Information on EC 3.2.1.4 - cellulase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Xylella fastidiosa

EC NUMBER
COMMENTARY
3.2.1.4
-
RECOMMENDED NAME
GeneOntology No.
cellulase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
show the reaction diagram
-
-
-
-
endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
show the reaction diagram
proposed mechanism of action of inverting glycoside hydrolases and structures of catalytic residues, overview. The catalytic base is Asp392, catalytic acid residue is Asp252
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis
-
-
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
cellulose degradation II (fungi)
-
Metabolic pathways
-
Starch and sucrose metabolism
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan 4-glucanohydrolase
Will also hydrolyse 1,4-linkages in beta-D-glucans also containing 1,3-linkages.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,4-beta-D-endoglucanase
Q83XK5
-
1,4-beta-D-endoglucanase
Rhizobium leguminosarum bv. trifolii ANU843
Q83XK5
-
-
1,4-beta-D-glucan-4-glucanohydrolase
-
-
9.5 cellulase
-
-
-
-
Abscission cellulase
-
-
-
-
AEG
Gluconacetobacter xylinus
-
-
Ag-EGase III
Q5DIE3
-
alkali cellulase
-
-
-
-
Alkaline cellulase
-
-
-
-
Alkaline cellulase
-
-
Alkaline cellulase
Marinobacter sp. MSI032
-
-
-
avicelase
-
-
-
-
beta-1,4-endoglucan hydrolase
-
-
-
-
beta-1,4-endoglucanase
-
-
beta-1,4-endoglucanase
Bacillus sp. CTP-09
-
-
-
beta-1,4-endoglucanase
O58925
-
beta-1,4-endoglucanase
-
-
beta-1,4-endoglucanase
Q60032
-
beta-1,4-endoglucanase
G3BJX5
-
beta-1,4-glucanase
-
-
-
-
beta-1,4-glucanase
-
-
Bgl7A
D2DRB6
-
C4endoII
Trichoderma sp.
-
-
C4endoII
-
-
-
carbomethyl cellulase
-
-
Carboxymethyl cellulase
-
-
-
-
Carboxymethyl cellulase
-
-
Carboxymethyl cellulase
Acremonium cellulolyticus CF-2612
-
-
-
Carboxymethyl cellulase
-
-
Carboxymethyl cellulase
Bacillus sp. DUSELR13
-
-
-
Carboxymethyl cellulase
-
-
Carboxymethyl cellulase
A4UU22
-
Carboxymethyl cellulase
Geobacillus sp.
-
-
Carboxymethyl cellulase
Geobacillus sp. WSUCFI
-
-
-
Carboxymethyl cellulase
B5BNY1
-
Carboxymethyl cellulase
Staphylotrichum coccosporum NBRC 31817
B5BNY1
-
-
Carboxymethyl cellulase
-
-
Carboxymethyl-cellulase
-
-
-
-
carboxymethylcellulase
-
-
-
-
CEL1
-
-
-
-
Cel1 EGase
-
-
Cel1 EGase
Solanum lycopersicum Mill.
-
-
-
Cel12A
O00095
-
Cel12A
Q60032
-
Cel45A
Q8J0K8
-
Cel5A
Bacillus subtilis BME-15
-
-
-
Cel6A (E2)
-
-
Cel8A
Q25C15
-
Cel8A
Q25C15
-
-
CEL9A-50
A0ZT16
-
CEL9A-65
A0ZT16
-
Cel9A-68
P26221
Cel9A is secreted as a 90400 Da protein. Limited proteolysis of Cel9A produces Cel9A-68
Cel9A-68
Thermobifida fusca Cel9A
P26221
Cel9A is secreted as a 90400 Da protein. Limited proteolysis of Cel9A produces Cel9A-68
-
CEL9A-82
A0ZT16
-
Cel9A-90
P26221
Cel9A is secreted as a 90400 Da protein. Limited proteolysis of Cel9A produces Cel9A-68
Cel9A-90
Thermobifida fusca Cel9A
P26221
Cel9A is secreted as a 90400 Da protein. Limited proteolysis of Cel9A produces Cel9A-68
-
CelC2 cellulase
Q83XK5
-
CelC2 cellulase
Rhizobium leguminosarum bv. trifolii ANU843
Q83XK5
-
-
CelDR
A8D0T0
-
CelDR
Bacillus subtilis DR
A8D0T0
-
-
CelG
Alicyclobacillus acidocaldarius ATCC27009
-
-
-
CelG endoglucanase
-
-
CelI15
B7UAM4
-
CelI15
Bacillus subtilis I15
B7UAM4
-
-
cell-bound bacterial cellulase
Q83XK5
-
cell-bound bacterial cellulase
Rhizobium leguminosarum bv. trifolii ANU843
Q83XK5
-
-
CelL15
C5I946
-
CelL15
Bacillus subtilis LN
C5I946
-
-
CelL73
C5I945
-
CelL73
Bacillus subtilis LN
C5I945
-
-
cellobiohydrolase
-
-
-
-
cellobiohydrolase
-
-
celluase A
-
-
-
-
Celluclast
-
-
celludextrinase
-
-
-
-
Cellulase
-
-
-
-
Cellulase
-
-
Cellulase
A8D0T0
-
Cellulase
Bacillus subtilis BME-15
-
-
-
Cellulase
Bacillus subtilis DR
A8D0T0
-
-
Cellulase
Q9BLD2
-
Cellulase
A0ZT16
-
Cellulase
Q8J0K6, Q8J0K7, Q8J0K8
-
Cellulase
Q40763
-
Cellulase
Q97YG7
-
Cellulase
Sulfolobus solfataricus Oalpha
Q97YG7
-
-
Cellulase
-
-
Cellulase
Thermobifida fusca Cel6A
-
-
-
cellulase 12A
Q60032
-
cellulase A
-
-
cellulase A 3
-
-
-
-
cellulase Cel48F
-
-
cellulase Cel9A
-
-
cellulase Cel9M
-
shortest variant of family 9 cellulases which contains only the catalytic module to interact with the substrate
cellulase CelC2
Q83XK5
is essential for primary symbiotic infection of legume host roots
cellulase CelC2
Rhizobium leguminosarum bv. trifolii ANU843
Q83XK5
is essential for primary symbiotic infection of legume host roots
-
cellulase CelE
-
-
Cellulase E1
-
-
-
-
Cellulase E2
-
-
-
-
Cellulase E4
-
-
-
-
Cellulase E5
-
-
-
-
cellulase EGX
-
-
cellulase III
Q5DIE3
-
cellulase K
-
-
Cellulase SS
-
-
-
-
cellulase T
-
-
Cellulase V1
-
-
-
-
cellulase Xf818
Q9PF60
-
cellulosin AP
-
-
-
-
Cellulysin
-
-
celS
Sulfolobus solfataricus MT4
Q97VS7
-
-
CfEG3a
Q9BLD2
-
CjCel9A
A0ZT16
-
CMCase
-
-
-
-
CMCase
Acremonium cellulolyticus CF-2612
-
-
-
CMCase
Aspergillus oryzae cmc-1
-
-
-
CMCase
Bacillus sp. CTP-09, Bacillus sp. DUSELR13
-
-
-
CMCase
B7UAM4
-
CMCase
Bacillus subtilis I15
B7UAM4
-
-
CMCase
Geobacillus sp.
-
-
CMCase
Geobacillus sp. WSUCFI
-
-
-
CMCase
Marinobacter sp. MSI032
-
-
-
CMCase
Staphylotrichum coccosporum NBRC 31817
B5BNY1
-
-
CMCax
Gluconacetobacter xylinus
-
-
CSCMCase
A4UU22
-
ctCel9D-Cel44A
-
bifunctional multimodular cellulase
CX-cellulase
-
-
-
-
E1 endoglucanase
-
-
E1 endoglucanase
Acidothermus cellulolyticus E1
-
-
-
EG1
-
-
-
-
EG2
-
-
-
-
EG3
-
-
-
-
EG44
-
EG44 and RG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module
EG51
-
EG44 and RG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module
EGA
-
-
-
-
EGB
-
-
-
-
EGC
-
-
-
-
EGCCA
-
-
-
-
EGCCC
-
-
-
-
EGCCD
-
-
-
-
EGCCF
-
-
-
-
EGCCG
-
-
-
-
EGD
-
-
-
-
EGE
-
-
-
-
EGF
-
-
-
-
EGH
-
-
-
-
EGI
-
-
-
-
EGIV
-
-
-
-
EGL 1
-
-
-
Egl-257
Bacillus circulans KSM-N257
-
-
-
Egl499
Bacillus subtilis JA18
-
-
-
EGM
-
-
-
-
EGPf
E7FHY8
-
EGPf
Q9V2T0
-
EGPh
Pyrococcus horikoshii DSM 12428
O58925
;
-
EGSS
-
-
-
-
EgV
Q9LCC5
-
EgV
Ruminococcus albus F-40
Q9LCC5
-
-
EGX
-
-
-
-
EGY
-
-
-
-
EGZ
-
-
-
-
endo-1,4-beta-D-glucanase
-
-
-
-
endo-1,4-beta-D-glucanase
-
-
endo-1,4-beta-D-glucanase
-
-
endo-1,4-beta-glucanase
-
-
-
-
endo-1,4-beta-glucanase
-
-
endo-1,4-beta-glucanase
Q9V2T0
-
endo-1,4-beta-glucanase
-
-
endo-1,4-beta-glucanase
-
-
endo-1,4-beta-glucanase 1
-
-
endo-1,4-beta-glucanase 1
Cherax destructor Clark
-
-
-
endo-1,4-beta-glucanase 2
-
-
Endo-1,4-beta-glucanase E1
-
-
-
-
Endo-1,4-beta-glucanase V1
-
-
-
-
endo-beta-1,3-1,4-glucanase
D2DRB6
-
endo-beta-1,4-glucanase
-
-
endo-beta-1,4-glucanase
B7UAM4
-
endo-beta-1,4-glucanase
Bacillus subtilis BME-15
-
-
-
endo-beta-1,4-glucanase
Bacillus subtilis I15
B7UAM4
-
-
endo-beta-1,4-glucanase
Bacillus subtilis JA18
-
-
-
endo-beta-1,4-glucanase
-
-
endo-beta-1,4-glucanase
Gecarcoidea natalis Pocock 1888
-
-
-
endo-beta-1,4-glucanase
-
-
endo-beta-1,4-glucanase
O76130
-
endo-beta-1,4-glucanase
-
-
endo-beta-1,4-glucanase 1
-
-
endo-beta-1,4-glucanase 1
Cherax destructor Clark
-
-
-
endo-beta-1,4-glucanase 2
-
-
endo-beta-1,4-glucanase 2
Cherax destructor Clark
-
-
-
endo-beta-1,4-glucanase CMCax
Gluconacetobacter xylinus
-
-
endo-beta-1,4-glucanase EG27
-
-
endo-beta-1,4-glucanase EG45
-
-
endo-glucanase
-
-
endo-glucanase
Bacillus sp. CTP-09
-
-
-
endocellulase
A8D0T0
-
endocellulase
Bacillus subtilis DR
A8D0T0
-
-
endocellulase
O58925
-
endocellulase
-
-
Endocellulase E1
-
-
-
-
endogenous beta-1,4-endoglucanase
-
-
endogenous cellulase
A0ZT16
-
Endoglucanase
-
-
-
-
Endoglucanase
-
-
Endoglucanase
Aspergillus oryzae cmc-1
-
-
-
Endoglucanase
-
-
Endoglucanase
Bacillus sp. CTP-09
-
-
-
Endoglucanase
Brevibacillus sp.
-
-
Endoglucanase
-
-
-
Endoglucanase
Q6BCL3, Q6BCL7, Q6BCM1
-
Endoglucanase
Q9BLD2
-
Endoglucanase
A7HLZ2
-
Endoglucanase
-
-
Endoglucanase
Q8J0K6, Q8J0K7, Q8J0K8
-
Endoglucanase
-
-
Endoglucanase
-
-
Endoglucanase
B5BNY1
-
Endoglucanase
Staphylotrichum coccosporum NBRC 31817
B5BNY1
-
-
Endoglucanase
Thermobifida fusca Cel6A
-
-
-
endoglucanase 1
-
-
endoglucanase 1
-
-
endoglucanase 35
-
-
endoglucanase 47
-
-
endoglucanase CBP105
Q3ZMA8
-
endoglucanase CBP105
Cellulomonas flavigena 531
Q3ZMA8
-
-
endoglucanase Cel 12A
-
-
endoglucanase Cel 5A
-
-
endoglucanase Cel 7B
-
-
endoglucanase Cel5A
Q3LHN3
-
endoglucanase Cel6A
-
-
endoglucanase D
-
-
-
-
endoglucanase EG25
-
-
endoglucanase EG28
-
-
endoglucanase EG44
-
endoglucanase EG44 and endoglucanase EG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module
endoglucanase EG47
-
-
endoglucanase EG51
-
endoglucanase EG44 and endoglucanase EG51 are different forms of one enzyme. EG44 seems to be the catalytic module of an intact EG51 without a cellulose-binding module
endoglucanase EG60
-
-
endoglucanase H
O65987
-
endoglucanase II
-
-
endoglucanase IV
-
-
endoglucanase L
Q9RGE6
-
endoglucanase M
Q9LAJ2
-
endoglucanase V
Q9LCC5
-
endoglucanase V
Ruminococcus albus F-40
Q9LCC5
-
-
endoglucanase Y
Q9AQF4
-
endolytic cellulase
-
-
family 7 cellobiohydrolase
-
-
FI-CMCASE
-
-
-
-
GH9 termite cellulase
-
-
Maxazyme
-
-
Onozuka R10
-
-
pancellase SS
-
-
-
-
PaPopCel1
Q40763
-
PF0854
E7FHY8
gene name
PH1171
O58925
locus name
PH1171
Pyrococcus horikoshii DSM 12428
O58925
locus name; locus name
-
Roth 3056
-
-
Rucel5B
G3BJX5
-
SSO1354
Q97YG7
-
SSO1354
Q97YG7
locus name
SSO1354
Sulfolobus solfataricus Oalpha
Q97YG7
-
-
SSO1354
Q97YG7
locus name
-
SSO1354 enzyme
Q97YG7
-
SSO1354 protein
Q97YG7
-
SSO1354 protein
Sulfolobus solfataricus Oalpha
Q97YG7
-
-
SSO1949
Q97X08
locus name
SSO1949
Q97X08
locus name; locus name
-
STCE1
Staphylotrichum coccosporum NBRC 31817
B5BNY1
-
-
Sumizyme
-
-
TC Serva
-
-
ThEG
Q75UV6
-
thermoacidophilic cellulase
-
-
thermoacidophilic cellulase
Alicyclobacillus acidocaldarius ATCC27009
-
-
-
Thermoactive cellulase
-
-
-
-
thermostable carboxymethyl cellulase
A4UU22
-
Meicelase
-
-
additional information
D2DRB6
the enzyme belongs to the acidic glycoside hydrolase family 7, GH7
additional information
-
cellulase A is an inverting glycoside hydrolase and a member of family 6 of the CAZy database classification system
additional information
-
the enzyme belongs to the glycohydrolase family 9
additional information
-
the enzyme belongs to the glycoside hydrolase family 6
additional information
F6M085
the enzyme is a a member of the TIM barrel glycosyl hydrolase superfamily (beta/alpha)8
additional information
Desulfurococcaceae EBI-244
F6M085
the enzyme is a a member of the TIM barrel glycosyl hydrolase superfamily (beta/alpha)8
-
additional information
-
the enzyme belongs to the gylcosyl hydrolase family GH9
additional information
Q60032
the enzyme belongs to the GH12 family of glycoside hydrolases
CAS REGISTRY NUMBER
COMMENTARY
9012-54-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Acidothermus cellulolyticus E1
strain E1
-
-
Manually annotated by BRENDA team
filamentous fungus, strain C-1, contains different cellulolytic enzymes
-
-
Manually annotated by BRENDA team
Acremonium cellulolyticus CF-2612
-
-
-
Manually annotated by BRENDA team
Alicyclobacillus acidocaldarius ATCC27009
ATCC27009
-
-
Manually annotated by BRENDA team
multifunctional cellulase activity with endo-beta-1,4-glucanase activity, RC 3.2.1.4, exo-beta-1,4-glucanase activity, EC 3.2.9.11, and endo-beta-1,4-xylanase activity, EC 3.2.1.8
-
-
Manually annotated by BRENDA team
strain VF5
-
-
Manually annotated by BRENDA team
Aspergillus aculeatus NO. F-50
NO. F-50
-
-
Manually annotated by BRENDA team
IFO31125
-
-
Manually annotated by BRENDA team
Aspergillus niger IFO31125
IFO31125
-
-
Manually annotated by BRENDA team
strain ITCC-4857.01, isolated from rice bran
-
-
Manually annotated by BRENDA team
Aspergillus oryzae cmc-1
cmc-1
-
-
Manually annotated by BRENDA team
Aspergillus oryzae ITCC-4857.01
strain ITCC-4857.01, isolated from rice bran
-
-
Manually annotated by BRENDA team
Aspergillus terreus GN1
GN1
-
-
Manually annotated by BRENDA team
Bacillus agaradhaerens JAM-KU023
JAM-KU023
-
-
Manually annotated by BRENDA team
Bacillus amyloliquefaciens DL-3
strain DL-3
-
-
Manually annotated by BRENDA team
Bacillus cellulyticus K-12
K-12
-
-
Manually annotated by BRENDA team
KSM-N257
-
-
Manually annotated by BRENDA team
Bacillus circulans F-2
F-2
-
-
Manually annotated by BRENDA team
Bacillus circulans KSM-N257
KSM-N257
-
-
Manually annotated by BRENDA team
CTP-09
-
-
Manually annotated by BRENDA team
isolated from a cellulose-degrading enrichment culture initiated from compost samples
-
-
Manually annotated by BRENDA team
N-4 (NK1)
-
-
Manually annotated by BRENDA team
N-4; No. 1139
-
-
Manually annotated by BRENDA team
No. 1139
-
-
Manually annotated by BRENDA team
strain CH43; strain HR68
-
-
Manually annotated by BRENDA team
strain CH43
-
-
Manually annotated by BRENDA team
Bacillus sp. CTP-09
CTP-09
-
-
Manually annotated by BRENDA team
Bacillus sp. DUSELR13
isolated from a cellulose-degrading enrichment culture initiated from compost samples
-
-
Manually annotated by BRENDA team
Bacillus sp. HR68
strain HR68
-
-
Manually annotated by BRENDA team
Bacillus sp. HSH-810
-
-
-
Manually annotated by BRENDA team
Bacillus sp. N-4
N-4
-
-
Manually annotated by BRENDA team
Bacillus sp. N-4 (NK1)
N-4 (NK1)
-
-
Manually annotated by BRENDA team
Bacillus sp. No. 1139
No. 1139
-
-
Manually annotated by BRENDA team
CelL15; strain LN, isolated of feces sample of Golden Takin, Budorcas taxicolor, genes CelL15
UniProt
Manually annotated by BRENDA team
CelL73; strain LN, isolated of feces sample of Golden Takin, Budorcas taxicolor, gene CelL73
UniProt
Manually annotated by BRENDA team
DLG, N-24, or PAP115
-
-
Manually annotated by BRENDA team
strain I15, isolated from the longtime-thermal compost containing rich cellulose materials in a factory of Zhengzhou in China, gene celI15
UniProt
Manually annotated by BRENDA team
Bacillus subtilis AS3
-
-
-
Manually annotated by BRENDA team
Bacillus subtilis BME-15
BME-15
-
-
Manually annotated by BRENDA team
Bacillus subtilis CK-2
CK-2
-
-
Manually annotated by BRENDA team
Bacillus subtilis DR
DR
UniProt
Manually annotated by BRENDA team
Bacillus subtilis I15
strain I15, isolated from the longtime-thermal compost containing rich cellulose materials in a factory of Zhengzhou in China, gene celI15
UniProt
Manually annotated by BRENDA team
Bacillus subtilis JA18
JA18
-
-
Manually annotated by BRENDA team
Bacillus subtilis LN
CelL15; strain LN, isolated of feces sample of Golden Takin, Budorcas taxicolor, genes CelL15
UniProt
Manually annotated by BRENDA team
Bacillus subtilis LN
CelL73; strain LN, isolated of feces sample of Golden Takin, Budorcas taxicolor, gene CelL73
UniProt
Manually annotated by BRENDA team
acidophilic fungus, CGMCC 2500, gene bgl7A
UniProt
Manually annotated by BRENDA team
Brevibacillus sp.
