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Literature summary for 3.2.1.4 extracted from

  • Gaur, R.; Tiwari, S.
    Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07 (2015), BMC Biotechnol., 15, 19.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 0.1%, 179% of initial activity Bacillus vallismortis
dithiothreitol 5 mM, 115% of initial activity Bacillus vallismortis
EDTA 5 mM, 121% of initial activity Bacillus vallismortis
iodoacetic acid 5 mM, 105% of initial activity Bacillus vallismortis
Urea 5 mM, 119% of initial activity Bacillus vallismortis

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 5 mM, 90% of initial activity Bacillus vallismortis
Hg2+ 5 mM, 46% residual activity Bacillus vallismortis
Mn2+ 5 mM, 77% residual activity Bacillus vallismortis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
carboxymethyl cellulose Km value 1.9 mg/ml, pH 7.0, 65°C Bacillus vallismortis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 156% of initial activity Bacillus vallismortis
Fe2+ 5 mM, 113% of initial activity Bacillus vallismortis
Mg2+ 5 mM, 146% of initial activity Bacillus vallismortis
Na+ 5 mM, 128% of initial activity Bacillus vallismortis
Ni2+ 5 mM, 121% of initial activity Bacillus vallismortis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Bacillus vallismortis

Organic Solvent Stability

Organic Solvent Comment Organism
benzene 30%, 1 h, no change in activity, 72 h, 109% of initial activity Bacillus vallismortis
Butanol 30%, 1 h, 121% of initial activity, 72 h, 122% of initial activity Bacillus vallismortis
isopropanol 30%, 1 h, 91% residual activity, 72 h, 92% residual activity Bacillus vallismortis
Methanol 30%, 1 h, no change in activity, 72 h, 125% of initial activity Bacillus vallismortis
n-decane 30%, 1 h, 135% of initial activity, 72 h, 158% of initial activity Bacillus vallismortis

Organism

Organism UniProt Comment Textmining
Bacillus vallismortis
-
-
-
Bacillus vallismortis RG-07
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus vallismortis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
123600
-
pH 7.0, 65°C Bacillus vallismortis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl cellulose + H2O
-
Bacillus vallismortis ?
-
?
carboxymethyl cellulose + H2O
-
Bacillus vallismortis RG-07 ?
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Bacillus vallismortis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Bacillus vallismortis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
95
-
95% of maximum activity Bacillus vallismortis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus vallismortis

pH Range

pH Minimum pH Maximum Comment Organism
9
-
75% of maximum activtiy Bacillus vallismortis

Expression

Organism Comment Expression
Bacillus vallismortis maximum cellulase production upon growth on sugarcane baggase used as agro-waste carbon source additional information