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Literature summary for 3.2.1.4 extracted from
- Production, purification and properties of endoglucanase from a newly isolated strain of Mucor circinelloides (2004), Proc. Biochem., 39, 1871-1876.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-cyclodextrin | 10 mM, activity is enhanced by 23% | Mucor circinelloides |  |
| gamma-cyclodextrin | 10 mM, activity is enhanced by 137% | Mucor circinelloides | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CoCl2 | 0.5 mM, activity is enhanced by 44% | Mucor circinelloides | |
| MgCl2 | 5 mM, activity is enhanced by 27% | Mucor circinelloides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Mucor circinelloides |
| 27000 | - |
1 * 27000, SDS-PAGE | Mucor circinelloides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mucor circinelloides | - |
NRRL 26519 | - |
| Mucor circinelloides NRRL 26519 | - |
NRRL 26519 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mucor circinelloides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Mucor circinelloides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 43 | - |
- |
Mucor circinelloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carboxymethyl cellulose + H2O | - |
Mucor circinelloides | ? | no glucose, cellobiose and short chain celloolifgosaccharides are formed | ? | |
| carboxymethyl cellulose + H2O | - |
Mucor circinelloides NRRL 26519 | ? | no glucose, cellobiose and short chain celloolifgosaccharides are formed | ? | |
| insoluble cellulose + H2O | e.g. Avicel, Solka-floc, Sigmacell 50 | Mucor circinelloides | ? | no glucose, cellobiose and short chain celloolifgosaccharides are formed | ? | |
| insoluble cellulose + H2O | e.g. Avicel, Solka-floc, Sigmacell 50 | Mucor circinelloides NRRL 26519 | ? | no glucose, cellobiose and short chain celloolifgosaccharides are formed | ? | |
| additional information | no degradation of oat spelt xylan and larch wood xylan | Mucor circinelloides | ? | - |
? | |
| additional information | no degradation of oat spelt xylan and larch wood xylan | Mucor circinelloides NRRL 26519 | ? | - |
? | |
| soluble cellodextrin + H2O | - |
Mucor circinelloides | ? | no glucose, cellobiose and short chain cellooligosaccharides are formed | ? | |
| soluble cellodextrin + H2O | - |
Mucor circinelloides NRRL 26519 | ? | no glucose, cellobiose and short chain cellooligosaccharides are formed | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 27000, SDS-PAGE | Mucor circinelloides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Mucor circinelloides |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | 30°C: about 60% of maximal activity, 70°C: about 30% of maximal activity | Mucor circinelloides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, stable up to | Mucor circinelloides |
| 70 | - |
30 min, 10% loss of activity | Mucor circinelloides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Mucor circinelloides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 7.5 | pH 2.5: about 50% of maximal activity, pH 7.5: about 80% of maximal activity | Mucor circinelloides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | stable in the range | Mucor circinelloides |
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