strain JXL
-
-
Manually annotated by BRENDA team
strain JXL
-
-
Manually annotated by BRENDA team
anaerobic fungal isolate, is purified from bovine rumen
-
-
Manually annotated by BRENDA team
strain NIAB 442 and its deoxyglucose- and streptomycin-resistant mutant 51 Smr
-
-
Manually annotated by BRENDA team
Cellulomonas biazotea NIAB 442
strain NIAB 442 and its deoxyglucose- and streptomycin-resistant mutant 51 Smr
-
-
Manually annotated by BRENDA team
endoglucanase A and B
-
-
Manually annotated by BRENDA team
gene cenA
-
-
Manually annotated by BRENDA team
CDBB 531 strain
SwissProt
Manually annotated by BRENDA team
Cellulomonas flavigena 531
CDBB 531 strain
SwissProt
Manually annotated by BRENDA team
var. dissitum
-
-
Manually annotated by BRENDA team
Chaetomium thermophilum CT2
strain CT2
-
-
Manually annotated by BRENDA team
; strain Clark 1936
-
-
Manually annotated by BRENDA team
Cherax destructor Clark
strain Clark 1936
-
-
Manually annotated by BRENDA team
deletion variants CelEM1, CelEM2 and CelEM3
-
-
Manually annotated by BRENDA team
endoglucanase 1b and 2
-
-
Manually annotated by BRENDA team
enzyme form EGA, EGB, EGC and EGD
-
-
Manually annotated by BRENDA team
production of several endoglucanases, which are part of a cellulase complex termed cellulosome
-
-
Manually annotated by BRENDA team
Clostridium thermocellum CelJ
CelJ
-
-
Manually annotated by BRENDA team
isolated from a sample of a circumneutral geothermal pool at 94 C
UniProt
Manually annotated by BRENDA team
Desulfurococcaceae EBI-244
isolated from a sample of a circumneutral geothermal pool at 94 C
UniProt
Manually annotated by BRENDA team
endoglucanase Z and Y
-
-
Manually annotated by BRENDA team
endoglucanase F; S85
-
-
Manually annotated by BRENDA team
Fibrobacter succinogenes S85
S85
-
-
Manually annotated by BRENDA team
; strain Pocock 1888
-
-
Manually annotated by BRENDA team
Gecarcoidea natalis Pocock 1888
strain Pocock 1888
-
-
Manually annotated by BRENDA team
Geobacillus sp.
isolated from a cellulose-degrading enrichment culture initiated from compost samples
-
-
Manually annotated by BRENDA team
Geobacillus sp. WSUCFI
isolated from a cellulose-degrading enrichment culture initiated from compost samples
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus
-
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus
ATCC 23769
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
ATCC 26921
-
-
Manually annotated by BRENDA team
strain PC-3-7, gene egl3
-
-
Manually annotated by BRENDA team
strains QM6a and PC-3-7, genes egl1 and egl3
-
-
Manually annotated by BRENDA team
Hypocrea jecorina PC-3-7
strain PC-3-7, gene egl3
-
-
Manually annotated by BRENDA team
a low-molecular weight enzyme form and a high-molecular weight enzyme form
-
-
Manually annotated by BRENDA team
cellulase II-A and II-B
-
-
Manually annotated by BRENDA team
QM 9414, mutant strain
-
-
Manually annotated by BRENDA team
Hypocrea rufa QM 9414
QM 9414, mutant strain
-
-
Manually annotated by BRENDA team
i.e. Polyporus tulipiferae
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
Lenzites trabea
-
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
Lysinibacillus sphaericus JS1
strain JS1
-
-
Manually annotated by BRENDA team
strain IB-9374
SwissProt
Manually annotated by BRENDA team
strain IB-9374
SwissProt
Manually annotated by BRENDA team
Cavara, pathogenic strain T-1 (Ken53-33) and nonpathogenic strain O(THU3*1), strain C-3(N87), strain N-1(H373)
-
-
Manually annotated by BRENDA team
strain MSI032, isolated from the marine sponge Dendrilla nigra
-
-
Manually annotated by BRENDA team
Marinobacter sp. MSI032
strain MSI032, isolated from the marine sponge Dendrilla nigra
-
-
Manually annotated by BRENDA team
Melanocarpus albomyces ALKO4237
ALKO4237
-
-
Manually annotated by BRENDA team
NRRL 26519
-
-
Manually annotated by BRENDA team
Mucor circinelloides NRRL 26519
NRRL 26519
-
-
Manually annotated by BRENDA team
Myceliophthora heterothallica D-14
D-14
-
-
Manually annotated by BRENDA team
Penicillium brasilianum IBT 20888
IBT 20888
-
-
Manually annotated by BRENDA team
strain JUA10
-
-
Manually annotated by BRENDA team
Penicillium decumbens JUA10
strain JUA10
-
-
Manually annotated by BRENDA team
Penicillium funiculosum
-
-
-
Manually annotated by BRENDA team
Phytomonas davidi
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
strain DY3
-
-
Manually annotated by BRENDA team
Pseudoalteromonas sp. DY3
strain DY3
-
-
Manually annotated by BRENDA team
wild-type and catabolite respression resistant mutants CRRmt4 and CRRmt24
-
-
Manually annotated by BRENDA team
Pyrococcus horikoshii DSM 12428
-
UniProt
Manually annotated by BRENDA team
Pyrococcus horikoshii OT-3
-
UniProt
Manually annotated by BRENDA team
presence of two independent endogenous and symbiotic cellulolytic systems in flagellate-harboring termites
-
-
Manually annotated by BRENDA team
Rhizobium leguminosarum bv. trifolii ANU843
strain ANU843
UniProt
Manually annotated by BRENDA team
Robillarda sp.
Y-20
-
-
Manually annotated by BRENDA team
endoglucanase III
-
-
Manually annotated by BRENDA team
Ruminococcus albus F-40
F-40
SwissProt
Manually annotated by BRENDA team
Ruminococcus albus F40
F40
-
-
Manually annotated by BRENDA team
cv. Moneymaker
-
-
Manually annotated by BRENDA team
Solanum lycopersicum Mill.
Mill.
-
-
Manually annotated by BRENDA team
cellulase I and II
-
-
Manually annotated by BRENDA team
QMB 482, ATCC 10011
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
-
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
ATCC 32629
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
five endoglucanases
-
-
Manually annotated by BRENDA team
Stachybotrys atra
-
-
-
Manually annotated by BRENDA team
Staphylotrichum coccosporum NBRC 31817
NBRC 31817
UniProt
Manually annotated by BRENDA team
CMCase 1 and 2
-
-
Manually annotated by BRENDA team
Streptomyces rochei A2
A2
-
-
Manually annotated by BRENDA team
contains two different types of CMCases type I and II
-
-
Manually annotated by BRENDA team
Japanese purple sea urchin
-
-
Manually annotated by BRENDA team
; strains P2, MT4, and Oalpha
Q97YG7
UniProt
Manually annotated by BRENDA team
putative; MT4 strain
Q97VS7
SwissProt
Manually annotated by BRENDA team
Sulfolobus solfataricus MT4
putative; MT4 strain
Q97VS7
SwissProt
Manually annotated by BRENDA team
Sulfolobus solfataricus Oalpha
-
Q97YG7
UniProt
Manually annotated by BRENDA team
Syncephalastrum racemosum BCC18080
BCC18080
Uniprot
Manually annotated by BRENDA team
Talaromyces emersonii CBS 814.70
CBS814,70
-
-
Manually annotated by BRENDA team
a filamentous bacterium, strain ATCC BAA-629, genes Tfu_1627, Tfu_1074, Tfu_2176, and Tfu_0901, encoding endo-1,4-beta-glucanases
-
-
Manually annotated by BRENDA team
endoglucanase E-4 precursor; Cel9A
SwissProt
Manually annotated by BRENDA team
endoglucanase E1, E2, and E5
-
-
Manually annotated by BRENDA team
the bacterium produces six structurally and functionally distinct cellulases: Cel9B, Cel6A, Cel6B, Cel9A, Cel5A, Cel48A
-
-
Manually annotated by BRENDA team
Thermobifida fusca Cel6A
Cel6A
-
-
Manually annotated by BRENDA team
Thermobifida fusca Cel9A
endoglucanase E-4 precursor; Cel9A
SwissProt
Manually annotated by BRENDA team
most efficient producer of cellulase
-
-
Manually annotated by BRENDA team
Trichoderma sp.
-
-
-
Manually annotated by BRENDA team
Trichoderma sp.
strain C-4
-
-
Manually annotated by BRENDA team
strain C-4
-
-
Manually annotated by BRENDA team
sandfly, Phlebotomus papatasi, infected
-
-
Manually annotated by BRENDA team
from rumen of Bos mutus, Rucel5B
UniProt
Manually annotated by BRENDA team
in rabbit caecum
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
-
Trichoderma reesei produces a complete set of cellulase enzymes, comprising two cellobiohydrolases, i.e. 1,4-beta-D-glucan cellobiohydrolase, endoglucanases, i.e. 1,4- beta-D-glucan-4-glucanohydrolase, and beta-glucosidase, i.e. 1,4-beta-D-glucosidase, which act synergistically to degrade crystalline cellulose to glucose
metabolism
-
the mechanism for enzymatic hydrolysis of cellulose involves synergistic actions by endoglucanase, EC 3.2.1.4, exoglucanase or cellobiohydrolase, EC 3.2.1.91, and beta-glucosidase, EC 3.2.1.21
metabolism
-
the complete solubilization of crystalline cellulose requires the concerted and synergistic action of three classes of glycoside hydrolases, cellulase, endoglucanase, EC 3.2.1.4, cellobiohydrolase, EC 3.2.1.74, and beta-glucosidase, EC 3.2.1.21
metabolism
Brevibacillus sp.
-
three kinds of cellulases are required to carry out cellulose hydrolysis: endoglucanases, EC 3.2.1.4, exoglucanases or cellobiohydrolases, EC 3.2.1.91, and beta-glucosidases, EC 3.2.1.21
metabolism
B7UAM4
cellulolytic enzymes, including endo-beta-1,4-glucanases, EC 3.2.1.4, exo-beta-1,4-glucanases, EC 3.2.1.91, and beta-glucosidases, EC 3.2.1.21, collectively known as cellulases, act in a synergistic manner to facilitate complete cleavage of the cellulose beta-1,4-glycosidic bonds to form glucose
metabolism
-
the single GH9 cellulase is essential for cellulose degradation by the organism despite the presence of several other cellulases
metabolism
Bacillus subtilis I15
-
cellulolytic enzymes, including endo-beta-1,4-glucanases, EC 3.2.1.4, exo-beta-1,4-glucanases, EC 3.2.1.91, and beta-glucosidases, EC 3.2.1.21, collectively known as cellulases, act in a synergistic manner to facilitate complete cleavage of the cellulose beta-1,4-glycosidic bonds to form glucose
-
physiological function
O76130, -
for cellulose degradation, cellulase is an important enzyme for the first degradation step from cellulose to cellobiose
physiological function
-
cellulase 12A degrades cellulose molecules into smaller fragments, facilitating further utilization of the carbohydrate
physiological function
-
the enzyme is responsible for the main cellulase activity of Sulfolobus solfataricus
metabolism
-
three kinds of cellulases are required to carry out cellulose hydrolysis: endoglucanases, EC 3.2.1.4, exoglucanases or cellobiohydrolases, EC 3.2.1.91, and beta-glucosidases, EC 3.2.1.21
-
additional information
-
effects of different solvents, ions, and viscosities on enzyme activity at different temperatures, overview
additional information
F6M085
EBI-244 is a multidomain cellulase from a hyperthermophilic archaeon
additional information
-
mechanochemical model simulations and molecular dynamics of Cel9A, overview. Analysis of the electrostatic surface of the enzyme, overview
additional information
Desulfurococcaceae EBI-244
-
EBI-244 is a multidomain cellulase from a hyperthermophilic archaeon
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 cellobiose
cellotetraose + H2O
show the reaction diagram
-
transfer reaction
-
?
2 cellobiose
cellotetraose + H2O
show the reaction diagram
-
transfer reaction
-
?
2',4'-dinitrophenyl-beta-D-cellobiose + H2O
?
show the reaction diagram
-
-
-
-
?
2,3-di-O-methylated cellulase + H2O
?
show the reaction diagram
-
2,3-di-O-methylated cellulase, having a trace amount of unsubstituted glucose units is hydrolyzed. Only the linkages between glucose units and 2,3-di-O-methylated units are cleaved. No hydrolysis of 2,3-di-O-methylcellulose, having every structural unit of them regioselectively substituted
-
-
?
2,4-dinitrophenyl beta-D-cellobioside + H2O
?
show the reaction diagram
Thermobifida fusca, Thermobifida fusca Cel9A
P26221
-
-
-
?
4-methylumbelliferyl beta-cellotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl cellobioside + H2O
?
show the reaction diagram
Bacillus cellulyticus K-12
-
-
-
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + D-lactose
show the reaction diagram
Q8J0K6, Q8J0K7, Q8J0K8
very low activity
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
low activity
-
-
?
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
Pyrococcus horikoshii DSM 12428
O58925
-
-
-
?
4-nitrophenyl cellobioside + H2O
4-nitrophenol + cellobiose
show the reaction diagram
Pyrococcus horikoshii DSM 12428
-
-
-
-
?
4-nitrophenyl cellobioside + H2O
?
show the reaction diagram
Desulfurococcaceae, Desulfurococcaceae EBI-244
F6M085
-
-
-
?
