Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | uncultured bacterium |
Crystallization (Comment) | Organism |
---|---|
to 2.15 A resolution. Residues Asp194 and Asp197 form hydrogen bonds with a water molecule that may function as a nucleophile | uncultured bacterium |
Protein Variants | Comment | Organism |
---|---|---|
D99A | 106% of wild-type activity, temperature of the midpoint of the thermal denaturation transition is decreased by 1.9°C | uncultured bacterium |
additional information | a mutant lacking the immunoglobulin-like domain shows 1% of wild-type activity and a decrease in the temperature of the midpoint of the thermal denaturation transition by 6.3°C | uncultured bacterium |
Q40A | 94% of wild-type activity | uncultured bacterium |
Q40A/D99A | 97% of wild-type activity, temperature of the midpoint of the thermal denaturation transition is decreased by 5.0°C | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
gel filtration | uncultured bacterium |
62600 | - |
1 * 62600, calculated | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | W8PF21 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.4 | - |
wild-type, pH 7.0, 50°C | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl cellulose + H2O | - |
uncultured bacterium | ? | - |
? | |
additional information | no substrate: 4-nitrophenyl cellobioside | uncultured bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62600, calculated | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
CelG | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
81.4 | - |
denaturation temperature | uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | more than 80% of maximum activity | uncultured bacterium |