Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.1%, 179% of initial activity | Bacillus vallismortis | |
dithiothreitol | 5 mM, 115% of initial activity | Bacillus vallismortis | |
EDTA | 5 mM, 121% of initial activity | Bacillus vallismortis | |
iodoacetic acid | 5 mM, 105% of initial activity | Bacillus vallismortis | |
Urea | 5 mM, 119% of initial activity | Bacillus vallismortis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 5 mM, 90% of initial activity | Bacillus vallismortis | |
Hg2+ | 5 mM, 46% residual activity | Bacillus vallismortis | |
Mn2+ | 5 mM, 77% residual activity | Bacillus vallismortis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
carboxymethyl cellulose | Km value 1.9 mg/ml, pH 7.0, 65°C | Bacillus vallismortis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 156% of initial activity | Bacillus vallismortis | |
Fe2+ | 5 mM, 113% of initial activity | Bacillus vallismortis | |
Mg2+ | 5 mM, 146% of initial activity | Bacillus vallismortis | |
Na+ | 5 mM, 128% of initial activity | Bacillus vallismortis | |
Ni2+ | 5 mM, 121% of initial activity | Bacillus vallismortis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Bacillus vallismortis |
Organic Solvent | Comment | Organism |
---|---|---|
benzene | 30%, 1 h, no change in activity, 72 h, 109% of initial activity | Bacillus vallismortis |
Butanol | 30%, 1 h, 121% of initial activity, 72 h, 122% of initial activity | Bacillus vallismortis |
isopropanol | 30%, 1 h, 91% residual activity, 72 h, 92% residual activity | Bacillus vallismortis |
Methanol | 30%, 1 h, no change in activity, 72 h, 125% of initial activity | Bacillus vallismortis |
n-decane | 30%, 1 h, 135% of initial activity, 72 h, 158% of initial activity | Bacillus vallismortis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus vallismortis | - |
- |
- |
Bacillus vallismortis RG-07 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus vallismortis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
123600 | - |
pH 7.0, 65°C | Bacillus vallismortis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
Bacillus vallismortis | ? | - |
? | |
carboxymethyl cellulose + H2O | - |
Bacillus vallismortis RG-07 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Bacillus vallismortis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Bacillus vallismortis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
95% of maximum activity | Bacillus vallismortis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus vallismortis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | - |
75% of maximum activtiy | Bacillus vallismortis |
Organism | Comment | Expression |
---|---|---|
Bacillus vallismortis | maximum cellulase production upon growth on sugarcane baggase used as agro-waste carbon source | additional information |