4-nitrophenyl cellopentaoside + H2O
4-nitrophenol + cellopentaose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl cellotetraoside + H2O
4-nitrophenol + cellotetraose
show the reaction diagram
-
-
-
-
?
acid swollen cellulose + H2O
cellobiose + cellotriose
show the reaction diagram
Q9PF60, -
endoglucanase with exohydrolytic activity
-
-
?
acid-swollen cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
acid-swollen cellulose + H2O
?
show the reaction diagram
Q3LHN3
-
-
-
?
acid-swollen cellulose + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
acid-swollen cellulose + H2O
?
show the reaction diagram
Q9LCC5
activity of truncated enzyme form
-
-
?
acid-swollen cellulose + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
cleaved cellulose randomly
-
-
?
acid-swollen cellulose + H2O
?
show the reaction diagram
Ruminococcus albus F-40
Q9LCC5
activity of truncated enzyme form
-
-
?
acid-swollen cellulose + H2O
?
show the reaction diagram
Clostridium thermocellum CelJ
-
-
-
-
?
alpha-cellulose + H2O
?
show the reaction diagram
-
activity of the catalytic module EG1-CM is 58% of the activity with endoglucanase 1
-
-
?
alpha-chitin + H2O
?
show the reaction diagram
-
due to the binding, it seems chitin would be an inhibitor of Cel6B, Cel9A and Cel48A, but not of Cel6A and Cel5A
-
-
?
amorphous cellulose + H2O
?
show the reaction diagram
Q6BCL3, Q6BCL7, Q6BCM1
-
-
-
?
arabinan + H2O
?
show the reaction diagram
Q97YG7, -
-
-
-
?
arabinan + H2O
?
show the reaction diagram
Q97YG7, -
hydrolysed at 53% compared to the hydrolysis of carboxymethylcellulose
-
-
?
avicel + H2O
?
show the reaction diagram
Q9STD9
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
avicel + H2O
?
show the reaction diagram
Q8J0K6, Q8J0K7, Q8J0K8
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
glucose + cellobiose + cellotriose + cellotetraose
?
avicel + H2O
?
show the reaction diagram
Bacillus cellulyticus K-12
-
-
cellobiose + cellotetraose
?
avicel + H2O
?
show the reaction diagram
-
-
main product is cellobiose
?
avicel + H2O
?
show the reaction diagram
-
weak activity
-
-
?
avicel + H2O
?
show the reaction diagram
-
weak activity
-
-
?
avicel + H2O
?
show the reaction diagram
Q46002
weak activity
-
-
?
avicel + H2O
?
show the reaction diagram
-
low activity
-
-
?
avicel + H2O
?
show the reaction diagram
-
no hydrolysis
-
-
-
avicel + H2O
?
show the reaction diagram
-
no hydrolysis
-
-
-
avicel + H2O
?
show the reaction diagram
-
cellulase I
-
-
?
avicel + H2O
?
show the reaction diagram
-
chimeric xylanase/endoglucanase
-
-
?
avicel + H2O
?
show the reaction diagram
-
endoglucanase I show very low activity
-
-
?
avicel + H2O
?
show the reaction diagram
-
14% of the activity with carboxymethylcellulose
-
-
?
avicel + H2O
?
show the reaction diagram
-
33% of the activity with carboxymethyl cellulose
-
-
?
avicel + H2O
?
show the reaction diagram
-
activity of the catalytic module EG1-CM is43% of the activity with endoglucanase 1
-
-
?
avicel + H2O
?
show the reaction diagram
-
endoglucanase 47
-
-
?
avicel + H2O
?
show the reaction diagram
-
microcrystalline cellulose (Avicel) is subjected to 3 different pretreatments (acid, alkaline, and organic solvent) before exposure to amixture of cellulases
-
-
?
avicel + H2O
?
show the reaction diagram
-
microcrystalline cellulose, shows poor growth and enzyme production, relative activity 5.2%
-
-
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
Clostridium thermocellum CelJ
-
weak activity
-
-
?
avicel + H2O
?
show the reaction diagram
Bacillus agaradhaerens JAM-KU023
-
33% of the activity with carboxymethyl cellulose
-
-
?
avicel + H2O
?
show the reaction diagram
Bacillus sp. CTP-09
-
microcrystalline cellulose, shows poor growth and enzyme production, relative activity 5.2%
-
-
?
avicel + H2O
?
show the reaction diagram
Pyrococcus horikoshii DSM 12428
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
Bacillus sp. HR68
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
Bacillus amyloliquefaciens DL-3
-
14% of the activity with carboxymethylcellulose
-
-
?
avicel + H2O
cellobiose + ?
show the reaction diagram
Pyrococcus horikoshii, Pyrococcus horikoshii DSM 12428
-
the main product is cellobiose
-
-
?
avicel + H2O
cellobiose + cellotriose
show the reaction diagram
Q9PF60, -
cellooligosaccharides production from Avicel is detected only when 0.00185 mkat of Xf818his are employed, and not when 16.67 nkat are used. Endoglucanase with exohydrolytic activity
-
-
?
avicel + H2O
cellobiose + glucose
show the reaction diagram
-
the main product is cellobiose together with a small amount of glucose, endoglucanase 35
-
-
?
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
P26221
-
-
-
?
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
Q46002
weak activity
-
-
?
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
Thermobifida fusca Cel9A
P26221
-
-
-
?
bacterial microcrystalline cellulose + H2O
cellotetraose
show the reaction diagram
-
weak activity
83% cellotetraose + 11% cellotriose + 4% cellobiose
-
?
bacterial micropcrystalline cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
ball-milled cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
barley beta-D-glucan + H2O
?
show the reaction diagram
-
-
-
-
?
barley beta-D-glucan + H2O
?
show the reaction diagram
Q97YG7
-
-
-
?
barley beta-glucan + H2O
?
show the reaction diagram
-
-
-
-
?
barley beta-glucan + H2O
?
show the reaction diagram
-
soluble substrate
-
-
?
barley beta-glucan + H2O
?
show the reaction diagram
-
the highest specific activity toward polysaccharides occurrs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-glucan and lichenan
-
-
?
barley beta-glucan + H2O
?
show the reaction diagram
Bacillus subtilis JA18
-
soluble substrate
-
-
?
barley glucan + H2O
?
show the reaction diagram
-
-
-
-
?
barley glucan + H2O
?
show the reaction diagram
Q9X273
the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose
-
-
?
barley glucan + H2O
?
show the reaction diagram
Bacillus subtilis BME-15
-
-
-
-
?
beechwood xylan + H2O
?
show the reaction diagram
Q97YG7, -
hydrolysed at 84% compared to the hydrolysis of carboxymethylcellulose
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
-
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
-
-
-
?
beta-glucan + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
endoglucanase EG25, endoglucanase EG28, endoglucanase EG44
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
endoglucanase EG47, endoglucanase EG51, endoglucanase EG60
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
24.6% of the activity with carboxymethylcellulose
-
-
?
beta-glucan + H2O
?
show the reaction diagram
D2DRB6
from Hordeum vulgare
-
-
?
beta-glucan + H2O
?
show the reaction diagram
Bacillus sp. CH43, Bacillus sp. HR68
-
-
-
-
?
beta-glucan + H2O
?
show the reaction diagram
Bacillus amyloliquefaciens DL-3
-
24.6% of the activity with carboxymethylcellulose
-
-
?
birchwood xylan + H2O
?
show the reaction diagram
Q97YG7, -
hydrolysed at 73% compared to the hydrolysis of carboxymethylcellulose
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Geobacillus sp.
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
P26221
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q3LHN3
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q75UV6
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q6BCL3, Q6BCL7, Q6BCM1
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-, Q9BLD2
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q83XK5
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
A4UU22
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
B5BNY1, -
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
G3BJX5
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
F6M085
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
no glucose, cellobiose and short chain celloolifgosaccharides are formed
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
68% of the activity with barley beta-glucan
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q9LCC5
activity of truncated enzyme form
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
endo-beta-1,4-glucanase EG27 shows high preference for, endo-beta-1,4-glucanase EG45 shows high preference for
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
endoglucanase EG25, endoglucanase EG28, endoglucanase EG44
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
endoglucanase EG47, endoglucanase EG51, endoglucanase EG60
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
Cel9A is a nonprocessive enzyme with endo-cellulase activities
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
endoglucanase 35, endoglucanase 47
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q9PF60, -
endoglucanase with exohydrolytic activity
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q3ZMA8
processive endoglucanase activity
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Ruminococcus albus F-40
Q9LCC5
activity of truncated enzyme form
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Bacillus agaradhaerens JAM-KU023
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Mucor circinelloides NRRL 26519
-
-
no glucose, cellobiose and short chain celloolifgosaccharides are formed
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Cellulomonas flavigena 531
Q3ZMA8
processive endoglucanase activity
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Desulfurococcaceae EBI-244
F6M085
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Pyrococcus horikoshii DSM 12428
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Bacillus sp. HSH-810
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Rhizobium leguminosarum bv. trifolii ANU843
Q83XK5
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Thermobifida fusca Cel9A
P26221
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Acremonium cellulolyticus CF-2612
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Bacillus sp. DUSELR13
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q97YG7
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Geobacillus sp. WSUCFI
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Acidothermus cellulolyticus E1
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Thermobifida fusca Cel6A
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Staphylotrichum coccosporum NBRC 31817
B5BNY1
-
-
-
?
carboxymethyl cellulose + H2O
cellobiose + ?
show the reaction diagram
Q25C15
-
-
-
?
carboxymethyl cellulose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
D-glucose + cellobiose + cellooligosaccharide
show the reaction diagram
Cherax destructor, Cherax destructor Clark
-
-
-
-
?
carboxymethyl-cellulose + H2O
?
show the reaction diagram
Q97YG7, -
-
-
-
?
carboxymethyl-cellulose + H2O
?
show the reaction diagram
Bacillus subtilis, Bacillus subtilis I15
B7UAM4
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
A7UGD9
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Q46002
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Q8J0K6, Q8J0K7, Q8J0K8
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
D2DRB6
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Q9STD9
activity with carbomethylcellulose with approxomately 4 carboxymethyl groups per 10 anhydro-glucose units is about 3.5 times better than that with carbomethylcellulose with approximately 8 carboxymethyl groups
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Q9X273
the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
the smallest product is a disaccharide
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
soluble substrate
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
enzyme is specific for carboxymethylcellulose
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
A7HLZ2, -
high specific activity towards carboxymethylcellulose
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
medium-viscosity substrate
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Clostridium thermocellum CelJ
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Bacillus sp. CTP-09
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Bacillus subtilis AS3
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Aspergillus oryzae cmc-1
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Cellulomonas biazotea NIAB 442
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Alicyclobacillus acidocaldarius ATCC27009
-
enzyme is specific for carboxymethylcellulose
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Bacillus sp. HR68
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Bacillus subtilis JA18
-
soluble substrate
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Bacillus circulans KSM-N257
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Chaetomium thermophilum CT2
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Bacillus amyloliquefaciens DL-3
-
-
-
-
?
carboxymethylcellulose + H2O
carboxymethyl glucose + cellobiose + cellotriose
show the reaction diagram
Lysinibacillus sphaericus, Lysinibacillus sphaericus JS1
-
-
-
-
?
carboxymethylcellulose + H2O
cellobiose + ?
show the reaction diagram
Q97X08, -
-
-
-
?
carboxymethylcellulose + H2O
cellobiose + ?
show the reaction diagram
Q97X08, -
-
-
-
?
carboxymethylcellulose + H2O
cellobiose + cello-oligomer
show the reaction diagram
Q97YG7, -
-
-
-
?
carboxymethylcellulose + H2O
short glucose oligomers + cellobiose + glucose
show the reaction diagram
Gecarcoidea natalis, Gecarcoidea natalis Pocock 1888
-
-
-
-
?
carboxymethylcellulose + H2O
cellobiose + cellooligomers
show the reaction diagram
Q97YG7, -
endo-beta-1,4-glucanase activity. The enzyme shows cellulase and xylanase activity
-
-
?
carboxymethylpachyman + H2O
?
show the reaction diagram
-
cellulase I
-
-
?
cello-oligosaccharide + H2O
?
show the reaction diagram
-
insoluble cello-oligosaccharide average degree of polymerization 34 and soluble cello-oligosaccharides longer than cellotriose
-
-
?
cellobiose + H2O
?
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
?
show the reaction diagram
-, Q9BLD2
-
-
-
?
cellobiose + H2O
?
show the reaction diagram
A4UU22
-
-
-
?
cellobiose + H2O
?
show the reaction diagram
-
8.6% of the activity with carboxymethylcellulose
-
-
?
cellobioside + H2O
?
show the reaction diagram
-
about 2% of the activity with barley beta-glucan
-
-
?
cellodextrin + H2O
?
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
-
?
cellodextrin + H2O
?
show the reaction diagram
-
cellulase A, B, and C
-
-
?
celloheptaose + H2O
?
show the reaction diagram
Robillarda sp., Robillarda sp. Y-20
-
-
-
-
?
cellohexaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
cellohexaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
cellotriose
show the reaction diagram
Bacillus circulans, Bacillus circulans KSM-N257
-
-
+ small amounts of cellobiose and cellotriose
-
?
cellohexaose + H2O
cellobiose + cellotriose + cellotetraose
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
-
?
cellohexaose + H2O
cellotriose + cellotetraose + cellobiose
show the reaction diagram
Q25C15
major product is cellotriose, minor products are cellobiose and cellotetraose
-
-
?
cellooligosaccharide + H2O
?
show the reaction diagram
-
-
-
-
?
cellooligosaccharide + H2O
?
show the reaction diagram
-
insolvent cellooligosaccharide 33
-
-
?
cellooligosaccharides + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
random cleavage, slow reaction rates for cellooligosaccharides smaller than G5
-
-
?
cellopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
Q9PF60, -
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
cellobiose + cellotriose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
cellobiose + cellotriose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
cellobiose + cellotriose
show the reaction diagram
Bacillus circulans KSM-N257
-
-
-
-
?
cellopentaose + H2O
cellotetraose + glucose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
cellobiose + glucose
show the reaction diagram
-
-
-
-
?
cellopentaosyl sorbitol + H2O
?
show the reaction diagram
-
cellulase A, B, and C
-
-
?
cellopentose + H2O
cellobiose + cellotriose
show the reaction diagram
Q97X08, -
-
-
-
?
cellopentose + H2O
cellobiose + cellotriose
show the reaction diagram
Q97X08, -
-
-
-
?
cellotetraose
cellopentaose + cellohexaose
show the reaction diagram
Robillarda sp., Robillarda sp. Y-20
-
transfer reaction
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
Robillarda sp.
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
Robillarda sp.
-
-
predominant product
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
+ glucose + cellotriose
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
Gluconacetobacter xylinus
-
-
+ glucose + cellotriose
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
+ glucose + cellotriose
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
+ glucose + cellotriose
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
glucose, 15%, cellobiose, 64%, cellotriose, 14% and cellotetraose, 3.3%
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
enzyme form EGB and EGC
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
cellulase A, B, and C
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
Bacillus sp. N-4
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
predominant product
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
Ruminococcus albus F40, Bacillus sp. No. 1139
-
-
-
-
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
Bacillus sp. No. 1139
-
-
glucose, 15%, cellobiose, 64%, cellotriose, 14% and cellotetraose, 3.3%
?
cellotetraose + H2O
2 cellobiose
show the reaction diagram
Bacillus circulans F-2
-
-
-
?
cellotetraose + H2O
?
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
?
show the reaction diagram
Q9PF60, -
-
-
-
?
cellotetraose + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
cellotetraose + H2O
?
show the reaction diagram
-, Q9BLD2
-
-
-
?
cellotetraose + H2O
cellobiose
show the reaction diagram
Q97X08, -
minimal substrate length, cellotriose and cellobiose are not hydrolyzed
-
-
?
cellotetraose + H2O
cellobiose
show the reaction diagram
Q97X08, -
minimal substrate length, cellotriose and cellobiose are not hydrolyzed
-
-
?
cellotetraosyl sorbitol + H2O
?
show the reaction diagram
-
cellulase A, B, and C
-
-
?
cellotriose
cellopentaose + cellotetraose
show the reaction diagram
Robillarda sp., Robillarda sp. Y-20
-
transfer reaction
-
?
cellotriose + H2O
?
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
?
show the reaction diagram
-, Q9BLD2
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
slightly degraded
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
-
-
-
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
Robillarda sp.
-
-
predominant product
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
a mixture of glucose, 5.5%, cellobiose, 55%, cellotriose, 34%, and traces of cellotetraose
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
+ glucose
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
+ glucose
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
no activity
-
-
-
cellotriose + H2O
cellobiose
show the reaction diagram
-
no reaction
-
-
-
cellotriose + H2O
cellobiose
show the reaction diagram
-
no reaction
-
-
-
cellotriose + H2O
cellobiose
show the reaction diagram
-
no hydrolysis
-
-
-
cellotriose + H2O
cellobiose
show the reaction diagram
-
enzyme form EGC
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
effectively hydrolyzed by endoglucanase E1, limited hydrolysis by endoglucanase E2
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
hydrolyzed by endoglucanase E1, no activity with endoglucanase E2
+ glucose, endoglucanase E1
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
cellulase C
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
negligibly hydrolyzed
-
-
-
cellotriose + H2O
cellobiose
show the reaction diagram
-
hydrolyzed by endo-beta-1,4-glucanase component YEG2, no hydrolysis with endo-beta-1,4-glucanase component YEG1
+ glucose
?
cellotriose + H2O
cellobiose
show the reaction diagram
Bacillus sp. N-4
-
-
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
-
-
predominant product
?
cellotriose + H2O
cellobiose
show the reaction diagram
Bacillus sp. No. 1139
-
-
-
-
?
cellotriose + H2O
cellobiose
show the reaction diagram
Bacillus sp. No. 1139
-
-
a mixture of glucose, 5.5%, cellobiose, 55%, cellotriose, 34%, and traces of cellotetraose
?
cellotriose + H2O
cellobiose
show the reaction diagram
Bacillus circulans F-2
-
no reaction
-
-
-
cellotrioside + H2O
?
show the reaction diagram
-
7.4% of the activity with barley beta-glucan
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Geobacillus sp.
