CelA-SSO1949-CelA, consisting of 262 amino acids) based on beta-endoglucanase from Sulfolobus solfataricus P2 and beta-endoglucanase from Thermotoga maritima
19
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
pH 3, 80°C, native enzyme
0.003
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme I162A
0.006
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme H155A
0.01
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme E201Q
0.01
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme H297N
0.01
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme Y299F
0.036
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme E163A
0.037
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme P164A
0.083
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme R156A
0.092
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme G158A
0.11
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme D385N
0.115
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme I157A
0.129
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme T160A
0.144
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme C159A
0.157
p-nitrophenyl cellobiose
pH 5.5, 50°C, wild-type enzyme
0.167
p-nitrophenyl cellobiose
pH 5.5, 50°C, mutant enzyme H161A
0.24
p-nitrophenyl cellobiose
50°C, mutant enzyme C106A/C159A
0.41
p-nitrophenyl cellobiose
50°C, wild-type enzyme
0.41
p-nitrophenyl cellobiose
pH 5-6, 50°C, wild-type enzyme
0.43
p-nitrophenyl cellobiose
50°C, mutant enzyme C372A/C412A
0.47
p-nitrophenyl cellobiose
50°C, mutant enzyme C106A/C159A/C372A/C412A
0.75
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus
0.78
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme DELTAQ1-G5
0.91
p-nitrophenyl cellobiose
pH 5-6, 50°C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus
0.01
p-nitrophenyl-beta-D-cellobioside
-
-
0.18
p-nitrophenyl-beta-D-cellobioside
-
-
4260
phosphoric acid swollen cellulose
pH 6.0, 50°C, recombinant enzyme
-
26100
phosphoric acid swollen cellulose
pH 5.5, 65°C, recombinant enzyme
-
additional information
additional information
-
-
-
additional information
additional information
-
356 1/s, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose
-
additional information
additional information
-
kinetic analysis shows both the turnover rate (kcat) and the catalytic efficiency (kcat/Km) of Egl330 increased for the substrate carboxymethylcellulose compared to Egl499
-
additional information
additional information
kcat-values of N-terminal and C-terminal deletion mutants
-
additional information
additional information
-
kcat-values of N-terminal and C-terminal deletion mutants
-