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Literature summary for 3.2.1.4 extracted from

  • Liu, Y.; Dun, B.; Shi, P.; Ma, R.; Luo, H.; Bai, Y.; Xie, X.; Yao, B.
    A novel GH7 endo-beta-1,4-glucanase from Neosartorya fischeri P1 with good thermostability, broad substrate specificity and potential application in the brewing industry (2015), PLoS ONE, 10, e0137485.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
degradation under simulated mashing conditions, addition of Cel7A (99 microg) reduces the mash viscosity by 9.1% and filtration time by 24.6% Aspergillus fischeri

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Aspergillus fischeri

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 5 mM, no residual activity Aspergillus fischeri
Co2+ 5 mM, 90% of initial activity Aspergillus fischeri
EDTA 5 mM, 76% of initial activity Aspergillus fischeri
Mn2+ 5 mM, 44% of initial activity Aspergillus fischeri
Na+ 5 mM, 65% of initial activity Aspergillus fischeri
Pb2+ 5 mM, 56% of initial activity Aspergillus fischeri
sodium dodecylsulfate 5 mM, 5% of initial activity Aspergillus fischeri

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 119% of initial activity Aspergillus fischeri
Fe3+ 5 mM, 132% of initial activity Aspergillus fischeri

Organism

Organism UniProt Comment Textmining
Aspergillus fischeri A0A0N7E7X2 i.e. Aspergillus fischeri
-
Aspergillus fischeri P1 A0A0N7E7X2 i.e. Aspergillus fischeri
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a putative signal peptide at residues 1-20 Aspergillus fischeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2020
-
substrate barley beta-glucan, pH 5.0, 60°C Aspergillus fischeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
barley beta-glucan + H2O
-
Aspergillus fischeri ?
-
?
barley beta-glucan + H2O
-
Aspergillus fischeri P1 ?
-
?
carboxymethyl cellulose + H2O 18.6% of the activity with barley beta-glucan Aspergillus fischeri ?
-
?
carboxymethyl cellulose + H2O 18.6% of the activity with barley beta-glucan Aspergillus fischeri P1 ?
-
?
laminarin + H2O 9.1% of the activity with barley beta-glucan Aspergillus fischeri ?
-
?
laminarin + H2O 9.1% of the activity with barley beta-glucan Aspergillus fischeri P1 ?
-
?
lichenan + H2O 53.2% of the activity with barley beta-glucan Aspergillus fischeri ?
-
?
lichenan + H2O 53.2% of the activity with barley beta-glucan Aspergillus fischeri P1 ?
-
?
additional information enzyme displays weak activity on weak on locust bean galactomannan, avicel, and filter paper Aspergillus fischeri ?
-
?
additional information enzyme displays weak activity on weak on locust bean galactomannan, avicel, and filter paper Aspergillus fischeri P1 ?
-
?

Synonyms

Synonyms Comment Organism
Cel7A
-
Aspergillus fischeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Aspergillus fischeri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable Aspergillus fischeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Aspergillus fischeri

pH Range

pH Minimum pH Maximum Comment Organism
3 6
-
Aspergillus fischeri

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 stable Aspergillus fischeri