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
O00095
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
A0ZT16, -
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Q97YG7, -
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Q8J0K6, Q8J0K7, Q8J0K8
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Q40763
-
-
-
?
cellulose + H2O
?
show the reaction diagram
C5I945, C5I946
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
crystalline cellulose is hydrolyzed to glucose and cellobiose
?
cellulose + H2O
?
show the reaction diagram
Athelia rolfsii, Penicillium funiculosum
-
Walseth cellulose
-
-
?
cellulose + H2O
?
show the reaction diagram
-
Walseth cellulose
products from Walseth cellulose: glucose + cellobiose + cellotriose + cellotetraose
?
cellulose + H2O
?
show the reaction diagram
Penicillium funiculosum
-
Walseth cellulose
mainly glucose, cellobiose and traces of cellotriose
?
cellulose + H2O
?
show the reaction diagram
-
endoglucanase E1 exhibits higher activity
-
?
cellulose + H2O
?
show the reaction diagram
-
endoglucanase I shows very low activity with acid-swollen cellulose
-
-
-
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
Myceliophthora heterothallica, Penicillium funiculosum
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
Sporotrichum pulverulentum
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
large amounts of cellotriose and a small amount of cellotetraose
?
cellulose + H2O
?
show the reaction diagram
-
acid-swollen
major end product are cellobiose and cellotriose, endoglucanase Z
?
cellulose + H2O
?
show the reaction diagram
-
swollen cellulose
a mixture of cellooligosaccharides
?
cellulose + H2O
?
show the reaction diagram
-
no degradation of crystalline cellulose
-
-
-
cellulose + H2O
?
show the reaction diagram
-
Walseth cellulose shows 1.1% of the activity with carboxymethylcellulose
-
-
?
cellulose + H2O
?
show the reaction diagram
-
amorphous cellulose and ball-milled cellulose
-
-
?
cellulose + H2O
?
show the reaction diagram
-
little ability to hydrolyze ordered cellulose
-
-
-
cellulose + H2O
?
show the reaction diagram
-
enzyme form EGA and EGD
-
-
?
cellulose + H2O
?
show the reaction diagram
-
amorphous cellulose
-
-
?
cellulose + H2O
?
show the reaction diagram
-
amorphous cellulose
endoglucanase E1 produces mainly cellobiose and a trace of glucose, endoglucanase E2 produces mainly cellotriose and higher oligomers with some cellobiose and a trace of glucose
?
cellulose + H2O
?
show the reaction diagram
-
partial degradation of native cellulose
-
-
-
cellulose + H2O
?
show the reaction diagram
-
no hydrolysis of native cellulose
-
-
-
cellulose + H2O
?
show the reaction diagram
-
amorphous cellulose (relative activity 15.2%), filter paper (shows poor growth and enzyme production, relative activity 3.1%), cotton wool (relative activity 4.3%)
-
-
?
cellulose + H2O
?
show the reaction diagram
-
Avicel or coastal Bermuda grass
-
-
?
cellulose + H2O
?
show the reaction diagram
Q8J0K6, Q8J0K7, Q8J0K8
crystalline and amorphous cellulose
-
-
?
cellulose + H2O
?
show the reaction diagram
-
the catalytic domains of Cel6A, Cel6B, Cel48A, Cel5A, and Cel9A only show very weak binding to bacterial cellulose
-
-
?
cellulose + H2O
?
show the reaction diagram
-
hydrolysis of Solka floc, Avicel, CMC, waste paper, and waste wood refiner
-
-
?
cellulose + H2O
?
show the reaction diagram
-
the family 1 carbohydrate-binding module, CBM, mediates the interaction between enzyme and crystalline cellulose surface via residues Y5, Q7, N29, and Y32, thus CBM is responsible for anchoring the enzyme at discrete points along a cellulose chain to aid in both recognizing cellulose chain ends for initial attachment to cellulose as well as aid in enzymatic catalysis by diffusing between stable wells on a length scale commensurate with the catalytic, processive cycle of Cel7A during cellulose hydrolysis, molecular-level mechanisms of recognition and interaction, overview
-
-
?
cellulose + H2O
?
show the reaction diagram
-
activity is evaluated on 13 insoluble celluloses characterized for crystallinity and crystal width (by X-ray diffraction), wet porosity (by thermoporometry), and particle size (by light scattering). Looser crystalline packing increases the lengths of released cello-oligomers as well as the maximum endoglucanase specific activity (kcat)
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Bacillus sp. CTP-09
-
amorphous cellulose (relative activity 15.2%), filter paper (shows poor growth and enzyme production, relative activity 3.1%), cotton wool (relative activity 4.3%)
-
-
?
cellulose + H2O
?
show the reaction diagram
Pyrococcus horikoshii DSM 12428
O58925
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Aspergillus niger IFO31125
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
Talaromyces emersonii CBS 814.70
-
acid-swollen
-
-
?
cellulose + H2O
?
show the reaction diagram
Streptomyces rochei A2
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Ruminococcus albus F40
-
acid-swollen
-
?
cellulose + H2O
?
show the reaction diagram
Acremonium cellulolyticus CF-2612
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Marinobacter sp. MSI032
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Bacillus sp. DUSELR13
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Fibrobacter succinogenes S85
-
swollen cellulose
a mixture of cellooligosaccharides
?
cellulose + H2O
?
show the reaction diagram
Bacillus subtilis JA18
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Geobacillus sp. WSUCFI
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Bacillus subtilis LN
C5I945, C5I946
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Bacillus subtilis BME-15
-
-
-
-
?
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
-
-
-
?
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
inducible enzyme
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
induced by carboxymethylcellulose, non-constitutive synthesis
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
Sporotrichum pulverulentum
-
enzyme is involved in degradation of cellulose
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
enzyme plays an important role in the carbon cycle
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
in bean the 9.5 cellulase appears to function in the shedding of various organs such as fruits, flowers, and leaves. The acid cellulases function in loosening the cellulose fibrils of the cell wall to allow expansion and growth
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
the enzyme plays an important role in maintaining the carbon balance in nature
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
Myceliophthora heterothallica D-14
-
enzyme plays an important role in the carbon cycle
-
-
-
cellulose + H2O
cellobiose
show the reaction diagram
O76130, -
-
-
-
?
cellulose + H2O
cellobiose + ?
show the reaction diagram
Q97X08, -
the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source
-
-
?
cellulose + H2O
cellobiose + ?
show the reaction diagram
Q97X08, -
the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source
-
-
?
cellulose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
D-glucose + ?
show the reaction diagram
A7HLZ2, -
-
-
-
?
cellulose + H2O
D-glucose + ?
show the reaction diagram
Q97YG7, -
at 90C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases
-
-
?
cellulose + H2O
cellobiose + glucose
show the reaction diagram
-
-
-
-
?
chitotetraose + H2O
?
show the reaction diagram
-
-
-
-
?
chitotriose + H2O
?
show the reaction diagram
-
-
-
-
?
CM cellulose + H2O
?
show the reaction diagram
A4UU22
-
-
-
?
crystalline cellulase + H2O
?
show the reaction diagram
-
-
-
-
?
crystalline cellulose
?
show the reaction diagram
-
-
-
-
?
crystalline cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
crystalline cellulose + H2O
?
show the reaction diagram
-
as natural substrate for CEL9C1
-
-
?
curdlan + H2O
?
show the reaction diagram
Q97YG7, -
activity is 26% compared to activity with carboxymethyl-cellulose
-
-
?
debranched arabinan
?
show the reaction diagram
Q97YG7, -
activity is 25% compared to activity with carboxymethyl-cellulose
-
-
-
dextran + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
glucan + H2O
?
show the reaction diagram
-
barley beta-glucan
-
-
?
glucan + H2O
?
show the reaction diagram
-
barley beta-glucan
-
-
?
glucan + H2O
?
show the reaction diagram
-
yeast glucan
-
-
?
glucan + H2O
?
show the reaction diagram
-
catalytic domain of endoglucanase G
-
-
?
glucan + H2O
?
show the reaction diagram
Fibrobacter succinogenes S85
-
barley beta-glucan
-
-
?
glucan + H2O
?
show the reaction diagram
Fibrobacter succinogenes S85
-
catalytic domain of endoglucanase G
-
-
?
glucan + H2O
?
show the reaction diagram
Fibrobacter succinogenes S85
-
barley beta-glucan
-
-
?
glucomannan + H2O
?
show the reaction diagram
Q6BCL3, Q6BCL7, Q6BCM1
-
-
-
?
hydroxyethyl cellulose + H2O
?
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
-
?
hydroxyethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
hydroxyethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
hydroxyethylcellulose + H2O
?
show the reaction diagram
-
-
-
-
?
hydroxyethylcellulose + H2O
?
show the reaction diagram
Q8J0K6, Q8J0K7, Q8J0K8
-
-
-
?
insoluble cellulose + H2O
?
show the reaction diagram
Mucor circinelloides, Mucor circinelloides NRRL 26519
-
e.g. Avicel, Solka-floc, Sigmacell 50
no glucose, cellobiose and short chain celloolifgosaccharides are formed
-
?
insoluble H3PO4 acid-swollen cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
cellulase I
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Q3LHN3
-
-
-
?
lichenan + H2O
?
show the reaction diagram
O65987, Q9AQF4, Q9LAJ2, Q9RGE6
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Q6BCL3, Q6BCL7, Q6BCM1
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
D2DRB6
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Q46002
weak activity
-
-
?
lichenan + H2O
?
show the reaction diagram
-
6% of the activity with carboxymethylcellulose
-
-
?
lichenan + H2O
?
show the reaction diagram
-
enzyme form EGD
-
-
?
lichenan + H2O
?
show the reaction diagram
-
30% of the activity with barley beta-glucan, catalytic domain of endoglucanase G
-
-
?
lichenan + H2O
?
show the reaction diagram
-
endoglucanase A
-
-
?
lichenan + H2O
?
show the reaction diagram
-
cellulase I, II and III
-
-
?
lichenan + H2O
?
show the reaction diagram
-
9% of the activity with barley beta-glucan
-
-
?
lichenan + H2O
?
show the reaction diagram
Q9LCC5
activity of truncated enzyme form
-
-
?
lichenan + H2O
?
show the reaction diagram
Q9X273
the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose
-
-
?
lichenan + H2O
?
show the reaction diagram
-
the smallest product is a disaccharide
-
-
?
lichenan + H2O
?
show the reaction diagram
-
380% of the activity with carboxymethyl cellulose
-
-
?
lichenan + H2O
?
show the reaction diagram
Q97YG7, -
activity is 120% compared to activity with carboxymethyl-cellulose
-
-
?
lichenan + H2O
?
show the reaction diagram
-
the highest specific activity toward polysaccharides occurrs with mixed-linkage (1->3),(1->4)-beta-D-glucans such as barley beta-D-glucan and lichenan
-
-
?
lichenan + H2O
?
show the reaction diagram
Ruminococcus albus F-40
Q9LCC5
activity of truncated enzyme form
-
-
?
lichenan + H2O
?
show the reaction diagram
Clostridium thermocellum CelJ
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Bacillus agaradhaerens JAM-KU023
-
380% of the activity with carboxymethyl cellulose
-
-
?
lichenan + H2O
?
show the reaction diagram
Pyrococcus horikoshii DSM 12428
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Fibrobacter succinogenes S85
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Fibrobacter succinogenes S85
-
30% of the activity with barley beta-glucan, catalytic domain of endoglucanase G
-
-
?
lichenan + H2O
?
show the reaction diagram
Q97YG7
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Bacillus circulans KSM-N257
-
-
-
-
?
lichenin + H2O
?
show the reaction diagram
-
-
-
-
?
lignocellulose + H2O
?
show the reaction diagram
-
-
-
-
?
lignocellulose + H2O
?
show the reaction diagram
-
solubilizes lignocellulose
-
-
?
mannan + H2O
?
show the reaction diagram
-
-
-
-
?
methylcellulose + H2O
?
show the reaction diagram
Talaromyces emersonii, Talaromyces emersonii CBS 814.70
-
-
-
-
?
N,N',N'',N''', N''''-triacetyl-chitopentaose + H2O
N,N'-diacetyl-chitobiose + N,N',N''-triacetyl-chitotriose
show the reaction diagram
-
the enzyme liberates disaccharides from the reducing end
-
-
?
N,N',N''-triacetyl-chitotriose + H2O
N,N'-diacetyl-chitobiose + N-acetyl-D-glucosamine
show the reaction diagram
-
the enzyme liberates disaccharides from the reducing end
-
-
?
N-acetyllactosamine + butanol
butyl beta-N-acetyllactosaminide + H2O
show the reaction diagram
-
-
-
-
?
N-acetyllactosamine + ethanol
ethyl beta-N-acetyllactosaminide + H2O
show the reaction diagram
-
-
-
-
?
N-acetyllactosamine + glycerol
glyceroyl beta-N-acetyllactosaminide
show the reaction diagram
-
-
-
-
?
N-acetyllactosamine + propanol
proyl beta-N-acetyllactosaminide + H2O
show the reaction diagram
-
-
-
-
?
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O
?
show the reaction diagram
-
-
-
-
?
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate + H2O
?
show the reaction diagram
Q97X08, -
-
-
-
?
noncrystalline cellulose + H2O
?
show the reaction diagram
-
as natural substrate for CEL7
-
-
?
o-nitrophenyl beta-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl beta-cellobioside + H2O
?
show the reaction diagram
O00095
-
-
-
?
o-nitrophenyl beta-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
oat spelt xylan + H2O
?
show the reaction diagram
Q3LHN3
-
-
-
?
oat spelt xylan + H2O
?
show the reaction diagram
Q97YG7, -
activity is 25% compared to activity with carboxymethyl-cellulose
-
-
?
oat spelt xylan + H2O
?
show the reaction diagram
Q97YG7, -
hydrolysed at 76% compared to the hydrolysis of carboxymethylcellulose
-
-
?
p-nitrophenyl beta-D-cellobioside + H2O
glucose + cellobiose + ?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl beta-D-cellobioside + H2O
glucose + cellobiose + ?
show the reaction diagram
-
-
-
?
p-nitrophenyl beta-D-cellobioside + H2O
glucose + cellobiose + ?
show the reaction diagram
Bacillus cellulyticus K-12
-
-
-
-
?
p-nitrophenyl beta-D-cellobioside + H2O
glucose + cellobiose + ?
show the reaction diagram
-
cellulase III preferentially attacks the aglycone linkage
-
-
?
p-nitrophenyl beta-D-cellobioside + H2O
glucose + cellobiose + ?
show the reaction diagram
-
cellulase II-A preferentially attacks the holoside linkage of p-nitrophenyl beta-D-cellobioside, cellulase II-B attacks mainly the aglycone linkage. Synthesis of cellotriose from p-nitrophenyl beta-D-cellobioside by transfer of a glucosyl residue, possibly to cellobiose produced in the reaction mixture
-
-
?
p-nitrophenyl cellobiose + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl cellobiose + H2O
p-nitrophenol + cellobiose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
?
show the reaction diagram
-
endoglucanase EG25, endoglucanase EG28, endoglucanase EG44
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
?
show the reaction diagram
-
endoglucanase EG47, endoglucanase EG51, endoglucanase EG60
-
-
?
p-nitrophenyl-beta-D-cellopentaoside + H2O
?
show the reaction diagram
Q9X273
-
-
-
?
p-nitrophenyl-beta-D-cellotetraoside + H2O
?
show the reaction diagram
Q9X273
-
-
-
?
p-nitrophenyl-cellobiose + H2O
?
show the reaction diagram
Q46002
-
-
-
?
pachyman + H2O
?
show the reaction diagram
Q97YG7, -
activity is 22% compared to activity with carboxymethyl-cellulose
-
-
?
phosphoric acid swollen cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
phosphoric acid swollen cellulose + H2O
?
show the reaction diagram
Q8J0K6, Q8J0K7, Q8J0K8
-
-
-
?
phosphoric acid swollen cellulose + H2O
?
show the reaction diagram
-
fluorimetric determination in cellulase activity assay using calcofluor white
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
Q9STD9
-
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
Q46002
-
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
P26221
-
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
-
weak
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
-
low activity
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
Thermobifida fusca Cel9A
P26221
-
-
-
?
phosphoric acid-swollen cellulose + H2O
?
show the reaction diagram
Chaetomium thermophilum CT2
-
low activity
-
-
?
pullulan + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
remazol brilliant blue R-carboxymethylcellulose + H2O
?
show the reaction diagram
Alicyclobacillus acidocaldarius, Alicyclobacillus acidocaldarius ATCC27009
-
-
-
-
?
