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Literature summary for 3.2.1.4 extracted from

  • Huang, Y.; Krauss, G.; Cottaz, S.; Driguez, H.; Lipps, G.
    A highly acid-stable and thermostable endo-beta-glucanase from the thermoacidophilic archaeon Sulfolobus solfataricus (2005), Biochem. J., 15, 581-588 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally truncated SSO1949 in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate pH 1.8, 80°C Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular presence of a signal peptide Saccharolobus solfataricus
-
-

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97X08 member of glycoside hydrolase family 12
-
Saccharolobus solfataricus DSM 1617 Q97X08 member of glycoside hydrolase family 12
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1
-
 pH 1.8, 80°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
 Saccharolobus solfataricus cellobiose + cellotriose + ?
-
 ?
carboxymethyl cellulose + H2O
-
 Saccharolobus solfataricus DSM 1617 cellobiose + cellotriose + ?
-
 ?
cellohexaose + H2O
-
 Saccharolobus solfataricus cellobiose + cellotriose
-
 ?
cellohexaose + H2O
-
 Saccharolobus solfataricus DSM 1617 cellobiose + cellotriose
-
 ?
cellopentaose + H2O
-
 Saccharolobus solfataricus cellobiose + cellotriose
-
 ?
cellopentaose + H2O
-
 Saccharolobus solfataricus DSM 1617 cellobiose + cellotriose
-
 ?
cellotetraose + H2O
-
 Saccharolobus solfataricus 2 cellobiose
-
 ?
cellotetraose + H2O
-
 Saccharolobus solfataricus DSM 1617 2 cellobiose
-
 ?
additional information no substrates: cellotriose, cellobiose, p-nitrophenyl beta-D-cellobioside and p-nitrophenyl beta-D-cellotrioside, xylan, laminarin and lichenan, crystalline cellulose Saccharolobus solfataricus ?
-
 ?
additional information no substrates: cellotriose, cellobiose, p-nitrophenyl beta-D-cellobioside and p-nitrophenyl beta-D-cellotrioside, xylan, laminarin and lichenan, crystalline cellulose Saccharolobus solfataricus DSM 1617 ?
-
 ?
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O
-
 Saccharolobus solfataricus ? + 5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE, x * 37508, calculated Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SSO1949
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 pH 1.8, half-life 8 h Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8
-
-
 Saccharolobus solfataricus

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.8
-
 80°C, half-life 8 h Saccharolobus solfataricus