Sigmacell 101 + H2O
?
show the reaction diagram
-
low activity, endo-beta-1,4-glucanase EG27, low activity, endo-beta-1,4-glucanase EG45
-
-
?
sodium carboxymethyl cellulose + H2O
?
show the reaction diagram
Bacillus subtilis, Bacillus subtilis BME-15
-
-
-
-
?
soluble cellodextrin + H2O
?
show the reaction diagram
Mucor circinelloides, Mucor circinelloides NRRL 26519
-
-
no glucose, cellobiose and short chain cellooligosaccharides are formed
-
?
soluble cellulose + H2O
?
show the reaction diagram
-
endo mode of action. At the beginning only high molecular mass products are released suggesting an endowise action of the enzyme. The major product has a degree of polymerization of three, minor quantities of smaller and larger oligosaccharides are also produced. Not active against insoluble cellulose
-
-
?
xylan + H2O
?
show the reaction diagram
Q46002
weak activity
-
-
?
xylan + H2O
?
show the reaction diagram
-
about 2% of the activity with barley beta-glucan
-
-
?
xylan + H2O
?
show the reaction diagram
Q9LCC5
activity of truncated enzyme form
-
-
?
xylan + H2O
?
show the reaction diagram
-
the smallest product is a disaccharide
-
-
?
xylan + H2O
?
show the reaction diagram
-
low acrtivity, endo-beta-1,4-glucanase EG27, low acrtivity, endo-beta-1,4-glucanase EG45
-
-
?
xylan + H2O
?
show the reaction diagram
-
22.5% of the activity with carboxymethylcellulose
-
-
?
xylan + H2O
?
show the reaction diagram
Ruminococcus albus F-40
Q9LCC5
activity of truncated enzyme form
-
-
?
xylan + H2O
D-xylose + ?
show the reaction diagram
Q97YG7, -
at 90C, pH 4 by an enzyme mix consisting of SSO1354 and additional glycosyl hydrolases
-
-
?
xyloglucan + H2O
?
show the reaction diagram
-
-
-
-
?
xyloglucan + H2O
?
show the reaction diagram
Q40763
-
-
-
?
xyloglucan + H2O
?
show the reaction diagram
-
as natural substrate in CEL7
-
-
?
4-nitrophenyl cellotrioside + H2O
4-nitrophenol + cellotriose
show the reaction diagram
-
-
-
-
?
6-O-methylcellulose + H2O
additional information
-
-
6-O-methylcellulose having every structural unit of them regioselectively substituted
glycosidic bond between two adjacent substituted units can be cleaved to give oligomers with a degree of polymerization of ca. 8
?
carboxymethyl cellulose + H2O
glucose + cellobiose + short oligomers
show the reaction diagram
Gecarcoidea natalis, Gecarcoidea natalis Pocock 1888
-
-
-
-
?
carboxymethyl cellulose + H2O
additional information
-
Cherax destructor, Cherax destructor Clark
-
-
short glucose oligomers, cellobiose and a very small amount of glucose
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Bacillus subtilis BME-15
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Penicillium funiculosum
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Lenzites trabea
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Robillarda sp.
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Bacillus cellulyticus K-12
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
glucose + cellobiose + cellotriose + cellotetraose
?
carboxymethylcellulose + H2O
additional information
-
-
-
various cellooligosaccharides
?
carboxymethylcellulose + H2O
additional information
-
-
-
glucose + cellobiose
?
carboxymethylcellulose + H2O
additional information
-
Robillarda sp.
-
-
mainly glucose with small amounts of cellobiose and cellotriose
?
carboxymethylcellulose + H2O
additional information
-
-
-
endoglucanase 1b: cellotriose, 45%, cellotetraose, 31%, cellobiose, 17%, and cellopentaose, 8%. Endoglucanase 2: cellotriose, 50%, and cellotetraose, 30%
?
carboxymethylcellulose + H2O
additional information
-
-
catalytic domain of endoglucanase G
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
hydrolyzed by cellulase I and III, no hydrolysis with cellulase II
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
endoglucanase E1 exhibits higher activity
endoglucanase E2 produces glucose, cellobiose, cellotriose and higher oligosaccharides. The major end product produced by endoglucanase E1 is cellobiose and small amounts of glucose
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Bacillus sp. N-4
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Aspergillus niger IFO31125
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Talaromyces emersonii CBS 814.70
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
-
-
mainly glucose with small amounts of cellobiose and cellotriose
?
carboxymethylcellulose + H2O
additional information
-
Ruminococcus albus F40
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Aspergillus terreus GN1
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Fibrobacter succinogenes S85
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Fibrobacter succinogenes S85
-
catalytic domain of endoglucanase G
-
-
?
carboxymethylcellulose + H2O
additional information
-
Fibrobacter succinogenes S85
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Bacillus sp. No. 1139
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Myceliophthora heterothallica D-14
-
-
-
-
?
carboxymethylcellulose + H2O
additional information
-
Bacillus circulans F-2
-
-
-
-
?
cellohexaitol + H2O
?
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
additional information
-
-
-
-
?
cellohexaose + H2O
additional information
-
-
-
-
?
cellohexaose + H2O
additional information
-
-
-
-
-
?
cellohexaose + H2O
additional information
-
-
-
-
-
-
cellohexaose + H2O
additional information
-
-
-
-
-
?
cellohexaose + H2O
additional information
-
-
-
-
?
cellohexaose + H2O
additional information
-
Robillarda sp.
-
-
-
?
cellohexaose + H2O
additional information
-
Robillarda sp.
-
-
-
-
?
cellohexaose + H2O
additional information
-
-
-
cellotriose + cellobiose + cellotetraose
?
cellohexaose + H2O
additional information
-
-
-
cellotriose + cellobiose + cellotetraose
?
cellohexaose + H2O
additional information
-
-
-
cellobiose + cellotetraose
?
cellohexaose + H2O
additional information
-
Bacillus cellulyticus K-12
-
-
cellobiose + cellotetraose
?
cellohexaose + H2O
additional information
-
-
-
cellotriose + cellobiose + cellotriose
?
cellohexaose + H2O
additional information
-
-
-
cellotriose + glucose + cellobiose + cellotetraose
?
cellohexaose + H2O
additional information
-
-
-
cellotriose is formed and then slowly degraded
?
cellohexaose + H2O
additional information
-
-
cellulase A, B, and C
-
-
?
cellohexaose + H2O
additional information
-
-
-
-
-
?
cellopentaose
cellohexaose + celloheptaose
show the reaction diagram
Robillarda sp.
-
transfer reaction
-
?
cellopentaose + H2O
additional information
-
-
-
-
-
?
cellopentaose + H2O
additional information
-
-
-
-
-
?
cellopentaose + H2O
additional information
-
-
-
-
-
?
cellopentaose + H2O
additional information
-
Gluconacetobacter xylinus
-
-
-
-
?
cellopentaose + H2O
additional information
-
-
-
-
-
?
cellopentaose + H2O
additional information
-
-
-
-
-
?
cellopentaose + H2O
additional information
-
-
-
-
-
?
cellopentaose + H2O
additional information
-
Robillarda sp.
-
-
-
-
?
cellopentaose + H2O
additional information
-
Bacillus cellulyticus K-12
-
-
-
-
?
cellopentaose + H2O
additional information
-
-
-
cellobiose + cellotriose
?
cellopentaose + H2O
additional information
-
-
-
cellobiose + cellotriose
?
cellopentaose + H2O
additional information
-
-
-
cellobiose + cellotriose
?
cellopentaose + H2O
additional information
-
-
-
cellotetraose + glucose
?
cellopentaose + H2O
additional information
-
-
-
cellobiose + cellotriose + glucose
?
cellopentaose + H2O
additional information
-
-
enzyme form EGB and EGC
-
-
?
cellopentaose + H2O
additional information
-
-
cellulase A, B, and C
-
-
?
cellopentaose + H2O
additional information
-
-
beta-configuration of the anomeric carbon atoms is retained
-
-
?
cellopentaose + H2O
additional information
-
-
hydrolyzed by endo-beta-1,4-glucanase component YEG1 and YEG2
cellobiose + cellotriose
?
cellopentaose + H2O
additional information
-
-
-
-
-
?
cellopentaose + H2O
additional information
-
Ruminococcus albus F40
-
-
-
-
?
cellopentaose + H2O
additional information
-
Bacillus circulans F-2
-
-
cellobiose + cellotriose
?
microcrystalline cellulose + H2O
?
show the reaction diagram
-
endoglucanase 35, endoglucanase 47
-
-
?
additional information
?
-
Penicillium funiculosum
-
-
-
-
-
additional information
?
-
-
no hydrolysis of laminarin
-
-
?
additional information
?
-
-
no hydrolysis of laminarin
-
-
?
additional information
?
-
-
hydrolysis of cotton
-
-
-
additional information
?
-
-
hydrolysis of cotton
-
-
-
additional information
?
-
-
hydrolysis of cotton
-
-
-
additional information
?
-
-
hydrolysis of cotton
-
-
-
additional information
?
-
-
the low-molecular weight enzyme form is able to form free fibres from filter paper
-
-
-
additional information
?
-
-
no activity with p-nitrophenyl-beta-D-glucoside
-
-
?
additional information
?
-
-
no activity with p-nitrophenyl-beta-D-glucoside
-
-
?
additional information
?
-
-
trans-glucosidase activity also observed
-
-
?
additional information
?
-
-
hydrolysis of phosphoric-acid swollen filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
-
hydrolysis of filter paper
-
-
-
additional information
?
-
Bacillus cellulyticus K-12
-
hydrolysis of filter paper
-
-
-
additional information
?
-
Penicillium funiculosum
-
beta-1,3-linkages, alpha-1,6-linkages and beta-1,6-linkages are also susceptible to hydrolysis
-
-
?
additional information
?
-
-
no activity with cellobiose
-
-
?
additional information
?
-
-
no activity with cellobiose
-
-
?
additional information
?
-
-
no activity with cellobiose
-
-
-
additional information
?
-
-
no activity with cellobiose
-
-
?
additional information
?
-
-
no activity with cellobiose
-
-
?
additional information
?
-
-
no activity with cellobiose
-
-
?
additional information
?
-
-
when the oligosaccharides are modified by reduction with sodium borotritide, the second linkage from the reducing end becomes significantly susceptible to the enzyme and is preferentially cleaved
-
-
?
additional information
?
-
-
Arg78 participates in catalysis
-
-
-
additional information
?
-
-
modular enzyme that contains a family 30 carbohydrate-binding modules, CBM, and a family 9 catalytic module at its N-terminal moiety. The CBM is extremely important not only because it mediates the binding of the enzyme to the substrate but also because it participates in the catalytic function of the enzyme or contributes to maintain the correct tertiary structure of the family 9 catalytic module for expressing enzyme activity
-
-
-
additional information
?
-
-
no activity with avicell, xylan, galactan, arabinan, mannan or laminarin
-
-
-
additional information
?
-
-
no activity with crystalline cellulose or cellobiose
-
-
-
additional information
?
-
-
no activity with laminarin, avicel, pullulan and pachyman
-
-
-
additional information
?
-
Q46002
no activity with p-nitrophenyl cellobiose
-
-
-
additional information
?
-
Q9STD9
no activity with tamarind xyloglucan, barley (1-3)(1-4)-beta-D-glucan, wheat arabinoxylan or birchwood xylan
-
-
-
additional information
?
-
-
no cleavage of cellotetraose, cellotriose and p-nitrophenyl-cellobiose
-
-
-
additional information
?
-
-
no hydrolysis of cellobioside, cellotrioside, cellotetraoside, laminarin, curdlan, xylan, p-nitrophenyl beta-D-cellotrioside and p-nitrophenyl beta-D-cellotetraoside
-
-
-
additional information
?
-
-
no hydrolysis of cellulose, cotton, oat splet xylan and birchwood xylan
-
-
-
additional information
?
-
Q9X273
transglycosylation activity with smaller soluble cellooligosaccharides
-
-
-
additional information
?
-
-
effect of carbon source on enzyme activity
-
-
-
additional information
?
-
-
possible role of the enzyme in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening. mRNA abundance is down-regulated in response to fungal infection. It is rapidly reduced 1 day after pathagen infection and completely suppressed 4 days after infection
-
-
-
additional information
?
-
-
endo-beta-1,4-glucanase EG27 shows no activity with p-nitrophenyl-beta-D-cellobiose, salicin and starch, endo-beta-1,4-glucanase EG45 shows no activity with p-nitrophenyl-beta-D-cellobiose, salicin and starch
-
-
-
additional information
?
-
-
no degradation of oat spelt xylan and larch wood xylan
-
-
-
additional information
?
-
Q3LHN3
the enzyme consists of an N-terminal signal peptide, two glycosyl hydrolase family 5 catalytic modules, two novel carbohydrate-binding modules, two linker sequences, and a C-terminal sequence with an unknown function. Removal of the carbohydrate-binding modules from rCel5A reduces the catalytic activities with various polysaccharides remarkably
-
-
-
additional information
?
-
-
Cel9A does not cleave xylan
-
-
-
additional information
?
-
Penicillium sp., Trichoderma sp.
-
cellulase preparations that perform best on hardwood also show superior performance on the softwood substrates
-
-
-
additional information
?
-
-
distribution of cellulase between the residual substrate and supernatant during the course of enzymatic hydrolysis of steam-exploded wheat straw
-
-
-
additional information
?
-
-
enzymatic deinking experiments, the ink removal rate in samples treated with the catalytic module is only slightly higher (about 8%), than that of untreated controls, whereas that of the EG1-treated samples is 100% higher. Bio-stoning of denim with EG1-CM results in increases of 48% and 40% in weight loss and indigo dye removal, respectively compared with untreated controls. These increases are considerably lower than the corresponding values of 219% and 133% obtained when samples are treated with EG1
-
-
-
additional information
?
-
-
no activity against crystalline forms of cellulose such as filter-paper or avicel and the beta-1,3-linked glucan laminarin. No activity with xylan
-
-
-
additional information
?
-
Q3ZMA8
no activity on Avicel or xylan
-
-
-
additional information
?
-
-
no activity with p-nitrophenyl-beta-D-glucopyranoside
-
-
-
additional information
?
-
-
no hydrolysis of cellobiose
-
-
-
additional information
?
-
Q25C15
the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose
-
-
-
additional information
?
-
-
the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all
-
-
-
additional information
?
-
-
the hydrolase exhibited chitosanase activity and cellulase activity
-
-
-
additional information
?
-
Q9PF60, -
Xf818 carries out transglycosylation. The enzyme is unable to hydrolyse alpha-cellulose and laminarin (a beta-1,3 linked glucan)
-
-
-
additional information
?
-
-
among the processings of medicinal and aromatic plants, distillation waste of Cymbopogon winterianus, and bioprocessings of Artemisia annua (an industrial pharmaceutically important plant and source of artemisnin, an antimalarial compound) are selected on the basis of their capability to support higher levels of production of total cellulases, whereas garden waste, primarily consisting of Cynodon dactylon, is considered as the control, representing other lignocellulosic waste. Marc of Artemisia annua, a waste produced in huge amounts after the processing of the Artemisia annua herb, is found to be the suitable substrate for this fungus for maximizing the production of all three constituents of cellulase
-
-
-
additional information
?
-
-
beta-1,4-endoglucanases show activities of the cleavage of the cellulose backbone due to an endocleavage. Oligosaccharides of different lengths as well as cellobiose and glucose are formed
-
-
-
additional information
?
-
-
enzyme is essential for growth and development of potato cyst nematodes
-
-
-
additional information
?
-
A4UU22
carboxymethyl cellulose sodium salt is a better substrate than insoluble cellulose substrate such as Avicel (0 U/mg), SIGMACELL cellulose (0 U/mg), and CM cellulose
-
-
-
additional information
?
-
Q8J0K6, Q8J0K7, Q8J0K8
Cel7B has greater activity than the endoglucanases Cel45A and Cel7A against crystalline cellulose, whereas in the case of amorphous substrate the order is reversed
-
-
-
additional information
?
-
-
cellobiose and xylan are no substrates
-
-
-
additional information
?
-
Q83XK5
enzyme does not release reducing sugars from Avicel microcrystalline cellulose, cellobiose, polygalacturonate, locust bean gum, xylan, Rhizobium leguminosarum bv. trifolii acidic heteropolysaccharide EPS types I, II, or III, or Sinorhizobium meliloti succinoglycan exopolysaccharide. Enzyme has a high substrate specificity for noncrystalline cellulose
-
-
-
additional information
?
-
-
free cellulase compared with cellulase immobilized onto Si wafers and amino-terminated surfaces, amount of glucose produced by free cellulase is about 20% higher than that obtained from immobilized cellulase
-
-
-
additional information
?
-
-, Q9BLD2
hydrolyzing internal beta-1,4-glycosidic bonds and resulting in a smear of polymers with different lengths. The hydrolytic products of tCfEG are one unit sugar less than those produced by nCfEG
-
-
-
additional information
?
-
-
investigation of substrate specifity, starch as a substrate shows a relative activity of 0% and xlycan shows a relative activity of 0.9%
-
-
-
additional information
?
-
-
wild-type and mutant can not hydrolyze Avicel, laminarin and chitosan
-
-
-
additional information
?
-
-
glucanase hydrolyses beta-1,4-glycosidic bonds
-
-
-
additional information
?
-
-
glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds
-
-
-
additional information
?
-
D2DRB6
Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan
-
-
-
additional information
?
-
-
the fungal enzyme binds specifically to cyst wall cellulose of Acanthamoeba ssp., which causes keratitis in human cornea, overview
-
-
-
additional information
?
-
-
carboxymethyl cellulose and beta-glucan are the best substrates with relative activities of 100% and 83%, respectively, birch wood xylan and oat spelt xylan have moderate activities of 68% and 60%, respectively, which otherwise supports low level of cellulase activity, Avicel has the lowest activity of 12%, substrate specificity, overview
-
-
-
additional information
?
-
-
CcCel6C exhibits high cellobiohydrolase activity
-
-
-
additional information
?
-
-
inverting glycoside hydrolases catalyze bond cleavage using a single-displacement mechanism involving the participation of two acidic amino acid residues positioned opposite each other across the active site cleft or tunnel
-
-
-
additional information
?
-
D2DRB6
poor activity with laminarin and oat spelt xylan, no activity with Avicel
-
-
-
additional information
?
-
-
substrates are acid swollen cellulose, lichenan, beta-glucan, carboxymethyl cellulose, galactomannans, oat spelt xylan, avicel, and steam exploded bagasse, no activity glucomannan and laminarin
-
-
-
additional information
?
-
-
Cel9A presents both exo- and endo-cellulase activities, reaction mode, existence of an active conformer prior to ligand binding, overview
-
-
-
additional information
?
-
F6M085
EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside
-
-
-
additional information
?
-
-
the enzyme shows multisubstrate specificity, showing significantly higher activity with lichenan and beta-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse
-
-
-
additional information
?
-
Bacillus sp., Geobacillus sp.
-
the organism possesses carboxymethyl cellulase and cellulase activities, overview
-
-
-
additional information
?
-
G3BJX5
Rucel5B is active towards carboxymethyl cellulose, barley glucan, lichenan, pNPC, PASC, avicel and Whatman NO.1 filter paper, but not active towards laminarin, beta-1,6-glucan and birchwood xylan
-
-
-
additional information
?
-
Q60032
substrate-binding mode of cellulase 12A, overview. A network of interactions exists between the enzyme and its substrate. The sugar residues bound to the enzyme appear to be more ordered in the 22 and 21 subsites than in the 11, 12 and 23 subsites. In the E134C crystals the bound 21 sugar at the cleavage site consistently show the alpha-anomeric configuration, implicating an intermediate-like structure
-
-
-
additional information
?
-
O58925
with substrate cellulose, the substrate position is fixed by the alignment of one cellobiose unit between the two aromatic amino acid residues at subsites +1 and +2. During the enzyme reaction, the glucose structure of cellulose substrates is distorted at subsite -1, and the beta-1,4-glucoside bond between glucose moieties is twisted between subsites -1 and +1. Subsite -2 specifically recognizes the glucose residue, but recognition by subsites +1 and +2 is loose during the enzyme reaction. This type of recognition is important for creation of the distorted boat form of the substrate at subsite -1
-
-
-
additional information
?
-
-
EglA hydrolyzes shorter cellodextrins (DP <5) as well as the amorphous portions of polysaccharides which contain only beta-1,4 bonds such as carboxymethyl cellulose, microcrystalline cellulose, Whatman paper, and cotton linter. Kinetics studies with cellooliogsaccharides and p-nitrophenyl-cellooligosaccharides indicated that the enzyme had three glucose binding subsites (-I, -II, and -III) for the nonreducing end and two glucose binding subsites (+I and +II) for the reducing end from the scissile glycosidic linkage. No activity is detected on solely beta-1,3-linked oligosaccharides or polysaccharides
-
-
-
additional information
?
-
Q97YG7, -
no hydrolysis of p-nitrophenyl-beta-D-cellobioside and p-nitrophenyl-beta-D-cellotrioside, no exocellulase activity
-
-
-
additional information
?
-
Q97X08, -
the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values
-
-
-
additional information
?
-
-
the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (8090% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes
-
-
-
additional information
?
-
Clostridium thermocellum CelJ
-
modular enzyme that contains a family 30 carbohydrate-binding modules, CBM, and a family 9 catalytic module at its N-terminal moiety. The CBM is extremely important not only because it mediates the binding of the enzyme to the substrate but also because it participates in the catalytic function of the enzyme or contributes to maintain the correct tertiary structure of the family 9 catalytic module for expressing enzyme activity
-
-
-
additional information
?
-
Bacillus agaradhaerens JAM-KU023
-
the enzyme does not degrade curdran, xylan, laminarin and pectin (0.5% each) at all
-
-
-
additional information
?
-
Mucor circinelloides NRRL 26519
-
no degradation of oat spelt xylan and larch wood xylan
-
-
-
additional information
?
-
Bacillus sp. CTP-09
-
investigation of substrate specifity, starch as a substrate shows a relative activity of 0% and xlycan shows a relative activity of 0.9%
-
-
-
additional information
?
-
Cellulomonas flavigena 531
Q3ZMA8
no activity on Avicel or xylan
-
-
-
additional information
?
-
Desulfurococcaceae EBI-244
F6M085
EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside
-
-
-
additional information
?
-
Rhizobium leguminosarum bv. trifolii ANU843
Q83XK5
enzyme does not release reducing sugars from Avicel microcrystalline cellulose, cellobiose, polygalacturonate, locust bean gum, xylan, Rhizobium leguminosarum bv. trifolii acidic heteropolysaccharide EPS types I, II, or III, or Sinorhizobium meliloti succinoglycan exopolysaccharide. Enzyme has a high substrate specificity for noncrystalline cellulose
-
-
-
additional information
?
-
Penicillium brasilianum IBT 20888
-
effect of carbon source on enzyme activity
-
-
-
additional information
?
-
Cherax destructor Clark
-
glucanase hydrolyses beta-1,4-glycosidic bonds
-
-
-
additional information
?
-
Bacillus subtilis AS3
-
the enzyme shows multisubstrate specificity, showing significantly higher activity with lichenan and beta-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse
-
-
-
additional information
?
-
Talaromyces emersonii CBS 814.70
-
hydrolysis of phosphoric-acid swollen filter paper, hydrolysis of filter paper
-
-
-
additional information
?
-
Aspergillus terreus GN1
-
hydrolysis of filter paper
-
-
-
additional information
?
-
Bacillus sp. DUSELR13
-
the organism possesses carboxymethyl cellulase and cellulase activities, overview
-
-
-
additional information
?
-
Fibrobacter succinogenes S85
-
no hydrolysis of laminarin
-
-
?
additional information
?
-
Q25C15
the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose
-
-
-
additional information
?
-
Bacillus sp. No. 1139
-
trans-glucosidase activity also observed
-
-
?
additional information
?
-
Bacillus subtilis JA18
-
wild-type and mutant can not hydrolyze Avicel, laminarin and chitosan
-
-
-
additional information
?
-
Gecarcoidea natalis Pocock 1888
-
glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds
-
-
-
additional information
?
-
Q97X08, -
the enzyme does not hydrolyse 4-nitrophenyl beta-D-cellobioside and 4-nitrophenyl beta-D-cellotrioside. No activity could be detected with crystalline cellulose at both acidic and neutral pH values
-
-
-
additional information
?
-
Q97YG7
the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (8090% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes
-
-
-
additional information
?
-
Bacillus circulans KSM-N257
-
no hydrolysis of cellobioside, cellotrioside, cellotetraoside, laminarin, curdlan, xylan, p-nitrophenyl beta-D-cellotrioside and p-nitrophenyl beta-D-cellotetraoside
-
-
-
additional information
?
-
Geobacillus sp. WSUCFI
-
the organism possesses carboxymethyl cellulase and cellulase activities, overview
-
-
-
additional information
?
-
Myceliophthora heterothallica D-14
-
hydrolysis of cotton, hydrolysis of filter paper
-
-
-
additional information
?
-
Bacillus amyloliquefaciens DL-3
-
no activity with p-nitrophenyl-beta-D-glucopyranoside
-
-
-
additional information
?
-
Solanum lycopersicum Mill.
-
possible role of the enzyme in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening. mRNA abundance is down-regulated in response to fungal infection. It is rapidly reduced 1 day after pathagen infection and completely suppressed 4 days after infection
-
-
-
additional information
?
-
Penicillium decumbens JUA10
-
distribution of cellulase between the residual substrate and supernatant during the course of enzymatic hydrolysis of steam-exploded wheat straw
-
-
-
additional information
?
-
Bacillus circulans F-2
-
hydrolysis of filter paper
-
-
-
xylan + H2O
?
show the reaction diagram
Bacillus amyloliquefaciens DL-3
-
22.5% of the activity with carboxymethylcellulose
-
-
?
xylan + H2O
additional information
-
-
-
-
-
?
xylan + H2O
additional information
-
-
-
-
-
?
xylan + H2O
additional information
-
Penicillium funiculosum
-
-
-
-
?
xylan + H2O
additional information
-
Lenzites trabea
-
-
-
-
?
xylan + H2O
additional information
-
-
-
main products: cellobiose + cellotetraose
?
xylan + H2O
additional information
-
-
no activity
-
-
-
xylan + H2O
additional information
-
-
endoglucanase B
-
-
?
xylan + H2O
additional information
-
-
hydrolysis with endoglucanase B, no action with endoglucanase A and C
-
-
?
xylan + H2O
additional information
-
-
6% of the activity with barley beta-glucan with the insoluble xylan from oats spelt, 27% of the activity with barley beta-glucan with the soluble xylan from oats spelt, catalytic domain of endoglucanase G
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Geobacillus sp.
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Acremonium cellulolyticus, Acremonium cellulolyticus CF-2612
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Bacillus sp. DUSELR13, Geobacillus sp. WSUCFI
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Brevibacillus sp.
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Geobacillus sp.
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
C5I945, C5I946
-
-
-
?
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
-
-
-
?
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
inducible enzyme
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
induced by carboxymethylcellulose, non-constitutive synthesis
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
Sporotrichum pulverulentum
-
enzyme is involved in degradation of cellulose
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
enzyme plays an important role in the carbon cycle
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
in bean the 9.5 cellulase appears to function in the shedding of various organs such as fruits, flowers, and leaves. The acid cellulases function in loosening the cellulose fibrils of the cell wall to allow expansion and growth
-
-
-
cellulose + H2O
cellooligosaccharide
show the reaction diagram
-
the enzyme plays an important role in maintaining the carbon balance in nature
-
-
-
cellulose + H2O
cellobiose
show the reaction diagram
O76130, -
-
-
-
?
cellulose + H2O
cellobiose + ?
show the reaction diagram
Q97X08, -
the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Acremonium cellulolyticus CF-2612
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Marinobacter sp. MSI032
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Bacillus sp. DUSELR13
-
-
-
-
?
cellulose + H2O
cellobiose + ?
show the reaction diagram
Q97X08, -
the secreted protein enables Sulfolobus solfataricus to use cellulose as an external carbon source
-
-
?
cellulose + H2O
?
show the reaction diagram
Geobacillus sp. WSUCFI
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
Bacillus subtilis LN
C5I945, C5I946
-
-
-
?
cellulose + H2O
cellooligosaccharide
show the reaction diagram
Myceliophthora heterothallica D-14
-
enzyme plays an important role in the carbon cycle
-
-
-
noncrystalline cellulose + H2O
?
show the reaction diagram
-
as natural substrate for CEL7
-
-
?
xyloglucan + H2O
?
show the reaction diagram
-
as natural substrate in CEL7
-
-
?
crystalline cellulose + H2O
?
show the reaction diagram
-
as natural substrate for CEL9C1
-
-
?
additional information
?
-
-
effect of carbon source on enzyme activity
-
-
-
additional information
?
-
-
possible role of the enzyme in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening. mRNA abundance is down-regulated in response to fungal infection. It is rapidly reduced 1 day after pathagen infection and completely suppressed 4 days after infection
-
-
-
additional information
?
-
-
among the processings of medicinal and aromatic plants, distillation waste of Cymbopogon winterianus, and bioprocessings of Artemisia annua (an industrial pharmaceutically important plant and source of artemisnin, an antimalarial compound) are selected on the basis of their capability to support higher levels of production of total cellulases, whereas garden waste, primarily consisting of Cynodon dactylon, is considered as the control, representing other lignocellulosic waste. Marc of Artemisia annua, a waste produced in huge amounts after the processing of the Artemisia annua herb, is found to be the suitable substrate for this fungus for maximizing the production of all three constituents of cellulase
-
-
-
additional information
?
-
-
beta-1,4-endoglucanases show activities of the cleavage of the cellulose backbone due to an endocleavage. Oligosaccharides of different lengths as well as cellobiose and glucose are formed
-
-
-
additional information
?
-
-
enzyme is essential for growth and development of potato cyst nematodes
-
-
-
additional information
?
-
D2DRB6
Bgl7A can effectively hydrolyze beta-1,4 bonds and some beta-1,3 linkages in beta-glucan. Belonging to the group of non-specific endoglucanase, Bgl7A can hydrolyze not only beta-glucan and cellulose but also laminarin and oat spelt xylan
-
-
-
additional information
?
-
-
the fungal enzyme binds specifically to cyst wall cellulose of Acanthamoeba ssp., which causes keratitis in human cornea, overview
-
-
-
additional information
?
-
-
Cel9A presents both exo- and endo-cellulase activities, reaction mode, existence of an active conformer prior to ligand binding, overview
-
-
-
additional information
?
-
F6M085
EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside
-
-
-
additional information
?
-
-
the enzyme shows multisubstrate specificity, showing significantly higher activity with lichenan and beta-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse
-
-
-
additional information
?
-
Bacillus sp., Geobacillus sp.
-
the organism possesses carboxymethyl cellulase and cellulase activities, overview
-
-
-
additional information
?
-
Desulfurococcaceae EBI-244
F6M085
EBI-244 is active on a range of high molecular weight carbohydrate substrates containing beta-1,4-linked glucose, including carboxymethyl cellulose, Avicel, and filter paper, it is active toward 4-nitrophenyl-cellobioside but inactive toward 4-nitrophenyl-glucoside
-
-
-
additional information
?
-
Penicillium brasilianum IBT 20888
-
effect of carbon source on enzyme activity
-
-
-
additional information
?
-
Bacillus subtilis AS3
-
the enzyme shows multisubstrate specificity, showing significantly higher activity with lichenan and beta-glucan and lower activity with laminarin, hydroxyethylcellulose, and steam exploded bagasse
-
-
-
additional information
?
-
Bacillus sp. DUSELR13, Geobacillus sp. WSUCFI
-
the organism possesses carboxymethyl cellulase and cellulase activities, overview
-
-
-
additional information
?
-
Solanum lycopersicum Mill.
-
possible role of the enzyme in the softening of pericarp tissue and in the liquefaction of locules that takes place during ripening. Cel1 EGase responds to pathogen infection and supports a relationship between EGases, plant defense responses and fruit ripening. mRNA abundance is down-regulated in response to fungal infection. It is rapidly reduced 1 day after pathagen infection and completely suppressed 4 days after infection
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ag+
-
stimulates
Ba2+
Q25C15
1 mM, 1.3fold activation
Br-
-
activates
Ca2+
-
CaCl2 or MgCl2 required
Ca2+
-
stimulates activity of endoglucanase E1
Ca2+
-
stimulates
Ca2+
-
strong stimulation of cellulose-solubilizing activity, no effect on carboxymethylcellulase activity
Ca2+
Bacillus cellulyticus K-12
-
stimulates
Ca2+
-
stimulates
Ca2+
-
enhances activity
Ca2+
Q9STD9
activity of mutant enzyme DELTA1-90 is enhances in a concentration-dependent manner up to about 40 mM
Ca2+
-
2 mM, 1.5fold activation
Ca2+
Q25C15
1 mM, 1.3fold activation
Ca2+
-
activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold
Ca2+
-
activates
Ca2+
-
the thermostability of the enzyme is maintained in part by the binding of Ca2+ in a DxDxDG Ca2+-binding motif, atypical of most archaeal proteins
CaCl2
-
stimulation to 122% of control
CaCl2
-
5 mM, activation to 109% of control
Cl-
-
60 mM, activates
Co2+
-
increases activity by 139.5%
Co2+
-
2 mM, slight activation, cellulase I
Co2+
-
2 mM, 40% stimulation
Co2+
-
1 mM, increase activity by 38%
Co2+
-
enhances activity
Co2+
G3BJX5
activates
CoCl2
-
0.5 mM, activity is enhanced by 44%
CoCl2
-
stimulation to 137% of control
CrCl3
-
stimulation to 119% of control
Cu2+
-
activates
Cu2+
-
activates
Fe2+
-
enhances activity
Fe3+
-
5 mM, 15% activation
Fe3+
-
activates
Hg2+
-
activates
K+
-
enhances activity
KCl
-
slight activation
KCl
-
25 mM, activation
KCl
-
5 mM, 15% stimulation
KCl
-
0.8 M, 4.23fold activation
LiCl
-
0.05 M, 1.9fold activation
Mg2+
-
MgCl2 or CaCl2 required
Mg2+
-
stimulates activity of endoglucanase E1
Mg2+
-
stimulates
Mg2+
-
strong stimulation of cellulose-solubilizing activity, no effect on carboxymethylcellulase activity
Mg2+
Bacillus cellulyticus K-12
-
stimulates hydrolysis of avicel and carboxymethylcellulose
Mg2+
-
stimulates
Mg2+
-
stimulates
Mg2+
-
10-30 mM, stimulates
Mg2+
-
2 mM, 1.48fold activation
Mg2+
Q25C15
1 mM, 1.3fold activation
Mg2+
-
slightly activating
Mg2+
-
activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold
Mg2+
-
activates
Mg2+
G3BJX5
activates
MgCl2
-
5 mM, activity is enhanced by 27%
Mn2+
-
strong stimulation of cellulose-solubilizing activity, no effect on carboxymethylcellulase activity
Mn2+
-
activates
Mn2+
-
enhances activity
Mn2+
-
1 mM, 1.3fold activation
Mn2+
-
the removal of metals results into complete loss of enzymatic activity and is completely recovered in the presence of 1 mM Mn2+. 12fold activation at 1.5 mM Mn2+
Na+
-
enhances activity
Na+
-
10-30 mM, stimulates
Na+
-
activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold
Na+
-
activate sat 0-20% v/w, optimally at 5% v/w
Na2SO4
-
0.5 M, 4.43fold activation
NaBr
-
0.5 M, 3.84fold activation
NaCl
-
25 mM, activation
NaCl
-
5 mM, 15% stimulation
NaCl
-
the enzyme activity increass around 4fold by the addition of 0.2-2.0 M NaCl in 0.1 M glycine-NaOH buffer (pH 9.0)
NaCl
-
100% activation at 2.5 M
NaNO3
-
0.5 M, 4fold activation
Ni2+
-
activates the truncated enzyme mutant CtLic26A-Cel5 about 2fold
Ni2+
G3BJX5
activates
Sr2+
-
2 mM, 1.45fold activation
Zn2+
Q25C15
1 mM, 1.3fold activation
Zn2+
-
highly activating
Zn2+
-
activates
Zn2+
-
zinc ions tightly bound between the two catalytic glutamate residues, which present an obstacle for the entrance of ligand in the active site
ZnCl2
-
slight activation
ZnCl2
-
2 mM, slight activation, cellulase I
Mn2+
-
activates
additional information
-
no effect by F-, I- and NO3-
additional information
-
no inhibition up to 7 mM of Ca2+, Zn2+ and K+ is observed
additional information
-
effect of metal ions tested: Ca2+, Mg2+, Hg2+, Fe2+, Cu2+, Zn2+, SDS, dithiothreitol and metal chelator such as EDTA
additional information
-
enzyme activity is not affected by Hg2+
additional information
D2DRB6
the enzymatic activity is enhanced or not affected by the presence of Na+, K+, Li+, Ca2+, or 2-mercaptoethanol
additional information
G3BJX5
no effect by Zn2+ on Rucel5B activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-butyl-3-methylimidazolium methylsulfate
-
the enzyme precipitates immediately on addition to the reaction mixture
-
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
Q25C15
100 mM, 98% inhibition
2,4-Dinitrophenol
-
-
4-hydroxybenzoic acid
-
-
acetonitrile
-
10% v/v, 89% loss of activity
Ag+
-
AgNO3, endoglucanase E1 and E2
Ag+
-
1 mM, 75% inhibition
Ag+
-
1 mM, partial inhibition
Ag+
-
0.1 mM, strong inhibition
alpha-chymotrypsin
-
denaturant
-
Ba2+
Robillarda sp.
-
-
Ba2+
-
1 mM, 79%inhibition
Ca2+
-
slight inhibition of endoglucanase E2 activity
Ca2+
-
1 mM, slight inhibition
Ca2+
-
1 mM, 74%inhibition
Carboxymethylcellulose
-
binding of the cellulose-binding domain to avicel is inhibited by carboxymethylcellulose, but not by barley beta-glucan and glucose at concentration of 0.1% and 0.5%
-
Cd2+
-
1 mM CdCl2, complete inhibition
cellobiose
-
51000 Da subunit from the multicomponent cellulase complexes
cellobiose
-
80 mM, 14% loss of activity, endoglucanase Z
cellobiose
-
inhibition of endoglucanase E3a, E3b and E4
cellobiose
Bacillus cellulyticus K-12
-
inhibits hydrolysis of carboxymethylcellulose and avicel
cellobiose
-
5 mM, complete inhibition of carboxymethyl cellulose hydrolysis
cellobiose
-
inhibits hydrolysis of carboxymethylcellulose and avicel
cellobiose
-
endoglucanase Cel 5A; endoglucanase Cel 7B
cellobiose
Q8J0K6, Q8J0K7, Q8J0K8
very low activity against 4-methyl-umberriferyl-beta-D-lactoside is almost totally inhibited by 0.005 M cellobiose
cellobiose
-
the non-complete saccharification of cellulose by thr enzyme seems to be due to product-feedback inhibition by cellobiose
Co2+
-
1 mM CoCl2, 44% inhibition
CoCl2
-
5 mM, 35% loss of activity
Cu2+
-
-
Cu2+
-
1 mM CuCl2, 41% inhibition
Cu2+
-
inhibition of cellulose hydrolysis, no carboxymethylcellulose hydrolysis
Cu2+
-
1 mM, partial inhibition
Cu2+
Bacillus cellulyticus K-12
-
CuCl2
Cu2+
-
1 mM CuCl2, 55% inhibition
Cu2+
-
5 mM, about 30% inhibition
Cu2+
-
1 mM, 12% loss of activity
CuCl2
-
82% inhibition
D-gluconic acid lactone
-
strong inhibition of membrane-bound cellulase
D-glucono-1,5-lactone
-
-
Dextran
-
partial inhibition of membrane-bound cellulase
diethyldicarbonate
-
-
Dimethylsulfoxide
-
30% v/v, 50% inhibition
dithiothreitol
-
78% inhibition
EDTA
-
1 mM, 55% inhibition
EDTA
-
5 mM, 40% inhibition
EDTA
Q9STD9
5 mM, complete inhibition of mutant enzyme DELTA1-90
EDTA
-
53% inhibition
EDTA
-
5 mM, 75% loss of activity
EDTA
-
50 mM, 14% inhibition
EDTA
-
78% inhibition
ethanol
-
30% v/v, 92% loss of activity
Fe2+
-
1 mM, 55% inhibition
-
Fe2+
-
1 mM FeCl2, 13% inhibition
-
Fe2+
-
2 mM, 67% inhibition
-
Fe2+
-
25% inhibition
-
Fe3+
Robillarda sp.
-
-
-
Fe3+
-
1.0 mM, significant inhibition
-
Fe3+
-
39% inhibition by 0.1 mM, 52% inhibition by 1 mM
-
Fe3+
-
1 mM, 73% inhibition
-
Fe3+
D2DRB6
-
-
glucosamine
-
inhibition of CM-cellulase activity of enzyme E1, E3a, E3b and E4
glucose
-
moderate inhibition
glucose
-
162 mM, 23% loss of activity, endoglucanase Z
glucose
-
29 mM, 12% inhibition of endoglucanase E4, no inhibition of endoglucanase E1 and E3a
glucose
-
end-product inhibition, 70% loss of activity by 0.3% glucose
Glutaraldehyde
-
-
glutathione
-
slight
Hg2+
-
endoglucanase E1 and E2; HgCl2
Hg2+
Robillarda sp.
-
-
Hg2+
-
1 mM, 67% inhibition
Hg2+
-
1 mM HgCl2, complete inhibition
Hg2+
-
inhibition of cellulose hydrolysis and carboxymethylcellulose hydrolysis
Hg2+
-
1 mM, complete inhibition
Hg2+
-
1 mM, complete inactivation
Hg2+
-
2 mM, cellulase I
Hg2+
-
1 mM, strong inhibition
Hg2+
-
Hg(acetate)2, restored by Cys or Cl-
Hg2+
Bacillus cellulyticus K-12
-
HgCl2
Hg2+
-
1 mM HgCl2, complete inhibition
Hg2+
-
5 mM, 95% inhibition
Hg2+
-
1.0 mM, significant inhibition
Hg2+
-
21% inhibition at 1 mM
Hg2+
-
2 mM, 20% inhibition
Hg2+
Q25C15
1 mM, complete inhibition
Hg2+
-
78% inhibition
HgCl2
-
5 mM, 76% loss of activity
iodoacetamide
-
-
iodoacetamide
-
-
KCl
-
5 mM, 23% loss of activity
KMnO4
-
0.1 mM, complete inhibition of cellulase II-A
lactose
-
51000 Da subunit from the multicomponent cellulase complexes
laminaribiose
-
-
linear alkyl benzene sulfonate
B5BNY1, -
LAS
mercaptoethanol
-
-
mercaptoethanol
-
1 mM, slight inhibition
methanol
-
30% v/v, 62% loss of activity
methylcellulose
-
poor inhibitor of carboxymethylcellulase activity of enzyme form E3a, E3b and E4
-
Mg2+
-
slight inhibition of endoglucanase E2 activity
Mg2+
Robillarda sp.
-
weak inhibition
Mg2+
-
2 mM, slight inhibition
Mg2+
Q9STD9
above 10 mM
Mg2+
-
31% inhibition at 1 mM
Mn2+
-
1 mM MnCl2, 18% inhibition
Mn2+
-
5 mM, about 30% inhibition
Mn2+
-
54% inhibition at 0.1 mM, 71% inhibition at 1 mM
Mn2+
-
5 mM, 20% inhibition
Mn2+
-
above 5 mM
MnCl2
-
5 mM, 67% loss of activity
N-bromosuccinimide
Robillarda sp.
-
-
N-bromosuccinimide
-
0.001 mM, complete inactivation
N-bromosuccinimide
-
-
N-bromosuccinimide
-
-
N-bromosuccinimide
-
1 mM, 90% inhibition
N-bromosuccinimide
-
-
N-bromosuccinimide
-
5 mM, 56% loss of activity
N-bromosuccinimide
Q25C15
1 mM, 99% inhibition
NEM
-
70% inhibition of cellulase 4.5 at 10 mM, no effect on cellulase 4.8
NH4+
-
5 mM, 10% inhibition
Ni2+
-
1 mM NiCl2, 68% inhibition
NiCl2
-
5 mM, 40% loss of activity
o-phenanthroline
Q25C15
1 mM, 75% inhibition
p-chloromercuribenzoate
Q25C15
0.01 mM, 20% inhibition
Pb(CH3COO)2
-
34% inhibition at 1 mM
Pb2+
-
1 mM Pb(CH3COO)-, 36% inhibition
Pb2+
-
2 mM, slight inhibition
PbCl2
-
5 mM, 37% loss of activity
PCMB
-
0.1 mM, 90% loss of activity of cellulase 4.5, 50% loss of activity of cellulase 4.8
PCMB
Bacillus cellulyticus K-12
-
-
PCMB
-
0.4 M, 60% inhibition
phenylmercuric ion
-
-
-
PMSF
Q25C15
1 mM, 89% inhibition
SDS
-
0.25 mM, complete inactivation
SDS
Bacillus cellulyticus K-12
-
10 mM, 98% inhibition
SDS
-
1% v/v, almost complete loss of activity
SDS
-
78% inhibition
sodium dodecyl benzene sulfonate
-
-
sodium hypochlorite
-
-
sodium hypochlorite
B5BNY1, -
-
sodium picryl sulfate
-
2 mM, cellulase I
SrCl2
-
5 mM, 36% loss of activity
starch
-
inhibits hydrolysis of glucan
Teepol
-
detergent, 0.1%, 80% inhibition
-
trans-Cinnamic acid
-
-
Urea
-
denaturant
Zn2+
Robillarda sp.
-
-
Zn2+
-
1 mM ZnSO4, 88% inhibition
Zn2+
-
inhibition of cellulose hydrolysis, no inhibition of carboxymethylcellulose hydrolysis
Zn2+
Q9STD9
above 5 mM
Zn2+
-
1 mM, 78%inhibition
Zn2+
-
52% inhibition
ZnCl2
-
47% inhibition
Monoiodoacetic acid
Q25C15
1 mM, 10% inhibition
additional information
-
2,3-di-O-methylated regions in 2,3-di-O-methylated cellulose, having a trace amount of unsubstituted glucose units competitively inhibit the hydrolysis of glucose in other molecular chains
-
additional information
-
more than 50% of the original activity is retained after incubation with sodium alkyl sulfonate, sodium alkyl sulfate, polyoxyethylene alkyl sulfonate, alpha-olefin sulfonate
-
additional information
-
no inhibition by EDTA, EGTA, 1,10-phenanthroline, monoiodoacetate, NEM, DTNB, PCMB and diethylpyrocarbonate
-
additional information
-
no inhibition by N-bromosuccinimide and iodoacetamide
-
additional information
-
is minutely inhibited by anionic detergent and oxidizing agent comparable with inhibition by commercial enzyme
-
additional information
-
no inhibition by cellobiose
-
additional information
D2DRB6
BglA7 is highly resistant to both pepsin and trypsin
-
additional information
-
gallic acid, cinnamic acid, ferulic acid, 4-coumaric acid, sinapic acid, vanilin, syringaldehyde, and 4-hydroxybenzoic acid are poor inhibitors
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
slight activation
alpha-cyclodextrin
-
10 mM, activity is enhanced by 23%
arabitol
-
50 mM, stimulates
ascorbic acid
-
2 mM, slight activation, cellulase I
beta-glucosidase
-
addition of beta-glucosidase, to avoid the well-known inhibition of cellulase by cellobiose, markedly accelerates cellulose hydrolysis up to a ratio of activity units (beta-glucosidase/cellulase) of 20
-
Ca2+
-
40% enhanced activity
cellobiose
-
0.29 mM, stimulation
cellobiose
-
stimulates endoglucanase Cel 12A
CH3COONa
-
1.6 M, 4.45fold activation
cysteine
-
stimulates
Cysteine hydrochloride
-
slight activation
Cysteine hydrochloride
-
slight activation
Cysteine hydrochloride
-
strong stimulation
Dimethylsulfoxide
-
above 5-15% v/v
dithiothreitol
-
slight activation
dithiothreitol
-
5 mM, stimulates
dithiothreitol
-
slight activation
dithiothreitol
-
slight activation
EDTA
Q25C15
1 mM, 1.2fold activation
Erythritol
-
50 mM, stimulates
gamma-cyclodextrin
-
10 mM, activity is enhanced by 137%
glutathione
-
slight activation
glutathione
-
slightly enhanced
Gly
-
50 mM, stimulates
glycerol
-
50 mM, stimulates
histamine
-
20 mM, stimulates
hydroquinone
-
2 mM, slight activation, cellulase I
L-His
-
50 mM, stimulates
methylcellulose
-
0.1%, 20% stimulation of carboxymethylcellulase activity of enzyme E1
-
Mg2+
-
32% enhanced activity
NEM
Q25C15
1 mM, 1.4fold activation
NH4Cl
-
0.2 M, 3fold activation
NH4NO3
-
0.05 M, 2.8fold activation
mini-CbpA
-
i.e. repeated cohesin domains of a scaffolding protein, enhance activity
-
additional information
-
2,3-di-O-methylated regions in 2,3-di-O-methylated cellulose, having a trace amount of unsubstituted glucose units activates the scission of glucose in the same molecular chain
-
additional information
-
pretreatment of cellulose with ionic liquids such as 1-butyl-3-methylimidazolium chloride, 1-methylimidazolium chloride, and tris-(2-hydroxyethyl)-methylammonium methylsulfate results in more rapid conversion to glucose than hydrolysis with cellulose that is not pretreated
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.2038
-
2',4'-dinitrophenyl-beta-D-cellobiose
-
endoglucanase A
0.2085
-
2',4'-dinitrophenyl-beta-D-cellobiose
-
chimeric xylanase/endoglucanase
0.197
-
2,4-dinitrophenyl beta-D-cellobioside
P26221
50C, mutant enzyme E424G
0.29
-
2,4-dinitrophenyl beta-D-cellobioside
P26221
50C, mutant enzyme E424A
0.697
-
2,4-dinitrophenyl beta-D-cellobioside
P26221
50C, wild-type enzyme Cel9A-68
0.15
-
4-nitrophenyl cellobioside
-
pH 6.0, 95C
0.37
-
4-nitrophenyl cellobioside
-
pH 6.0, 70C
0.36
-
4-nitrophenyl cellopentaoside
-
pH 6.0, 95C
0.12
-
4-nitrophenyl cellotetraoside
-
pH 6.0, 95C
0.061
-
4-nitrophenyl cellotrioside
-
pH 6.0, 95C
1.08
-
Carboxymethyl cellulose
Geobacillus sp.
-
pH 5.0, 70C
-
0.33
-
Cellohexaose
-
cellulase II-A
0.381
-
Cellohexaose
-
-
0.45
-
Cellohexaose
-
-
0.72
-
Cellohexaose
-
-
0.15
-
Cellopentaose
-
cellulase II-A
0.28
-
Cellopentaose
-
cellulase II-B
0.46
-
Cellopentaose
-
cellulase II-A
0.56
-
Cellopentaose
-
-
0.561
-
Cellopentaose
-
-
6.7
-
Cellopentaose
-
-
7.24
-
Cellopentaose
-
-
0.04
-
Cellotetraose
-
cellulase II-A
0.42
-
Cellotetraose
-
cellulase II-B
0.67
-
Cellotetraose
-
-
8.85
-
Cellotetraose
-
-
15.4
-
Cellotetraose
-
-
1.63
-
cellotriose
-
cellulase II-B
0.0017
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 3, 80C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
-
0.00174
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH and temperature not specified in the publication, hybrid protein (CelA-SSO1949-CelA, consisting of 262 amino acids) based on beta-endoglucanase from Sulfolobus solfataricus P2 and beta-endoglucanase from Thermotoga maritima
-
0.0055
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 3, 80C, native enzyme
-
0.002
-
N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 1.8, 80C
-
0.35
-
p-nitrophenyl cellobiose
-
50C, mutant enzyme C372A/C412A
0.46
-
p-nitrophenyl cellobiose
-
50C, mutant enzyme C106A/C159A
0.76
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme DELTAQ1-G5
0.78
-
p-nitrophenyl cellobiose
-
50C, mutant enzyme C106A/C159A/C372A/C412A
0.88
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme I157A
0.92
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme T160A
0.95
-
p-nitrophenyl cellobiose
-
50C, wild-type enzyme
0.95
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, wild-type enzyme
1.12
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, wild-type enzyme
1.12
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme Y299F
1.13
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus
1.23
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme H161A
1.24
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme E201Q
1.37
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme G158A
1.44
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme lacking 5 residues at the C-terminus
1.55
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme E163A
1.58
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme P164A
1.9
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme C159A
2.4
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme D385N
2.76
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme H297N
4.57
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme I162A
5.85
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme R156A
6.69
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme H155A
1.5
-
p-nitrophenyl-beta-D-cellobioside
-
4C
4
-
p-nitrophenyl-beta-D-cellobioside
-
35C
0.24
-
p-nitrophenyl-beta-D-cellopentaoside
Q9X273
-
0.25
-
p-nitrophenyl-beta-D-cellotetraoside
Q9X273
-
3.11
-
Carboxymethyl cellulose
-
pH 5.0, 75C
-
additional information
-
Carboxymethylcellulose
-
Km of Egl330 and Egl499 are 1.73 x 10 mg/ml and 1.14 x 10 mg/ml, respectively. Vmax of Egl330 is 1.78fold higher than that of Egl499, increases from 284 U/mg to 786 U/mg. The catalytic efficiency (kcat/Km) of Egl330 is slightly higher than that of Egl499
-
2.94
-
cellotriose
-
-
additional information
-
cellulose
-
13 celluloses characterized for crystallinity and crystal width (by X-ray diffraction), wet porosity (by thermoporometry), and particle size (by light scattering)
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
Hypocrea rufa, Robillarda sp.
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
Lenzites trabea
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
immobilized enzyme
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
Bacillus cellulyticus K-12
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
Km-value for carboxyymethylcellulose is 7.1 g/L at pH 8.5 and at 40C
-
additional information
-
additional information
-
the KM-value for the carboxymethylcellulose Na salt is 4.6 mg/ml
-
additional information
-
additional information
-
the Km-values for barley beta-glucan and carboxymethylcellulose are 0.32 mg/ml and 0.5 mg/ml
-
additional information
-
additional information
-
KM-value for carboxymethylcellulose is 1.5 mg/ml; KM-value for carboxymethylcellulose is 1.7 mg/ml
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
KM-value for carboxymethyl cellulase is 11 g/l, KM-value for beta-glucan is 0.12 g/l, endoglucanase EG28; KM-value for carboxymethyl cellulase is 1.3 g/l, KM-value for beta-glucan is 1.14 g/l, endoglucanase EG44; KM-value for carboxymethyl cellulase is 5.4 g/l, KM-value for beta-glucan is 3.0 g/l, endoglucanase EG25
-
additional information
-
additional information
-
KM-value for carboxymethyl cellulase is 2.4 g/l, KM-value for beta-glucan is 1.8 g/l, endoglucanase EG51; KM-value for carboxymethyl cellulase is 4.6 g/l, KM-value for beta-glucan is 0.17 g/l, endoglucanase EG60; KM-value for carboxymethyl cellulase is 6.8 g/l, KM-value for beta-glucan is 0.46 g/l, endoglucanase EG47
-
additional information
-
additional information
-
KM-value for carboxymethyl cellulose: 3.5% w/v (enzyme from strain NIAB442), 2.5% w/v (enzyme from deoxyglucose- and streptomycin-resistant mutant 51 Smr)
-
additional information
-
additional information
-
Km for carboxymethyl cellulose is 20 mg/ml
-
additional information
-
additional information
-
KM-value for carboxymethyl cellulose is 45 mg/ml
-
additional information
-
additional information
-
Km-value for carboxymethyl cellulose is 16.07 mg/ml
-
additional information
-
additional information
-
Km-value is 0.1 mg/ml glucose for hydrolysis of carboxymethyl cellulose
-
additional information
-
additional information
-
KM-value for carboxymethylcellulase is 6.25 mg/ml at pH 5.0, 50C
-
additional information
-
additional information
-
KM value: 20 mg carboxymethylcellulose/ml. Vmax value: 854 U/mg protein, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose
-
additional information
-
additional information
-
for digestion of carboxymethyl cellulose KM-value: 5.4 mg/ml and Vmax-value: 3118.4 U/mg
-
additional information
-
additional information
Q83XK5
Km of 84.4 mg/ml for carboxymethyl cellulose as a substrate
-
additional information
-
additional information
-
Km-values: 4.8 mg/ml for carboxymethyl cellulose at 50 and 60C, 4.5 mg/ml for carboxymethyl cellulose at 70C, 4.2 mg/ml for carboxymethyl cellulose at 80C, and 18.4 mg/ml for amorphous cellulose
-
additional information
-
additional information
-
kinetics, native enzyme
-
additional information
-
additional information
G3BJX5
kinetics of Rucel5B with different substrates, overview
-
additional information
-
additional information
-
KM-values of N-terminal and C-terminal deletion mutants
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.358
-
2',4'-dinitrophenyl-beta-D-cellobiose
-
chimeric xylanase/endoglucanase
0.397
-
2',4'-dinitrophenyl-beta-D-cellobiose
-
endoglucanase A
0.02
-
2,4-dinitrophenyl beta-D-cellobioside
P26221
50C, wild-type enzyme Cel9A-68
0.07
-
2,4-dinitrophenyl beta-D-cellobioside
P26221
50C, mutant enzyme E424A
0.7
-
2,4-dinitrophenyl beta-D-cellobioside
P26221
50C, mutant enzyme E424G
0.79
-
4-nitrophenyl cellobioside
-
pH 6.0, 95C
9.55
-
4-nitrophenyl cellobioside
-
pH 6.0, 70C
1.4
-
4-nitrophenyl cellopentaoside
-
pH 6.0, 95C
3.6
-
4-nitrophenyl cellotetraoside
-
pH 6.0, 95C
8.3
-
4-nitrophenyl cellotrioside
-
pH 6.0, 95C
3.8
-
beta-Glucan
-
50C, pH 5.5, endoglucanase EG25
16
-
beta-Glucan
-
50C, pH 5.5, endoglucanase EG47
18
-
beta-Glucan
-
50C, pH 5.5, endoglucanase EG60
64
-
beta-Glucan
-
50C, pH 5.5, endoglucanase EG28
76
-
beta-Glucan
-
50C, pH 5.5, endoglucanase EG44
84
-
beta-Glucan
-
50C, pH 5.5, endoglucanase EG51
0.0013
-
Carboxymethyl cellulose
-
pH 7.0, 37C, mutant D392A
-
0.0051
-
Carboxymethyl cellulose
-
pH 7.0, 37C, mutant D392S
-
0.0059
-
Carboxymethyl cellulose
-
pH 7.0, 37C, mutant D392N
-
0.0073
-
Carboxymethyl cellulose
-
pH 7.0, 37C, mutant D252C
-
0.014
-
Carboxymethyl cellulose
-
pH 7.0, 37C, oxidized mutant D252C
-
0.0146
-
Carboxymethyl cellulose
-
pH 7.0, 37C, mutant D216C
-
0.029
-
Carboxymethyl cellulose
-
pH 7.0, 37C, oxidized mutant D216C
-
0.227
-
Carboxymethyl cellulose
-
pH 7.0, 37C, mutant D216N
-
0.994
-
Carboxymethyl cellulose
-
pH 7.0, 37C, mutant D392C
-
3.57
-
Carboxymethyl cellulose
-
pH 7.0, 37C, oxidized mutant D392C
-
5.81
-
Carboxymethyl cellulose
-
pH 7.0, 37C, H2O2-treated wild-type CenA
-
5.92
-
Carboxymethyl cellulose
-
pH 7.0, 37C, wild-type CenA
-
7.4
-
Carboxymethyl cellulose
-
in presence of 25 mM
-
15
-
Carboxymethyl cellulose
-
50C, pH 5.5, endoglucanase EG25
-
19
-
Carboxymethyl cellulose
-
50C, pH 5.5, endoglucanase EG28
-
22
-
Carboxymethyl cellulose
-
50C, pH 5.5, endoglucanase EG60
-
27
-
Carboxymethyl cellulose
-
50C, pH 5.5, endoglucanase EG47
-
54
-
Carboxymethyl cellulose
-
50C, pH 5.5, endoglucanase EG44
-
54
-
Carboxymethyl cellulose
-
50C, pH 5.5, endoglucanase EG51
-
84.4
-
Carboxymethyl cellulose
-
in presence of 250 mM
-
163
-
Carboxymethyl cellulose
G3BJX5
Rucel5B, pH 6.5, 60C
-
190.1
-
Carboxymethyl cellulose
-
in presence of 25 mM
-
217
-
Carboxymethyl cellulose
-
enzyme from strain NIAB442
-
278
-
Carboxymethyl cellulose
-
enzyme from deoxyglucose- and streptomycin-resistant mutant 51 Smr
-
26.4
-
Carboxymethylcellulose
-
pH 8.5, 40C
-
246
-
Carboxymethylcellulose
-
wild-type
-
318
-
Carboxymethylcellulose
-
in presence of 1.5 mM Mn2+
-
0.39
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 3, 80C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA; pH and temperature not specified in the publication, hybrid protein (CelA-SSO1949-CelA, consisting of 262 amino acids) based on beta-endoglucanase from Sulfolobus solfataricus P2 and beta-endoglucanase from Thermotoga maritima
-
0.39
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 3, 80C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
-
19
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 3, 80C, native enzyme
-
0.003
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme I162A
0.006
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme H155A
0.01
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme E201Q; pH 5-6, 50C, mutant enzyme H297N; pH 5-6, 50C, mutant enzyme Y299F
0.036
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme E163A
0.037
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme P164A
0.083
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme R156A
0.092
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme G158A
0.11
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme D385N
0.115
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme I157A
0.129
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme T160A
0.144
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme C159A
0.157
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, wild-type enzyme
0.167
-
p-nitrophenyl cellobiose
-
pH 5.5, 50C, mutant enzyme H161A
0.24
-
p-nitrophenyl cellobiose
-
50C, mutant enzyme C106A/C159A
0.41
-
p-nitrophenyl cellobiose
-
50C, wild-type enzyme
0.41
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, wild-type enzyme
0.43
-
p-nitrophenyl cellobiose
-
50C, mutant enzyme C372A/C412A
0.47
-
p-nitrophenyl cellobiose
-
50C, mutant enzyme C106A/C159A/C372A/C412A
0.75
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme lacking 5 residues at the C-terminus
0.78
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme DELTAQ1-G5
0.91
-
p-nitrophenyl cellobiose
-
pH 5-6, 50C, mutant enzyme lacking 5 residues at the C-terminus and 5 residies at the N-terminus
0.01
-
p-nitrophenyl-beta-D-cellobioside
-
-
0.18
-
p-nitrophenyl-beta-D-cellobioside
-
-
437
-
Carboxymethylcellulose
-
the turnover rate (kcat) of Egl330 increased by 78% compared to wild-type
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
356 1/s, one unit defined as the release of 1 mircomol of glucose equivalent per minute from carboxymethylcellulose
-
additional information
-
additional information
-
kinetic analysis shows both the turnover rate (kcat) and the catalytic efficiency (kcat/Km) of Egl330 increased for the substrate carboxymethylcellulose compared to Egl499
-
additional information
-
additional information
-
kcat-values of N-terminal and C-terminal deletion mutants
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.06
-
4-nitrophenyl beta-D-glucoside
-
pH 6.0, 95C
4738
1.51
-
4-nitrophenyl cellobioside
-
pH 6.0, 70C
214887
8.7
-
4-nitrophenyl cellobioside
-
pH 6.0, 95C
214887
6.5
-
4-nitrophenyl cellopentaoside
-
pH 6.0, 95C
329261
49
-
4-nitrophenyl cellotetraoside
-
pH 6.0, 95C
329260
229
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 3, 80C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
0
230
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH and temperature not specified in the publication, hybrid protein (CelA-SSO1949-CelA, consisting of 262 amino acids) based on beta-endoglucanase from Sulfolobus solfataricus P2 and beta-endoglucanase from Thermotoga maritima
0
3455
-
N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate
-
pH 3, 80C, native enzyme
0
220
-
4-nitrophenyl cellotrioside
-
pH 6.0, 95C
316727
additional information
-
additional information
-
kcat/KM-values of N-terminal and C-terminal deletion mutants
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.6
-
cellobiose
-
substrate: bacterial celulose, endoglucanase Cel 7B
3
-
cellobiose
-
pH 6.0, 95C
9.08
-
cellobiose
-
pH 5.5, 50C
11
-
cellobiose
-
substrate: amorphous celulose, endoglucanase Cel 7B
34
-
cellobiose
-
substrate: amorphous celulose, endoglucanase Cel 34
0.1
-
D-glucono-1,5-lactone
-
pH 6.0, 95C
90
-
D-glucose
-
pH 6.0, 95C
1.6
-
laminaribiose
-
pH 6.0, 95C
0.574
-
sodium dodecyl benzene sulfonate
-
-
0.051
-
sodium hypochlorite
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.039
-
sodium hypochlorite
B5BNY1, -
-
additional information
-
additional information
B5BNY1, -
0.303 mM for linear alkyl benzene sulfonate
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.018
-
-
TfCel9A wild-type, activity on 600 microM G5
0.023
-
-
homogenate
0.037
-
-
TfCel9A W256A mutant, activity on 600 microM G5
0.166
-
-
after DEAE anion exchange chromatography
0.23
-
-
homogenate
0.258
-
-
-
0.29
-
-
cells in flasks
0.367
-
-
midgut
0.56
-
-
cells in bioreactor
0.669
-
-
hindgut
0.673
-
-
foregut proventriculus
0.71
-
-
foregut crop
0.8
-
-
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 3, 80C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA; substrate: N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH and temperature not specified in the publication, hybrid protein (CelA-SSO1949-CelA, consisting of 262 amino acids) based on beta-endoglucanase from Sulfolobus solfataricus P2 and beta-endoglucanase from Thermotoga maritima
0.8
-
-
substrate N-[2-N-[(S-(4-deoxy-4-dimethylamino-phenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 3, 80C, chimeric fusion protein CelA-SSO1949-CelA, that consists of 70 amino acids CelA followed by 163 amino acids SSO1949 and 29 amino acids CelA
1.05
-
A4UU22
CM cellulose
1.2
-
-
substrate: N-[2-N-[(S-(4-deoxy-4-dimethylaminophenylazophenylthioureido-beta-D-glucopyranosyl)-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl)-2-thioacetyl]aminoethyl]-1-naphthylamine-5-sulfonate, pH 1.8, 80C
1.254
-
-
salivary gland
1.91
-
-
Cel5A, carboxymethyl cellulose as substrate
2.8
-
Q97YG7
debranched arabinan, relative activity 51%
2.9
-
Q97YG7
arabinan, relative activity 53%
3.88
-
-
M44-11 mutant, carboxymethyl cellulose as substrate
4
-
Q97YG7
birchwood xylan, relative activity 73%
4.2
-
Q97YG7
oat spelt xylan, relative activity 76%
4.6
-
Q97YG7
beechwood xylan, relative activity 84%
4.84
-
-
S75 mutant, carboxymethyl cellulose as substrate
4.88
-
-
S78 mutant, carboxymethyl cellulose as substrate
4.93
-
A4UU22
carboxymethyl cellulose sodium salt
5.13
-
-
after DEAE anion exchange chromatography
5.5
-
Q97YG7
carboxymethylcellulose, relative activity 100%
7.43
-
-
after gel filtration chromatography
11.02
-
-
carboxymethyl cellulase, pH 4.8, 45C, culture from cutter-milled rice straw as carbon source
11.61
-
-
after DEAE anion exchange chromatography
12.3
-
-
carboxymethyl cellulase supernatant, glucose as a substrate
12.74
-
-
after gel filtration chromatography; last purification step
13.06
-
-
-
16.5
-
-
catalytic domain of endoglucanase G
16.76
-
-
carboxymethyl cellulase, pH 4.8, 45C, culture from Solka Floc as carbon source
18.63
-
-
carboxymethyl cellulase, pH 4.8, 45C, culture from dry ball-milled rice straw as carbon source
19.09
-
-
after gel filtration chromatography; last purification step
19.73
-